Cargando…
Increased Levels of Human Carotid Lesion Linoleic Acid Hydroperoxide in Symptomatic and Asymptomatic Patients Is Inversely Correlated with Serum HDL and Paraoxonase 1 Activity
Human carotid plaque components interact directly with circulating blood elements and thus they might affect each other. We determined plaque paraoxonase1 (PON1) hydrolytic-catalytic activity and compared plaque and blood levels of lipids, HDL, PON1, and HbA1c, as well as plaque-oxidized lipids in s...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3368548/ https://www.ncbi.nlm.nih.gov/pubmed/22690338 http://dx.doi.org/10.1155/2012/762560 |
_version_ | 1782234967413096448 |
---|---|
author | Cohen, Elad Aviram, Michael Khatib, Soliman Rabin, Asaf Mannheim, Dalit Karmeli, Ron Vaya, Jacob |
author_facet | Cohen, Elad Aviram, Michael Khatib, Soliman Rabin, Asaf Mannheim, Dalit Karmeli, Ron Vaya, Jacob |
author_sort | Cohen, Elad |
collection | PubMed |
description | Human carotid plaque components interact directly with circulating blood elements and thus they might affect each other. We determined plaque paraoxonase1 (PON1) hydrolytic-catalytic activity and compared plaque and blood levels of lipids, HDL, PON1, and HbA1c, as well as plaque-oxidized lipids in symptomatic and asymptomatic patients. Human carotid plaques were obtained from symptomatic and asymptomatic patients undergoing routine endarterectomy, and the lesions were ground and extracted for PON activity and lipid content determinations. Plaque PONs preserved paraoxonase, arylesterase, and lactonase activities. The PON1-specific inhibitor 2-hydroxyquinoline almost completely inhibited paraoxonase and lactonase activities, while only moderately inhibiting arylesterase activity. Oxysterol and triglyceride levels in plaques from symptomatic and asymptomatic patients did not differ significantly, but plaques from symptomatic patients had significantly higher (135%) linoleic acid hydroperoxide (LA-13OOH) levels. Their serum PON1 activity, cholesterol and triglyceride levels did not differ significantly, but symptomatic patients had significantly lower (28%) serum HDL levels and higher (18%) HbA1c levels. Thus LA-13OOH, a major atherogenic plaque element, showed significant negative correlations with serum PON1 activity and HDL levels, and a positive correlation with the prodiabetic atherogenic HbA1c. Plaque PON1 retains its activity and may decrease plaque atherogenicity by reducing specific oxidized lipids (e.g., LA-13OOH). The inverse correlation between plaque LA-13OOH level and serum HDL level and PON1 activity suggests a role for serum HDL and PON1 in LA-13OOH accumulation. |
format | Online Article Text |
id | pubmed-3368548 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-33685482012-06-11 Increased Levels of Human Carotid Lesion Linoleic Acid Hydroperoxide in Symptomatic and Asymptomatic Patients Is Inversely Correlated with Serum HDL and Paraoxonase 1 Activity Cohen, Elad Aviram, Michael Khatib, Soliman Rabin, Asaf Mannheim, Dalit Karmeli, Ron Vaya, Jacob J Lipids Research Article Human carotid plaque components interact directly with circulating blood elements and thus they might affect each other. We determined plaque paraoxonase1 (PON1) hydrolytic-catalytic activity and compared plaque and blood levels of lipids, HDL, PON1, and HbA1c, as well as plaque-oxidized lipids in symptomatic and asymptomatic patients. Human carotid plaques were obtained from symptomatic and asymptomatic patients undergoing routine endarterectomy, and the lesions were ground and extracted for PON activity and lipid content determinations. Plaque PONs preserved paraoxonase, arylesterase, and lactonase activities. The PON1-specific inhibitor 2-hydroxyquinoline almost completely inhibited paraoxonase and lactonase activities, while only moderately inhibiting arylesterase activity. Oxysterol and triglyceride levels in plaques from symptomatic and asymptomatic patients did not differ significantly, but plaques from symptomatic patients had significantly higher (135%) linoleic acid hydroperoxide (LA-13OOH) levels. Their serum PON1 activity, cholesterol and triglyceride levels did not differ significantly, but symptomatic patients had significantly lower (28%) serum HDL levels and higher (18%) HbA1c levels. Thus LA-13OOH, a major atherogenic plaque element, showed significant negative correlations with serum PON1 activity and HDL levels, and a positive correlation with the prodiabetic atherogenic HbA1c. Plaque PON1 retains its activity and may decrease plaque atherogenicity by reducing specific oxidized lipids (e.g., LA-13OOH). The inverse correlation between plaque LA-13OOH level and serum HDL level and PON1 activity suggests a role for serum HDL and PON1 in LA-13OOH accumulation. Hindawi Publishing Corporation 2012 2012-05-29 /pmc/articles/PMC3368548/ /pubmed/22690338 http://dx.doi.org/10.1155/2012/762560 Text en Copyright © 2012 Elad Cohen et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Cohen, Elad Aviram, Michael Khatib, Soliman Rabin, Asaf Mannheim, Dalit Karmeli, Ron Vaya, Jacob Increased Levels of Human Carotid Lesion Linoleic Acid Hydroperoxide in Symptomatic and Asymptomatic Patients Is Inversely Correlated with Serum HDL and Paraoxonase 1 Activity |
title | Increased Levels of Human Carotid Lesion Linoleic Acid Hydroperoxide in Symptomatic and Asymptomatic Patients Is Inversely Correlated with Serum HDL and Paraoxonase 1 Activity |
title_full | Increased Levels of Human Carotid Lesion Linoleic Acid Hydroperoxide in Symptomatic and Asymptomatic Patients Is Inversely Correlated with Serum HDL and Paraoxonase 1 Activity |
title_fullStr | Increased Levels of Human Carotid Lesion Linoleic Acid Hydroperoxide in Symptomatic and Asymptomatic Patients Is Inversely Correlated with Serum HDL and Paraoxonase 1 Activity |
title_full_unstemmed | Increased Levels of Human Carotid Lesion Linoleic Acid Hydroperoxide in Symptomatic and Asymptomatic Patients Is Inversely Correlated with Serum HDL and Paraoxonase 1 Activity |
title_short | Increased Levels of Human Carotid Lesion Linoleic Acid Hydroperoxide in Symptomatic and Asymptomatic Patients Is Inversely Correlated with Serum HDL and Paraoxonase 1 Activity |
title_sort | increased levels of human carotid lesion linoleic acid hydroperoxide in symptomatic and asymptomatic patients is inversely correlated with serum hdl and paraoxonase 1 activity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3368548/ https://www.ncbi.nlm.nih.gov/pubmed/22690338 http://dx.doi.org/10.1155/2012/762560 |
work_keys_str_mv | AT cohenelad increasedlevelsofhumancarotidlesionlinoleicacidhydroperoxideinsymptomaticandasymptomaticpatientsisinverselycorrelatedwithserumhdlandparaoxonase1activity AT avirammichael increasedlevelsofhumancarotidlesionlinoleicacidhydroperoxideinsymptomaticandasymptomaticpatientsisinverselycorrelatedwithserumhdlandparaoxonase1activity AT khatibsoliman increasedlevelsofhumancarotidlesionlinoleicacidhydroperoxideinsymptomaticandasymptomaticpatientsisinverselycorrelatedwithserumhdlandparaoxonase1activity AT rabinasaf increasedlevelsofhumancarotidlesionlinoleicacidhydroperoxideinsymptomaticandasymptomaticpatientsisinverselycorrelatedwithserumhdlandparaoxonase1activity AT mannheimdalit increasedlevelsofhumancarotidlesionlinoleicacidhydroperoxideinsymptomaticandasymptomaticpatientsisinverselycorrelatedwithserumhdlandparaoxonase1activity AT karmeliron increasedlevelsofhumancarotidlesionlinoleicacidhydroperoxideinsymptomaticandasymptomaticpatientsisinverselycorrelatedwithserumhdlandparaoxonase1activity AT vayajacob increasedlevelsofhumancarotidlesionlinoleicacidhydroperoxideinsymptomaticandasymptomaticpatientsisinverselycorrelatedwithserumhdlandparaoxonase1activity |