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The global repressor FliZ antagonizes gene expression by σ(S)-containing RNA polymerase due to overlapping DNA binding specificity

FliZ, a global regulatory protein under the control of the flagellar master regulator FlhDC, was shown to antagonize σ(S)-dependent gene expression in Escherichia coli. Thereby it plays a pivotal role in the decision between alternative life-styles, i.e. FlhDC-controlled flagellum-based motility or...

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Autores principales: Pesavento, Christina, Hengge, Regine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3367168/
https://www.ncbi.nlm.nih.gov/pubmed/22323519
http://dx.doi.org/10.1093/nar/gks055
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author Pesavento, Christina
Hengge, Regine
author_facet Pesavento, Christina
Hengge, Regine
author_sort Pesavento, Christina
collection PubMed
description FliZ, a global regulatory protein under the control of the flagellar master regulator FlhDC, was shown to antagonize σ(S)-dependent gene expression in Escherichia coli. Thereby it plays a pivotal role in the decision between alternative life-styles, i.e. FlhDC-controlled flagellum-based motility or σ(S)-dependent curli fimbriae-mediated adhesion and biofilm formation. Here, we show that FliZ is an abundant DNA-binding protein that inhibits gene expression mediated by σ(S) by recognizing operator sequences that resemble the −10 region of σ(S)-dependent promoters. FliZ does so with a structural element that is similar to region 3.0 of σ(S). Within this element, R108 in FliZ corresponds to K173 in σ(S), which contacts a conserved cytosine at the −13 promoter position that is specific for σ(S)-dependent promoters. R108 as well as C(−13) are also crucial for DNA binding by FliZ. However, while a number of FliZ binding sites correspond to known σ(S)-dependent promoters, promoter activity is not a prerequisite for FliZ binding and repressor function. Thus, we demonstrate that FliZ also feedback-controls flagellar gene expression by binding to a site in the flhDC control region that shows similarity only to a −10 element of a σ(S)-dependent promoter, but does not function as a promoter.
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spelling pubmed-33671682012-06-05 The global repressor FliZ antagonizes gene expression by σ(S)-containing RNA polymerase due to overlapping DNA binding specificity Pesavento, Christina Hengge, Regine Nucleic Acids Res Gene Regulation, Chromatin and Epigenetics FliZ, a global regulatory protein under the control of the flagellar master regulator FlhDC, was shown to antagonize σ(S)-dependent gene expression in Escherichia coli. Thereby it plays a pivotal role in the decision between alternative life-styles, i.e. FlhDC-controlled flagellum-based motility or σ(S)-dependent curli fimbriae-mediated adhesion and biofilm formation. Here, we show that FliZ is an abundant DNA-binding protein that inhibits gene expression mediated by σ(S) by recognizing operator sequences that resemble the −10 region of σ(S)-dependent promoters. FliZ does so with a structural element that is similar to region 3.0 of σ(S). Within this element, R108 in FliZ corresponds to K173 in σ(S), which contacts a conserved cytosine at the −13 promoter position that is specific for σ(S)-dependent promoters. R108 as well as C(−13) are also crucial for DNA binding by FliZ. However, while a number of FliZ binding sites correspond to known σ(S)-dependent promoters, promoter activity is not a prerequisite for FliZ binding and repressor function. Thus, we demonstrate that FliZ also feedback-controls flagellar gene expression by binding to a site in the flhDC control region that shows similarity only to a −10 element of a σ(S)-dependent promoter, but does not function as a promoter. Oxford University Press 2012-06 2012-02-09 /pmc/articles/PMC3367168/ /pubmed/22323519 http://dx.doi.org/10.1093/nar/gks055 Text en © The Author(s) 2012. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Gene Regulation, Chromatin and Epigenetics
Pesavento, Christina
Hengge, Regine
The global repressor FliZ antagonizes gene expression by σ(S)-containing RNA polymerase due to overlapping DNA binding specificity
title The global repressor FliZ antagonizes gene expression by σ(S)-containing RNA polymerase due to overlapping DNA binding specificity
title_full The global repressor FliZ antagonizes gene expression by σ(S)-containing RNA polymerase due to overlapping DNA binding specificity
title_fullStr The global repressor FliZ antagonizes gene expression by σ(S)-containing RNA polymerase due to overlapping DNA binding specificity
title_full_unstemmed The global repressor FliZ antagonizes gene expression by σ(S)-containing RNA polymerase due to overlapping DNA binding specificity
title_short The global repressor FliZ antagonizes gene expression by σ(S)-containing RNA polymerase due to overlapping DNA binding specificity
title_sort global repressor fliz antagonizes gene expression by σ(s)-containing rna polymerase due to overlapping dna binding specificity
topic Gene Regulation, Chromatin and Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3367168/
https://www.ncbi.nlm.nih.gov/pubmed/22323519
http://dx.doi.org/10.1093/nar/gks055
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