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Tuning the Drug Efflux Activity of an ABC Transporter in vivo by in vitro Selected DARPin Binders

ABC transporters use the energy from binding and hydrolysis of ATP to import or extrude substrates across the membrane. Using ribosome display, we raised designed ankyrin repeat proteins (DARPins) against detergent solubilized LmrCD, a heterodimeric multidrug ABC exporter from Lactococcus lactis. Se...

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Detalles Bibliográficos
Autores principales:	Seeger, Markus A., Mittal, Anshumali, Velamakanni, Saroj, Hohl, Michael, Schauer, Stefan, Salaa, Ihsene, Grütter, Markus G., van Veen, Hendrik W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3366976/ https://www.ncbi.nlm.nih.gov/pubmed/22675494 http://dx.doi.org/10.1371/journal.pone.0037845

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