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Properties and Biocompatibility of Chitosan and Silk Fibroin Blend Films for Application in Skin Tissue Engineering

Chitosan/silk fibroin (CS/SF) blend films were prepared and evaluated for feasibility of using the films as biomaterial for skin tissue engineering application. Fourier transform infrared spectroscopy and differential scanning calorimetry analysis indicated chemical interaction between chitosan and...

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Autores principales: Luangbudnark, Witoo, Viyoch, Jarupa, Laupattarakasem, Wiroon, Surakunprapha, Palakorn, Laupattarakasem, Pisamai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific World Journal 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3366240/
https://www.ncbi.nlm.nih.gov/pubmed/22701367
http://dx.doi.org/10.1100/2012/697201
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author Luangbudnark, Witoo
Viyoch, Jarupa
Laupattarakasem, Wiroon
Surakunprapha, Palakorn
Laupattarakasem, Pisamai
author_facet Luangbudnark, Witoo
Viyoch, Jarupa
Laupattarakasem, Wiroon
Surakunprapha, Palakorn
Laupattarakasem, Pisamai
author_sort Luangbudnark, Witoo
collection PubMed
description Chitosan/silk fibroin (CS/SF) blend films were prepared and evaluated for feasibility of using the films as biomaterial for skin tissue engineering application. Fourier transform infrared spectroscopy and differential scanning calorimetry analysis indicated chemical interaction between chitosan and fibroin. Chitosan enhanced β-sheet conformation of fibroin and resulted in shifting of thermal degradation of the films. Flexibility, swelling index, and enzyme degradation were also increased by the chitosan content of the blend films. Biocompatibility of the blend films was determined by cultivation with fibroblast cells. All films showed no cytotoxicity by XTT assay. Fibroblast cells spread on CS/SF films via dendritic extensions, and cell-cell interactions were noted. Cell proliferation on CS/SF films was also demonstrated, and their phenotype was examined by the expression of collagen type I gene. These results showed possibility of using the CS/SF films as a supporting material for further study on skin tissue engineering.
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spelling pubmed-33662402012-06-14 Properties and Biocompatibility of Chitosan and Silk Fibroin Blend Films for Application in Skin Tissue Engineering Luangbudnark, Witoo Viyoch, Jarupa Laupattarakasem, Wiroon Surakunprapha, Palakorn Laupattarakasem, Pisamai ScientificWorldJournal Research Article Chitosan/silk fibroin (CS/SF) blend films were prepared and evaluated for feasibility of using the films as biomaterial for skin tissue engineering application. Fourier transform infrared spectroscopy and differential scanning calorimetry analysis indicated chemical interaction between chitosan and fibroin. Chitosan enhanced β-sheet conformation of fibroin and resulted in shifting of thermal degradation of the films. Flexibility, swelling index, and enzyme degradation were also increased by the chitosan content of the blend films. Biocompatibility of the blend films was determined by cultivation with fibroblast cells. All films showed no cytotoxicity by XTT assay. Fibroblast cells spread on CS/SF films via dendritic extensions, and cell-cell interactions were noted. Cell proliferation on CS/SF films was also demonstrated, and their phenotype was examined by the expression of collagen type I gene. These results showed possibility of using the CS/SF films as a supporting material for further study on skin tissue engineering. The Scientific World Journal 2012-05-22 /pmc/articles/PMC3366240/ /pubmed/22701367 http://dx.doi.org/10.1100/2012/697201 Text en Copyright © 2012 Witoo Luangbudnark et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Luangbudnark, Witoo
Viyoch, Jarupa
Laupattarakasem, Wiroon
Surakunprapha, Palakorn
Laupattarakasem, Pisamai
Properties and Biocompatibility of Chitosan and Silk Fibroin Blend Films for Application in Skin Tissue Engineering
title Properties and Biocompatibility of Chitosan and Silk Fibroin Blend Films for Application in Skin Tissue Engineering
title_full Properties and Biocompatibility of Chitosan and Silk Fibroin Blend Films for Application in Skin Tissue Engineering
title_fullStr Properties and Biocompatibility of Chitosan and Silk Fibroin Blend Films for Application in Skin Tissue Engineering
title_full_unstemmed Properties and Biocompatibility of Chitosan and Silk Fibroin Blend Films for Application in Skin Tissue Engineering
title_short Properties and Biocompatibility of Chitosan and Silk Fibroin Blend Films for Application in Skin Tissue Engineering
title_sort properties and biocompatibility of chitosan and silk fibroin blend films for application in skin tissue engineering
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3366240/
https://www.ncbi.nlm.nih.gov/pubmed/22701367
http://dx.doi.org/10.1100/2012/697201
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