Cargando…
Anticontractile Effect of Perivascular Adipose Tissue and Leptin are Reduced in Hypertension
Leptin causes vasodilatation both by endothelium-dependent and -independent mechanisms. Leptin is synthesized by perivascular adipose tissue (PVAT). The hypothesis of this study is that a decrease of leptin production in PVAT of spontaneously hypertensive rats (SHR) might contribute to a diminished...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Research Foundation
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3367267/ https://www.ncbi.nlm.nih.gov/pubmed/22679436 http://dx.doi.org/10.3389/fphar.2012.00103 |
_version_ | 1782234824715534336 |
---|---|
author | Gálvez-Prieto, Beatriz Somoza, Beatriz Gil-Ortega, Marta García-Prieto, Concha F. de las Heras, Ana I. González, M. Carmen Arribas, Silvia Aranguez, Isabel Bolbrinker, Juliane Kreutz, Reinhold Ruiz-Gayo, Mariano Fernández-Alfonso, Maria S. |
author_facet | Gálvez-Prieto, Beatriz Somoza, Beatriz Gil-Ortega, Marta García-Prieto, Concha F. de las Heras, Ana I. González, M. Carmen Arribas, Silvia Aranguez, Isabel Bolbrinker, Juliane Kreutz, Reinhold Ruiz-Gayo, Mariano Fernández-Alfonso, Maria S. |
author_sort | Gálvez-Prieto, Beatriz |
collection | PubMed |
description | Leptin causes vasodilatation both by endothelium-dependent and -independent mechanisms. Leptin is synthesized by perivascular adipose tissue (PVAT). The hypothesis of this study is that a decrease of leptin production in PVAT of spontaneously hypertensive rats (SHR) might contribute to a diminished paracrine anticontractile effect of the hormone. We have determined in aorta from Wistar-Kyoto (WKY) and SHR (i) leptin mRNA and protein levels in PVAT, (ii) the effect of leptin and PVAT on contractile responses, and (iii) leptin-induced relaxation and nitric oxide (NO) production. Leptin mRNA and protein expression were significantly lower in PVAT from SHR. Concentration-response curves to angiotensin II were significantly blunted in presence of PVAT as well as by exogenous leptin (10(−9) M) only in WKY. This anticontractile effect was endothelium-dependent. Vasodilatation induced by leptin was smaller in SHR than in WKY, and was also endothelium-dependent. Moreover, release of endothelial NO in response to acute leptin was higher in WKY compared to SHR, but completely abolished in the absence of endothelium. In conclusion, the reduced anticontractile effect of PVAT in SHR might be attributed to a reduced PVAT-derived leptin and to an abrogated effect of leptin on endothelial NO release probably due to an impaired activation of endothelial NO synthase. |
format | Online Article Text |
id | pubmed-3367267 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Frontiers Research Foundation |
record_format | MEDLINE/PubMed |
spelling | pubmed-33672672012-06-07 Anticontractile Effect of Perivascular Adipose Tissue and Leptin are Reduced in Hypertension Gálvez-Prieto, Beatriz Somoza, Beatriz Gil-Ortega, Marta García-Prieto, Concha F. de las Heras, Ana I. González, M. Carmen Arribas, Silvia Aranguez, Isabel Bolbrinker, Juliane Kreutz, Reinhold Ruiz-Gayo, Mariano Fernández-Alfonso, Maria S. Front Pharmacol Pharmacology Leptin causes vasodilatation both by endothelium-dependent and -independent mechanisms. Leptin is synthesized by perivascular adipose tissue (PVAT). The hypothesis of this study is that a decrease of leptin production in PVAT of spontaneously hypertensive rats (SHR) might contribute to a diminished paracrine anticontractile effect of the hormone. We have determined in aorta from Wistar-Kyoto (WKY) and SHR (i) leptin mRNA and protein levels in PVAT, (ii) the effect of leptin and PVAT on contractile responses, and (iii) leptin-induced relaxation and nitric oxide (NO) production. Leptin mRNA and protein expression were significantly lower in PVAT from SHR. Concentration-response curves to angiotensin II were significantly blunted in presence of PVAT as well as by exogenous leptin (10(−9) M) only in WKY. This anticontractile effect was endothelium-dependent. Vasodilatation induced by leptin was smaller in SHR than in WKY, and was also endothelium-dependent. Moreover, release of endothelial NO in response to acute leptin was higher in WKY compared to SHR, but completely abolished in the absence of endothelium. In conclusion, the reduced anticontractile effect of PVAT in SHR might be attributed to a reduced PVAT-derived leptin and to an abrogated effect of leptin on endothelial NO release probably due to an impaired activation of endothelial NO synthase. Frontiers Research Foundation 2012-06-05 /pmc/articles/PMC3367267/ /pubmed/22679436 http://dx.doi.org/10.3389/fphar.2012.00103 Text en Copyright © 2012 Gálvez-Prieto, Somoza, Gil-Ortega, García-Prieto, de las Heras, González, Arribas, Aranguez, Bolbrinker, Kreutz, Ruiz-Gayo and Fernández-Alfonso. http://www.frontiersin.org/licenseagreement This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited. |
spellingShingle | Pharmacology Gálvez-Prieto, Beatriz Somoza, Beatriz Gil-Ortega, Marta García-Prieto, Concha F. de las Heras, Ana I. González, M. Carmen Arribas, Silvia Aranguez, Isabel Bolbrinker, Juliane Kreutz, Reinhold Ruiz-Gayo, Mariano Fernández-Alfonso, Maria S. Anticontractile Effect of Perivascular Adipose Tissue and Leptin are Reduced in Hypertension |
title | Anticontractile Effect of Perivascular Adipose Tissue and Leptin are Reduced in Hypertension |
title_full | Anticontractile Effect of Perivascular Adipose Tissue and Leptin are Reduced in Hypertension |
title_fullStr | Anticontractile Effect of Perivascular Adipose Tissue and Leptin are Reduced in Hypertension |
title_full_unstemmed | Anticontractile Effect of Perivascular Adipose Tissue and Leptin are Reduced in Hypertension |
title_short | Anticontractile Effect of Perivascular Adipose Tissue and Leptin are Reduced in Hypertension |
title_sort | anticontractile effect of perivascular adipose tissue and leptin are reduced in hypertension |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3367267/ https://www.ncbi.nlm.nih.gov/pubmed/22679436 http://dx.doi.org/10.3389/fphar.2012.00103 |
work_keys_str_mv | AT galvezprietobeatriz anticontractileeffectofperivascularadiposetissueandleptinarereducedinhypertension AT somozabeatriz anticontractileeffectofperivascularadiposetissueandleptinarereducedinhypertension AT gilortegamarta anticontractileeffectofperivascularadiposetissueandleptinarereducedinhypertension AT garciaprietoconchaf anticontractileeffectofperivascularadiposetissueandleptinarereducedinhypertension AT delasherasanai anticontractileeffectofperivascularadiposetissueandleptinarereducedinhypertension AT gonzalezmcarmen anticontractileeffectofperivascularadiposetissueandleptinarereducedinhypertension AT arribassilvia anticontractileeffectofperivascularadiposetissueandleptinarereducedinhypertension AT aranguezisabel anticontractileeffectofperivascularadiposetissueandleptinarereducedinhypertension AT bolbrinkerjuliane anticontractileeffectofperivascularadiposetissueandleptinarereducedinhypertension AT kreutzreinhold anticontractileeffectofperivascularadiposetissueandleptinarereducedinhypertension AT ruizgayomariano anticontractileeffectofperivascularadiposetissueandleptinarereducedinhypertension AT fernandezalfonsomarias anticontractileeffectofperivascularadiposetissueandleptinarereducedinhypertension |