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Association between polymorphism in the FTO gene and growth and carcass traits in pig crosses

BACKGROUND: Independent studies have shown that several single nucleotide polymorphisms (SNP) in the human FTO (fat mass and obesity associated) gene are associated with obesity. SNP have also been identified in the pig FTO gene, among which some are associated with selected fat-deposition traits in...

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Autores principales: Dvořáková, Věra, Bartenschlager, Heinz, Stratil, Antonín, Horák, Pavel, Stupka, Roman, Čítek, Jaroslav, Šprysl, Michal, Hrdlicová, Anna, Geldermann, Hermann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3369214/
https://www.ncbi.nlm.nih.gov/pubmed/22510482
http://dx.doi.org/10.1186/1297-9686-44-13
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author Dvořáková, Věra
Bartenschlager, Heinz
Stratil, Antonín
Horák, Pavel
Stupka, Roman
Čítek, Jaroslav
Šprysl, Michal
Hrdlicová, Anna
Geldermann, Hermann
author_facet Dvořáková, Věra
Bartenschlager, Heinz
Stratil, Antonín
Horák, Pavel
Stupka, Roman
Čítek, Jaroslav
Šprysl, Michal
Hrdlicová, Anna
Geldermann, Hermann
author_sort Dvořáková, Věra
collection PubMed
description BACKGROUND: Independent studies have shown that several single nucleotide polymorphisms (SNP) in the human FTO (fat mass and obesity associated) gene are associated with obesity. SNP have also been identified in the pig FTO gene, among which some are associated with selected fat-deposition traits in F(2 )crosses and commercial populations. In this study, using both commercial pig populations and an experimental Meishan × Pietrain F(2 )population, we have investigated the association between one FTO SNP and several growth and carcass traits. Association analyses were performed with the FTO polymorphism either alone or in combination with polymorphisms in flanking loci. METHODS: SNP (FM244720:g.400C>G) in exon 3 of porcine FTO was genotyped by PCR-RFLP and tested for associations with some growth, carcass and fat-related traits. Proportions of genetic variance of four pig chromosome 6 genes (FTO, RYR1, LIPE and TGFB1) on selected traits were evaluated using single- and multi-locus models. RESULTS: Linkage analysis placed FTO on the p arm of pig chromosome 6, approximately 22 cM from RYR1. In the commercial populations, allele C of the FTO SNP was significantly associated with back fat depth and allele G with muscling traits. In the Meishan × Pietrain F(2 )pigs, heterozygotes with allele C from the Pietrain sows and allele G from the Meishan boar were more significantly associated with fat-related traits compared to homozygotes with allele G from the Pietrain and allele G from the Meishan breed. In single- and multi-locus models, genes RYR1, TGFB1 and FTO showed high associations. The contribution in genetic variance from the polymorphism in the FTO gene was highest for back fat depth, meat area on the musculus longissimus lumborum et thoracis tissues and metabolite glucose-6-phosphate dehydrogenase. CONCLUSIONS: Our results show that in pig, FTO influences back fat depth in the commercial populations, while in the Meishan × Pietrain F(2 )pigs with a CG genotype, heterosis occurs for several fat-related traits.
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spelling pubmed-33692142012-06-07 Association between polymorphism in the FTO gene and growth and carcass traits in pig crosses Dvořáková, Věra Bartenschlager, Heinz Stratil, Antonín Horák, Pavel Stupka, Roman Čítek, Jaroslav Šprysl, Michal Hrdlicová, Anna Geldermann, Hermann Genet Sel Evol Research BACKGROUND: Independent studies have shown that several single nucleotide polymorphisms (SNP) in the human FTO (fat mass and obesity associated) gene are associated with obesity. SNP have also been identified in the pig FTO gene, among which some are associated with selected fat-deposition traits in F(2 )crosses and commercial populations. In this study, using both commercial pig populations and an experimental Meishan × Pietrain F(2 )population, we have investigated the association between one FTO SNP and several growth and carcass traits. Association analyses were performed with the FTO polymorphism either alone or in combination with polymorphisms in flanking loci. METHODS: SNP (FM244720:g.400C>G) in exon 3 of porcine FTO was genotyped by PCR-RFLP and tested for associations with some growth, carcass and fat-related traits. Proportions of genetic variance of four pig chromosome 6 genes (FTO, RYR1, LIPE and TGFB1) on selected traits were evaluated using single- and multi-locus models. RESULTS: Linkage analysis placed FTO on the p arm of pig chromosome 6, approximately 22 cM from RYR1. In the commercial populations, allele C of the FTO SNP was significantly associated with back fat depth and allele G with muscling traits. In the Meishan × Pietrain F(2 )pigs, heterozygotes with allele C from the Pietrain sows and allele G from the Meishan boar were more significantly associated with fat-related traits compared to homozygotes with allele G from the Pietrain and allele G from the Meishan breed. In single- and multi-locus models, genes RYR1, TGFB1 and FTO showed high associations. The contribution in genetic variance from the polymorphism in the FTO gene was highest for back fat depth, meat area on the musculus longissimus lumborum et thoracis tissues and metabolite glucose-6-phosphate dehydrogenase. CONCLUSIONS: Our results show that in pig, FTO influences back fat depth in the commercial populations, while in the Meishan × Pietrain F(2 )pigs with a CG genotype, heterosis occurs for several fat-related traits. BioMed Central 2012-04-17 /pmc/articles/PMC3369214/ /pubmed/22510482 http://dx.doi.org/10.1186/1297-9686-44-13 Text en Copyright ©2012 Dvořáková et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Dvořáková, Věra
Bartenschlager, Heinz
Stratil, Antonín
Horák, Pavel
Stupka, Roman
Čítek, Jaroslav
Šprysl, Michal
Hrdlicová, Anna
Geldermann, Hermann
Association between polymorphism in the FTO gene and growth and carcass traits in pig crosses
title Association between polymorphism in the FTO gene and growth and carcass traits in pig crosses
title_full Association between polymorphism in the FTO gene and growth and carcass traits in pig crosses
title_fullStr Association between polymorphism in the FTO gene and growth and carcass traits in pig crosses
title_full_unstemmed Association between polymorphism in the FTO gene and growth and carcass traits in pig crosses
title_short Association between polymorphism in the FTO gene and growth and carcass traits in pig crosses
title_sort association between polymorphism in the fto gene and growth and carcass traits in pig crosses
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3369214/
https://www.ncbi.nlm.nih.gov/pubmed/22510482
http://dx.doi.org/10.1186/1297-9686-44-13
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