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Dynamics of Endoreplication during Drosophila Posterior Scutellar Macrochaete Development

Endoreplication is a variant type of DNA replication, consisting only of alternating G1 and S phases. Many types of Drosophila tissues undergo endoreplication. However, the timing and the extent to which a single endocycling macrochaete undergoes temporally programmed endoreplication during developm...

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Autores principales: Kawamori, Akihito, Shimaji, Kouhei, Yamaguchi, Masamitsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3368872/
https://www.ncbi.nlm.nih.gov/pubmed/22701699
http://dx.doi.org/10.1371/journal.pone.0038714
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author Kawamori, Akihito
Shimaji, Kouhei
Yamaguchi, Masamitsu
author_facet Kawamori, Akihito
Shimaji, Kouhei
Yamaguchi, Masamitsu
author_sort Kawamori, Akihito
collection PubMed
description Endoreplication is a variant type of DNA replication, consisting only of alternating G1 and S phases. Many types of Drosophila tissues undergo endoreplication. However, the timing and the extent to which a single endocycling macrochaete undergoes temporally programmed endoreplication during development are unclear. Here, we focused on the dynamics of endoreplication during posterior scutellar (pSC) macrochaete development. Quantitative analyses of C values in shaft cells and socket cells revealed a gradual rise from 8C and 4C at 8 hours after pupal formation (APF) to 72C and 24C at 29 hours APF, respectively. The validity of the values was further confirmed by the measurement of DNA content with a confocal laser microscope. BrdU incorporation assays demonstrated that shaft cells undergo four rounds of endoreplication from 18 to 29.5 hours APF. In contrast, socket cells undergo two rounds of endoreplication during the same period. Statistical analyses showed that the theoretical C values, based on BrdU assays, nearly coincide with the actually measured C values in socket cells, but not in shaft cells after 22 hours APF. These analyses suggest that socket cells undergo two rounds of endoreplication. However, the mechanism of endoreplication in the shaft cells may change from 22 hours APF, suggesting the possibility that shaft cells undergo two or four rounds of endoreplication during the periods. We also found that the timing of endoreplication differs, depending on the type of macrochaete. Moreover, endocycling in shaft cells of both the left and right sides of pSC bristle lineages occurs in the same pattern, indicating that the process is synchronized for specific types of macrochaete. Our findings suggest that endocycling in macrochaete cell lineages can be a model for understanding mechanisms of endoreplication at the single-cell level.
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spelling pubmed-33688722012-06-13 Dynamics of Endoreplication during Drosophila Posterior Scutellar Macrochaete Development Kawamori, Akihito Shimaji, Kouhei Yamaguchi, Masamitsu PLoS One Research Article Endoreplication is a variant type of DNA replication, consisting only of alternating G1 and S phases. Many types of Drosophila tissues undergo endoreplication. However, the timing and the extent to which a single endocycling macrochaete undergoes temporally programmed endoreplication during development are unclear. Here, we focused on the dynamics of endoreplication during posterior scutellar (pSC) macrochaete development. Quantitative analyses of C values in shaft cells and socket cells revealed a gradual rise from 8C and 4C at 8 hours after pupal formation (APF) to 72C and 24C at 29 hours APF, respectively. The validity of the values was further confirmed by the measurement of DNA content with a confocal laser microscope. BrdU incorporation assays demonstrated that shaft cells undergo four rounds of endoreplication from 18 to 29.5 hours APF. In contrast, socket cells undergo two rounds of endoreplication during the same period. Statistical analyses showed that the theoretical C values, based on BrdU assays, nearly coincide with the actually measured C values in socket cells, but not in shaft cells after 22 hours APF. These analyses suggest that socket cells undergo two rounds of endoreplication. However, the mechanism of endoreplication in the shaft cells may change from 22 hours APF, suggesting the possibility that shaft cells undergo two or four rounds of endoreplication during the periods. We also found that the timing of endoreplication differs, depending on the type of macrochaete. Moreover, endocycling in shaft cells of both the left and right sides of pSC bristle lineages occurs in the same pattern, indicating that the process is synchronized for specific types of macrochaete. Our findings suggest that endocycling in macrochaete cell lineages can be a model for understanding mechanisms of endoreplication at the single-cell level. Public Library of Science 2012-06-06 /pmc/articles/PMC3368872/ /pubmed/22701699 http://dx.doi.org/10.1371/journal.pone.0038714 Text en Kawamori et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kawamori, Akihito
Shimaji, Kouhei
Yamaguchi, Masamitsu
Dynamics of Endoreplication during Drosophila Posterior Scutellar Macrochaete Development
title Dynamics of Endoreplication during Drosophila Posterior Scutellar Macrochaete Development
title_full Dynamics of Endoreplication during Drosophila Posterior Scutellar Macrochaete Development
title_fullStr Dynamics of Endoreplication during Drosophila Posterior Scutellar Macrochaete Development
title_full_unstemmed Dynamics of Endoreplication during Drosophila Posterior Scutellar Macrochaete Development
title_short Dynamics of Endoreplication during Drosophila Posterior Scutellar Macrochaete Development
title_sort dynamics of endoreplication during drosophila posterior scutellar macrochaete development
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3368872/
https://www.ncbi.nlm.nih.gov/pubmed/22701699
http://dx.doi.org/10.1371/journal.pone.0038714
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