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Fine Mapping of the Bsr1 Barley Stripe Mosaic Virus Resistance Gene in the Model Grass Brachypodium distachyon

The ND18 strain of Barley stripe mosaic virus (BSMV) infects several lines of Brachypodium distachyon, a recently developed model system for genomics research in cereals. Among the inbred lines tested, Bd3-1 is highly resistant at 20 to 25°C, whereas Bd21 is susceptible and infection results in an i...

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Autores principales: Cui, Yu, Lee, Mi Yeon, Huo, Naxin, Bragg, Jennifer, Yan, Lijie, Yuan, Cheng, Li, Cui, Holditch, Sara J., Xie, Jingzhong, Luo, Ming-Cheng, Li, Dawei, Yu, Jialin, Martin, Joel, Schackwitz, Wendy, Gu, Yong Qiang, Vogel, John P., Jackson, Andrew O., Liu, Zhiyong, Garvin, David F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3366947/
https://www.ncbi.nlm.nih.gov/pubmed/22675544
http://dx.doi.org/10.1371/journal.pone.0038333
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author Cui, Yu
Lee, Mi Yeon
Huo, Naxin
Bragg, Jennifer
Yan, Lijie
Yuan, Cheng
Li, Cui
Holditch, Sara J.
Xie, Jingzhong
Luo, Ming-Cheng
Li, Dawei
Yu, Jialin
Martin, Joel
Schackwitz, Wendy
Gu, Yong Qiang
Vogel, John P.
Jackson, Andrew O.
Liu, Zhiyong
Garvin, David F.
author_facet Cui, Yu
Lee, Mi Yeon
Huo, Naxin
Bragg, Jennifer
Yan, Lijie
Yuan, Cheng
Li, Cui
Holditch, Sara J.
Xie, Jingzhong
Luo, Ming-Cheng
Li, Dawei
Yu, Jialin
Martin, Joel
Schackwitz, Wendy
Gu, Yong Qiang
Vogel, John P.
Jackson, Andrew O.
Liu, Zhiyong
Garvin, David F.
author_sort Cui, Yu
collection PubMed
description The ND18 strain of Barley stripe mosaic virus (BSMV) infects several lines of Brachypodium distachyon, a recently developed model system for genomics research in cereals. Among the inbred lines tested, Bd3-1 is highly resistant at 20 to 25°C, whereas Bd21 is susceptible and infection results in an intense mosaic phenotype accompanied by high levels of replicating virus. We generated an F(6∶7) recombinant inbred line (RIL) population from a cross between Bd3-1 and Bd21 and used the RILs, and an F(2) population of a second Bd21 × Bd3-1 cross to evaluate the inheritance of resistance. The results indicate that resistance segregates as expected for a single dominant gene, which we have designated Barley stripe mosaic virus resistance 1 (Bsr1). We constructed a genetic linkage map of the RIL population using SNP markers to map this gene to within 705 Kb of the distal end of the top of chromosome 3. Additional CAPS and Indel markers were used to fine map Bsr1 to a 23 Kb interval containing five putative genes. Our study demonstrates the power of using RILs to rapidly map the genetic determinants of BSMV resistance in Brachypodium. Moreover, the RILs and their associated genetic map, when combined with the complete genomic sequence of Brachypodium, provide new resources for genetic analyses of many other traits.
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spelling pubmed-33669472012-06-06 Fine Mapping of the Bsr1 Barley Stripe Mosaic Virus Resistance Gene in the Model Grass Brachypodium distachyon Cui, Yu Lee, Mi Yeon Huo, Naxin Bragg, Jennifer Yan, Lijie Yuan, Cheng Li, Cui Holditch, Sara J. Xie, Jingzhong Luo, Ming-Cheng Li, Dawei Yu, Jialin Martin, Joel Schackwitz, Wendy Gu, Yong Qiang Vogel, John P. Jackson, Andrew O. Liu, Zhiyong Garvin, David F. PLoS One Research Article The ND18 strain of Barley stripe mosaic virus (BSMV) infects several lines of Brachypodium distachyon, a recently developed model system for genomics research in cereals. Among the inbred lines tested, Bd3-1 is highly resistant at 20 to 25°C, whereas Bd21 is susceptible and infection results in an intense mosaic phenotype accompanied by high levels of replicating virus. We generated an F(6∶7) recombinant inbred line (RIL) population from a cross between Bd3-1 and Bd21 and used the RILs, and an F(2) population of a second Bd21 × Bd3-1 cross to evaluate the inheritance of resistance. The results indicate that resistance segregates as expected for a single dominant gene, which we have designated Barley stripe mosaic virus resistance 1 (Bsr1). We constructed a genetic linkage map of the RIL population using SNP markers to map this gene to within 705 Kb of the distal end of the top of chromosome 3. Additional CAPS and Indel markers were used to fine map Bsr1 to a 23 Kb interval containing five putative genes. Our study demonstrates the power of using RILs to rapidly map the genetic determinants of BSMV resistance in Brachypodium. Moreover, the RILs and their associated genetic map, when combined with the complete genomic sequence of Brachypodium, provide new resources for genetic analyses of many other traits. Public Library of Science 2012-06-04 /pmc/articles/PMC3366947/ /pubmed/22675544 http://dx.doi.org/10.1371/journal.pone.0038333 Text en Cui et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Cui, Yu
Lee, Mi Yeon
Huo, Naxin
Bragg, Jennifer
Yan, Lijie
Yuan, Cheng
Li, Cui
Holditch, Sara J.
Xie, Jingzhong
Luo, Ming-Cheng
Li, Dawei
Yu, Jialin
Martin, Joel
Schackwitz, Wendy
Gu, Yong Qiang
Vogel, John P.
Jackson, Andrew O.
Liu, Zhiyong
Garvin, David F.
Fine Mapping of the Bsr1 Barley Stripe Mosaic Virus Resistance Gene in the Model Grass Brachypodium distachyon
title Fine Mapping of the Bsr1 Barley Stripe Mosaic Virus Resistance Gene in the Model Grass Brachypodium distachyon
title_full Fine Mapping of the Bsr1 Barley Stripe Mosaic Virus Resistance Gene in the Model Grass Brachypodium distachyon
title_fullStr Fine Mapping of the Bsr1 Barley Stripe Mosaic Virus Resistance Gene in the Model Grass Brachypodium distachyon
title_full_unstemmed Fine Mapping of the Bsr1 Barley Stripe Mosaic Virus Resistance Gene in the Model Grass Brachypodium distachyon
title_short Fine Mapping of the Bsr1 Barley Stripe Mosaic Virus Resistance Gene in the Model Grass Brachypodium distachyon
title_sort fine mapping of the bsr1 barley stripe mosaic virus resistance gene in the model grass brachypodium distachyon
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3366947/
https://www.ncbi.nlm.nih.gov/pubmed/22675544
http://dx.doi.org/10.1371/journal.pone.0038333
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