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Short-Term High Fat Feeding Increases Organ Injury and Mortality After Polymicrobial Sepsis

The purpose of this study was to examine the effect of short-term high fat feeding on the inflammatory response in polymicrobial sepsis. Male C57BL/6 mice at six-weeks of age were randomized to a high-fat diet (HFD) (60% kcal fat) or control diet (CD) (16% kcal fat) for 3 weeks. After 3 weeks of fee...

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Autores principales: Kaplan, Jennifer M., Nowell, Marchele, Lahni, Patrick, O’Connor, Michael, Hake, Paul W., Zingarelli, Basilia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3366015/
https://www.ncbi.nlm.nih.gov/pubmed/22334256
http://dx.doi.org/10.1038/oby.2012.40
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author Kaplan, Jennifer M.
Nowell, Marchele
Lahni, Patrick
O’Connor, Michael
Hake, Paul W.
Zingarelli, Basilia
author_facet Kaplan, Jennifer M.
Nowell, Marchele
Lahni, Patrick
O’Connor, Michael
Hake, Paul W.
Zingarelli, Basilia
author_sort Kaplan, Jennifer M.
collection PubMed
description The purpose of this study was to examine the effect of short-term high fat feeding on the inflammatory response in polymicrobial sepsis. Male C57BL/6 mice at six-weeks of age were randomized to a high-fat diet (HFD) (60% kcal fat) or control diet (CD) (16% kcal fat) for 3 weeks. After 3 weeks of feeding, sepsis was induced by cecal ligation and puncture (CLP) and animals were monitored for survival. In a separate experiment, after 3 weeks of feeding mice underwent CLP and were sacrificed at various time-points thereafter. Tissue was collected for biochemical studies. Mice fed a HFD gained more weight and had a greater fat mass compared to CD-fed mice. Mice on a HFD had a lower probability of survival and more severe lung injury compared with CD-fed mice following sepsis. Myeloperoxidase activity, an indicator of neutrophil infiltration, was increased in the lung and liver after CLP in HFD-fed mice compared with CD (p<0.05). The plasma cytokines tumor necrosis factor-α (TNFα) and interleukin (IL)-6 were increased in both groups after CLP, however TNFα and IL-6 levels were lower in HFD mice at 3h after CLP compared with CD and consistent with lung, but not liver, mRNA expression. Leptin levels were higher in HFD-fed mice at 18h after sepsis compared to baseline levels (p<0.05). Polymicrobial sepsis increased hepatic nuclear factor-κB (NF-κB) activation in HFD-fed mice after CLP vs. CD-fed mice. Short duration high fat feeding increases mortality and organ injury following polymicrobial sepsis. These effects correspond to changes in NF-κB.
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spelling pubmed-33660152013-04-01 Short-Term High Fat Feeding Increases Organ Injury and Mortality After Polymicrobial Sepsis Kaplan, Jennifer M. Nowell, Marchele Lahni, Patrick O’Connor, Michael Hake, Paul W. Zingarelli, Basilia Obesity (Silver Spring) Article The purpose of this study was to examine the effect of short-term high fat feeding on the inflammatory response in polymicrobial sepsis. Male C57BL/6 mice at six-weeks of age were randomized to a high-fat diet (HFD) (60% kcal fat) or control diet (CD) (16% kcal fat) for 3 weeks. After 3 weeks of feeding, sepsis was induced by cecal ligation and puncture (CLP) and animals were monitored for survival. In a separate experiment, after 3 weeks of feeding mice underwent CLP and were sacrificed at various time-points thereafter. Tissue was collected for biochemical studies. Mice fed a HFD gained more weight and had a greater fat mass compared to CD-fed mice. Mice on a HFD had a lower probability of survival and more severe lung injury compared with CD-fed mice following sepsis. Myeloperoxidase activity, an indicator of neutrophil infiltration, was increased in the lung and liver after CLP in HFD-fed mice compared with CD (p<0.05). The plasma cytokines tumor necrosis factor-α (TNFα) and interleukin (IL)-6 were increased in both groups after CLP, however TNFα and IL-6 levels were lower in HFD mice at 3h after CLP compared with CD and consistent with lung, but not liver, mRNA expression. Leptin levels were higher in HFD-fed mice at 18h after sepsis compared to baseline levels (p<0.05). Polymicrobial sepsis increased hepatic nuclear factor-κB (NF-κB) activation in HFD-fed mice after CLP vs. CD-fed mice. Short duration high fat feeding increases mortality and organ injury following polymicrobial sepsis. These effects correspond to changes in NF-κB. 2012-02-15 2012-10 /pmc/articles/PMC3366015/ /pubmed/22334256 http://dx.doi.org/10.1038/oby.2012.40 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Kaplan, Jennifer M.
Nowell, Marchele
Lahni, Patrick
O’Connor, Michael
Hake, Paul W.
Zingarelli, Basilia
Short-Term High Fat Feeding Increases Organ Injury and Mortality After Polymicrobial Sepsis
title Short-Term High Fat Feeding Increases Organ Injury and Mortality After Polymicrobial Sepsis
title_full Short-Term High Fat Feeding Increases Organ Injury and Mortality After Polymicrobial Sepsis
title_fullStr Short-Term High Fat Feeding Increases Organ Injury and Mortality After Polymicrobial Sepsis
title_full_unstemmed Short-Term High Fat Feeding Increases Organ Injury and Mortality After Polymicrobial Sepsis
title_short Short-Term High Fat Feeding Increases Organ Injury and Mortality After Polymicrobial Sepsis
title_sort short-term high fat feeding increases organ injury and mortality after polymicrobial sepsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3366015/
https://www.ncbi.nlm.nih.gov/pubmed/22334256
http://dx.doi.org/10.1038/oby.2012.40
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