Image patterned molecular delivery into live cells using gold particle coated substrates

An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer. Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified b...

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Detalles Bibliográficos
Autores principales: Wu, Ting-Hsiang, Kalim, Sheraz, Callahan, Caitlin, Teitell, Michael A., Chiou, Pei-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2010
Materias:
Research-Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3369548/
https://www.ncbi.nlm.nih.gov/pubmed/20173916
http://dx.doi.org/10.1364/OE.18.000938
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author Wu, Ting-Hsiang
Kalim, Sheraz
Callahan, Caitlin
Teitell, Michael A.
Chiou, Pei-Yu
author_facet Wu, Ting-Hsiang
Kalim, Sheraz
Callahan, Caitlin
Teitell, Michael A.
Chiou, Pei-Yu
author_sort Wu, Ting-Hsiang
collection PubMed
description An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer. Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein. Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.
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spelling pubmed-33695482012-10-01 Image patterned molecular delivery into live cells using gold particle coated substrates Wu, Ting-Hsiang Kalim, Sheraz Callahan, Caitlin Teitell, Michael A. Chiou, Pei-Yu Opt Express Research-Article An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer. Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein. Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated. Optical Society of America 2010-01-07 /pmc/articles/PMC3369548/ /pubmed/20173916 http://dx.doi.org/10.1364/OE.18.000938 Text en ©2010 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Research-Article
Wu, Ting-Hsiang
Kalim, Sheraz
Callahan, Caitlin
Teitell, Michael A.
Chiou, Pei-Yu
Image patterned molecular delivery into live cells using gold particle coated substrates
title Image patterned molecular delivery into live cells using gold particle coated substrates
title_full Image patterned molecular delivery into live cells using gold particle coated substrates
title_fullStr Image patterned molecular delivery into live cells using gold particle coated substrates
title_full_unstemmed Image patterned molecular delivery into live cells using gold particle coated substrates
title_short Image patterned molecular delivery into live cells using gold particle coated substrates
title_sort image patterned molecular delivery into live cells using gold particle coated substrates
topic Research-Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3369548/
https://www.ncbi.nlm.nih.gov/pubmed/20173916
http://dx.doi.org/10.1364/OE.18.000938
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AT teitellmichaela imagepatternedmoleculardeliveryintolivecellsusinggoldparticlecoatedsubstrates
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