Cargando…

Novel RNA base pair with higher specificity using single selenium atom

Specificity of nucleobase pairing provides essential foundation for genetic information storage, replication, transcription and translation in all living organisms. However, the wobble base pairs, where U in RNA (or T in DNA) pairs with G instead of A, might compromise the high specificity of the ba...

Descripción completa

Detalles Bibliográficos
Autores principales: Sun, Huiyan, Sheng, Jia, Hassan, Abdalla E. A., Jiang, Sibo, Gan, Jianhua, Huang, Zhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3367167/
https://www.ncbi.nlm.nih.gov/pubmed/22323523
http://dx.doi.org/10.1093/nar/gks010
_version_ 1782234811022180352
author Sun, Huiyan
Sheng, Jia
Hassan, Abdalla E. A.
Jiang, Sibo
Gan, Jianhua
Huang, Zhen
author_facet Sun, Huiyan
Sheng, Jia
Hassan, Abdalla E. A.
Jiang, Sibo
Gan, Jianhua
Huang, Zhen
author_sort Sun, Huiyan
collection PubMed
description Specificity of nucleobase pairing provides essential foundation for genetic information storage, replication, transcription and translation in all living organisms. However, the wobble base pairs, where U in RNA (or T in DNA) pairs with G instead of A, might compromise the high specificity of the base pairing. The U/G wobble pairing is ubiquitous in RNA, especially in non-coding RNA. In order to increase U/A pairing specificity, we have hypothesized to discriminate against U/G wobble pair by tailoring the steric and electronic effects at the 2-exo position of uridine and replacing the 2-exo oxygen with a selenium atom. We report here the first synthesis of the 2-Se-U-RNAs as well as the 2-Se-uridine ((Se)U) phosphoramidite. Our biophysical and structural studies of the (Se)U-RNAs indicate that this single atom replacement can indeed create a novel U/A base pair with higher specificity than the natural one. We reveal that the (Se)U/A pair maintains a structure virtually identical to the native U/A base pair, while discriminating against U/G wobble pair. This oxygen replacement with selenium offers a unique chemical strategy to enhance the base pairing specificity at the atomic level.
format Online
Article
Text
id pubmed-3367167
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-33671672012-06-05 Novel RNA base pair with higher specificity using single selenium atom Sun, Huiyan Sheng, Jia Hassan, Abdalla E. A. Jiang, Sibo Gan, Jianhua Huang, Zhen Nucleic Acids Res Synthetic Biology and Chemistry Specificity of nucleobase pairing provides essential foundation for genetic information storage, replication, transcription and translation in all living organisms. However, the wobble base pairs, where U in RNA (or T in DNA) pairs with G instead of A, might compromise the high specificity of the base pairing. The U/G wobble pairing is ubiquitous in RNA, especially in non-coding RNA. In order to increase U/A pairing specificity, we have hypothesized to discriminate against U/G wobble pair by tailoring the steric and electronic effects at the 2-exo position of uridine and replacing the 2-exo oxygen with a selenium atom. We report here the first synthesis of the 2-Se-U-RNAs as well as the 2-Se-uridine ((Se)U) phosphoramidite. Our biophysical and structural studies of the (Se)U-RNAs indicate that this single atom replacement can indeed create a novel U/A base pair with higher specificity than the natural one. We reveal that the (Se)U/A pair maintains a structure virtually identical to the native U/A base pair, while discriminating against U/G wobble pair. This oxygen replacement with selenium offers a unique chemical strategy to enhance the base pairing specificity at the atomic level. Oxford University Press 2012-06 2012-02-09 /pmc/articles/PMC3367167/ /pubmed/22323523 http://dx.doi.org/10.1093/nar/gks010 Text en © The Author(s) 2012. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Synthetic Biology and Chemistry
Sun, Huiyan
Sheng, Jia
Hassan, Abdalla E. A.
Jiang, Sibo
Gan, Jianhua
Huang, Zhen
Novel RNA base pair with higher specificity using single selenium atom
title Novel RNA base pair with higher specificity using single selenium atom
title_full Novel RNA base pair with higher specificity using single selenium atom
title_fullStr Novel RNA base pair with higher specificity using single selenium atom
title_full_unstemmed Novel RNA base pair with higher specificity using single selenium atom
title_short Novel RNA base pair with higher specificity using single selenium atom
title_sort novel rna base pair with higher specificity using single selenium atom
topic Synthetic Biology and Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3367167/
https://www.ncbi.nlm.nih.gov/pubmed/22323523
http://dx.doi.org/10.1093/nar/gks010
work_keys_str_mv AT sunhuiyan novelrnabasepairwithhigherspecificityusingsingleseleniumatom
AT shengjia novelrnabasepairwithhigherspecificityusingsingleseleniumatom
AT hassanabdallaea novelrnabasepairwithhigherspecificityusingsingleseleniumatom
AT jiangsibo novelrnabasepairwithhigherspecificityusingsingleseleniumatom
AT ganjianhua novelrnabasepairwithhigherspecificityusingsingleseleniumatom
AT huangzhen novelrnabasepairwithhigherspecificityusingsingleseleniumatom