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Responses to Cortical Spreading Depression under Oxygen Deficiency

OBJECTIVES: Objectives: The effect of cortical spreading depression (CSD) on extracellular K(+) concentrations ([K(+)](e)), cerebral blood flow (CBF), mitochondrial NADH redox state and direct current (DC) potential was studied during normoxia and three pathological conditions: hypoxia, after NOS in...

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Autores principales: Sonn, J, Mayevsky, A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Open 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3367297/
https://www.ncbi.nlm.nih.gov/pubmed/22670162
http://dx.doi.org/10.2174/1874205X01206010006
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author Sonn, J
Mayevsky, A
author_facet Sonn, J
Mayevsky, A
author_sort Sonn, J
collection PubMed
description OBJECTIVES: Objectives: The effect of cortical spreading depression (CSD) on extracellular K(+) concentrations ([K(+)](e)), cerebral blood flow (CBF), mitochondrial NADH redox state and direct current (DC) potential was studied during normoxia and three pathological conditions: hypoxia, after NOS inhibition by L-NAME and partial ischemia. METHODS: A special device (MPA) was used for monitoring CSD wave propagation, containing: mitochondrial NADH redox state and reflected light, by a fluorometry technique; DC potential by Ag/AgCl electrodes; CBF by laser Doppler flowmetry; and [K(+)](e) by a mini-electrode. RESULTS AND DISCUSSION: 1. CSD under the 3 pathological conditions caused an initial increase in NADH and a further decrease in CBF during the first phase of CSD, indicating an imbalance between oxygen supply and demand as a result of the increase in oxygen requirements. . 2. The hyperperfusion phase in CBF was significantly reduced during hypoxia and ischemia showing a further decline in oxygen supply during CSD. . 3. CSD wave duration increased during the pathological conditions, showing a disturbance in energy production. 4. Extracellular K(+) levels during CSD, increased to identical levels during normoxia and during the three pathological groups, indicating correspondingly increase in oxygen demand. 5. The special design of the MPA enabled identifying differences in the simultaneous responses of the measured parameters, which may indicate changes in the interrelation between oxygen demand, oxygen supply and oxygen balance during CSD propagation, under the conditions tested. 6. In conclusion, brain oxygenation was found to be a critical factor in the responses of the brain to CSD.
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spelling pubmed-33672972012-06-05 Responses to Cortical Spreading Depression under Oxygen Deficiency Sonn, J Mayevsky, A Open Neurol J Article OBJECTIVES: Objectives: The effect of cortical spreading depression (CSD) on extracellular K(+) concentrations ([K(+)](e)), cerebral blood flow (CBF), mitochondrial NADH redox state and direct current (DC) potential was studied during normoxia and three pathological conditions: hypoxia, after NOS inhibition by L-NAME and partial ischemia. METHODS: A special device (MPA) was used for monitoring CSD wave propagation, containing: mitochondrial NADH redox state and reflected light, by a fluorometry technique; DC potential by Ag/AgCl electrodes; CBF by laser Doppler flowmetry; and [K(+)](e) by a mini-electrode. RESULTS AND DISCUSSION: 1. CSD under the 3 pathological conditions caused an initial increase in NADH and a further decrease in CBF during the first phase of CSD, indicating an imbalance between oxygen supply and demand as a result of the increase in oxygen requirements. . 2. The hyperperfusion phase in CBF was significantly reduced during hypoxia and ischemia showing a further decline in oxygen supply during CSD. . 3. CSD wave duration increased during the pathological conditions, showing a disturbance in energy production. 4. Extracellular K(+) levels during CSD, increased to identical levels during normoxia and during the three pathological groups, indicating correspondingly increase in oxygen demand. 5. The special design of the MPA enabled identifying differences in the simultaneous responses of the measured parameters, which may indicate changes in the interrelation between oxygen demand, oxygen supply and oxygen balance during CSD propagation, under the conditions tested. 6. In conclusion, brain oxygenation was found to be a critical factor in the responses of the brain to CSD. Bentham Open 2012-04-27 /pmc/articles/PMC3367297/ /pubmed/22670162 http://dx.doi.org/10.2174/1874205X01206010006 Text en © Sonn and Mayevsky; Licensee Bentham Open. http://creativecommons.org/licenses/by-nc/3.0/ This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Article
Sonn, J
Mayevsky, A
Responses to Cortical Spreading Depression under Oxygen Deficiency
title Responses to Cortical Spreading Depression under Oxygen Deficiency
title_full Responses to Cortical Spreading Depression under Oxygen Deficiency
title_fullStr Responses to Cortical Spreading Depression under Oxygen Deficiency
title_full_unstemmed Responses to Cortical Spreading Depression under Oxygen Deficiency
title_short Responses to Cortical Spreading Depression under Oxygen Deficiency
title_sort responses to cortical spreading depression under oxygen deficiency
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3367297/
https://www.ncbi.nlm.nih.gov/pubmed/22670162
http://dx.doi.org/10.2174/1874205X01206010006
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