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Portable microfluidic chip for detection of Escherichia coli in produce and blood

Pathogenic agents can lead to severe clinical outcomes such as food poisoning, infection of open wounds, particularly in burn injuries and sepsis. Rapid detection of these pathogens can monitor these infections in a timely manner improving clinical outcomes. Conventional bacterial detection methods,...

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Autores principales: Wang, ShuQi, Inci, Fatih, Chaunzwa, Tafadzwa L, Ramanujam, Ajay, Vasudevan, Aishwarya, Subramanian, Sathya, Chi Fai Ip, Alexander, Sridharan, Banupriya, Gurkan, Umut Atakan, Demirci, Utkan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3368510/
https://www.ncbi.nlm.nih.gov/pubmed/22679370
http://dx.doi.org/10.2147/IJN.S29629
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author Wang, ShuQi
Inci, Fatih
Chaunzwa, Tafadzwa L
Ramanujam, Ajay
Vasudevan, Aishwarya
Subramanian, Sathya
Chi Fai Ip, Alexander
Sridharan, Banupriya
Gurkan, Umut Atakan
Demirci, Utkan
author_facet Wang, ShuQi
Inci, Fatih
Chaunzwa, Tafadzwa L
Ramanujam, Ajay
Vasudevan, Aishwarya
Subramanian, Sathya
Chi Fai Ip, Alexander
Sridharan, Banupriya
Gurkan, Umut Atakan
Demirci, Utkan
author_sort Wang, ShuQi
collection PubMed
description Pathogenic agents can lead to severe clinical outcomes such as food poisoning, infection of open wounds, particularly in burn injuries and sepsis. Rapid detection of these pathogens can monitor these infections in a timely manner improving clinical outcomes. Conventional bacterial detection methods, such as agar plate culture or polymerase chain reaction, are time-consuming and dependent on complex and expensive instruments, which are not suitable for point-of-care (POC) settings. Therefore, there is an unmet need to develop a simple, rapid method for detection of pathogens such as Escherichia coli. Here, we present an immunobased microchip technology that can rapidly detect and quantify bacterial presence in various sources including physiologically relevant buffer solution (phosphate buffered saline [PBS]), blood, milk, and spinach. The microchip showed reliable capture of E. coli in PBS with an efficiency of 71.8% ± 5% at concentrations ranging from 50 to 4,000 CFUs/mL via lipopolysaccharide binding protein. The limits of detection of the microchip for PBS, blood, milk, and spinach samples were 50, 50, 50, and 500 CFUs/mL, respectively. The presented technology can be broadly applied to other pathogens at the POC, enabling various applications including surveillance of food supply and monitoring of bacteriology in patients with burn wounds.
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spelling pubmed-33685102012-06-07 Portable microfluidic chip for detection of Escherichia coli in produce and blood Wang, ShuQi Inci, Fatih Chaunzwa, Tafadzwa L Ramanujam, Ajay Vasudevan, Aishwarya Subramanian, Sathya Chi Fai Ip, Alexander Sridharan, Banupriya Gurkan, Umut Atakan Demirci, Utkan Int J Nanomedicine Original Research Pathogenic agents can lead to severe clinical outcomes such as food poisoning, infection of open wounds, particularly in burn injuries and sepsis. Rapid detection of these pathogens can monitor these infections in a timely manner improving clinical outcomes. Conventional bacterial detection methods, such as agar plate culture or polymerase chain reaction, are time-consuming and dependent on complex and expensive instruments, which are not suitable for point-of-care (POC) settings. Therefore, there is an unmet need to develop a simple, rapid method for detection of pathogens such as Escherichia coli. Here, we present an immunobased microchip technology that can rapidly detect and quantify bacterial presence in various sources including physiologically relevant buffer solution (phosphate buffered saline [PBS]), blood, milk, and spinach. The microchip showed reliable capture of E. coli in PBS with an efficiency of 71.8% ± 5% at concentrations ranging from 50 to 4,000 CFUs/mL via lipopolysaccharide binding protein. The limits of detection of the microchip for PBS, blood, milk, and spinach samples were 50, 50, 50, and 500 CFUs/mL, respectively. The presented technology can be broadly applied to other pathogens at the POC, enabling various applications including surveillance of food supply and monitoring of bacteriology in patients with burn wounds. Dove Medical Press 2012 2012-05-29 /pmc/articles/PMC3368510/ /pubmed/22679370 http://dx.doi.org/10.2147/IJN.S29629 Text en © 2012 Wang et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Wang, ShuQi
Inci, Fatih
Chaunzwa, Tafadzwa L
Ramanujam, Ajay
Vasudevan, Aishwarya
Subramanian, Sathya
Chi Fai Ip, Alexander
Sridharan, Banupriya
Gurkan, Umut Atakan
Demirci, Utkan
Portable microfluidic chip for detection of Escherichia coli in produce and blood
title Portable microfluidic chip for detection of Escherichia coli in produce and blood
title_full Portable microfluidic chip for detection of Escherichia coli in produce and blood
title_fullStr Portable microfluidic chip for detection of Escherichia coli in produce and blood
title_full_unstemmed Portable microfluidic chip for detection of Escherichia coli in produce and blood
title_short Portable microfluidic chip for detection of Escherichia coli in produce and blood
title_sort portable microfluidic chip for detection of escherichia coli in produce and blood
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3368510/
https://www.ncbi.nlm.nih.gov/pubmed/22679370
http://dx.doi.org/10.2147/IJN.S29629
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