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Novel RNA base pair with higher specificity using single selenium atom
Specificity of nucleobase pairing provides essential foundation for genetic information storage, replication, transcription and translation in all living organisms. However, the wobble base pairs, where U in RNA (or T in DNA) pairs with G instead of A, might compromise the high specificity of the ba...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3367167/ https://www.ncbi.nlm.nih.gov/pubmed/22323523 http://dx.doi.org/10.1093/nar/gks010 |
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author | Sun, Huiyan Sheng, Jia Hassan, Abdalla E. A. Jiang, Sibo Gan, Jianhua Huang, Zhen |
author_facet | Sun, Huiyan Sheng, Jia Hassan, Abdalla E. A. Jiang, Sibo Gan, Jianhua Huang, Zhen |
author_sort | Sun, Huiyan |
collection | PubMed |
description | Specificity of nucleobase pairing provides essential foundation for genetic information storage, replication, transcription and translation in all living organisms. However, the wobble base pairs, where U in RNA (or T in DNA) pairs with G instead of A, might compromise the high specificity of the base pairing. The U/G wobble pairing is ubiquitous in RNA, especially in non-coding RNA. In order to increase U/A pairing specificity, we have hypothesized to discriminate against U/G wobble pair by tailoring the steric and electronic effects at the 2-exo position of uridine and replacing the 2-exo oxygen with a selenium atom. We report here the first synthesis of the 2-Se-U-RNAs as well as the 2-Se-uridine ((Se)U) phosphoramidite. Our biophysical and structural studies of the (Se)U-RNAs indicate that this single atom replacement can indeed create a novel U/A base pair with higher specificity than the natural one. We reveal that the (Se)U/A pair maintains a structure virtually identical to the native U/A base pair, while discriminating against U/G wobble pair. This oxygen replacement with selenium offers a unique chemical strategy to enhance the base pairing specificity at the atomic level. |
format | Online Article Text |
id | pubmed-3367167 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-33671672012-06-05 Novel RNA base pair with higher specificity using single selenium atom Sun, Huiyan Sheng, Jia Hassan, Abdalla E. A. Jiang, Sibo Gan, Jianhua Huang, Zhen Nucleic Acids Res Synthetic Biology and Chemistry Specificity of nucleobase pairing provides essential foundation for genetic information storage, replication, transcription and translation in all living organisms. However, the wobble base pairs, where U in RNA (or T in DNA) pairs with G instead of A, might compromise the high specificity of the base pairing. The U/G wobble pairing is ubiquitous in RNA, especially in non-coding RNA. In order to increase U/A pairing specificity, we have hypothesized to discriminate against U/G wobble pair by tailoring the steric and electronic effects at the 2-exo position of uridine and replacing the 2-exo oxygen with a selenium atom. We report here the first synthesis of the 2-Se-U-RNAs as well as the 2-Se-uridine ((Se)U) phosphoramidite. Our biophysical and structural studies of the (Se)U-RNAs indicate that this single atom replacement can indeed create a novel U/A base pair with higher specificity than the natural one. We reveal that the (Se)U/A pair maintains a structure virtually identical to the native U/A base pair, while discriminating against U/G wobble pair. This oxygen replacement with selenium offers a unique chemical strategy to enhance the base pairing specificity at the atomic level. Oxford University Press 2012-06 2012-02-09 /pmc/articles/PMC3367167/ /pubmed/22323523 http://dx.doi.org/10.1093/nar/gks010 Text en © The Author(s) 2012. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Synthetic Biology and Chemistry Sun, Huiyan Sheng, Jia Hassan, Abdalla E. A. Jiang, Sibo Gan, Jianhua Huang, Zhen Novel RNA base pair with higher specificity using single selenium atom |
title | Novel RNA base pair with higher specificity using single selenium atom |
title_full | Novel RNA base pair with higher specificity using single selenium atom |
title_fullStr | Novel RNA base pair with higher specificity using single selenium atom |
title_full_unstemmed | Novel RNA base pair with higher specificity using single selenium atom |
title_short | Novel RNA base pair with higher specificity using single selenium atom |
title_sort | novel rna base pair with higher specificity using single selenium atom |
topic | Synthetic Biology and Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3367167/ https://www.ncbi.nlm.nih.gov/pubmed/22323523 http://dx.doi.org/10.1093/nar/gks010 |
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