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Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence

We describe a new method for imaging leukocytes in vivo by exciting the endogenous protein fluorescence in the ultraviolet (UV) spectral region where tryptophan is the major fluorophore. Two-photon excitation near 590 nm allows noninvasive optical sectioning through the epidermal cell layers into th...

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Autores principales: Li, Chunqiang, Pastila, Riikka K., Pitsillides, Costas, Runnels, Judith M., Puoris’haag, Mehron, Côté, Daniel, Lin, Charles P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3369551/
https://www.ncbi.nlm.nih.gov/pubmed/20173920
http://dx.doi.org/10.1364/OE.18.000988
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author Li, Chunqiang
Pastila, Riikka K.
Pitsillides, Costas
Runnels, Judith M.
Puoris’haag, Mehron
Côté, Daniel
Lin, Charles P.
author_facet Li, Chunqiang
Pastila, Riikka K.
Pitsillides, Costas
Runnels, Judith M.
Puoris’haag, Mehron
Côté, Daniel
Lin, Charles P.
author_sort Li, Chunqiang
collection PubMed
description We describe a new method for imaging leukocytes in vivo by exciting the endogenous protein fluorescence in the ultraviolet (UV) spectral region where tryptophan is the major fluorophore. Two-photon excitation near 590 nm allows noninvasive optical sectioning through the epidermal cell layers into the dermis of mouse skin, where leukocytes can be observed by video-rate microscopy to interact dynamically with the dermal vascular endothelium. Inflammation significantly enhances leukocyte rolling, adhesion, and tissue infiltration. After exiting the vasculature, leukocytes continue to move actively in tissue as observed by time-lapse microscopy, and are distinguishable from resident autofluorescent cells that are not motile. Because the new method alleviates the need to introduce exogenous labels, it is potentially applicable for tracking leukocytes and monitoring inflammatory cellular reactions in humans.
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spelling pubmed-33695512012-10-01 Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence Li, Chunqiang Pastila, Riikka K. Pitsillides, Costas Runnels, Judith M. Puoris’haag, Mehron Côté, Daniel Lin, Charles P. Opt Express Research-Article We describe a new method for imaging leukocytes in vivo by exciting the endogenous protein fluorescence in the ultraviolet (UV) spectral region where tryptophan is the major fluorophore. Two-photon excitation near 590 nm allows noninvasive optical sectioning through the epidermal cell layers into the dermis of mouse skin, where leukocytes can be observed by video-rate microscopy to interact dynamically with the dermal vascular endothelium. Inflammation significantly enhances leukocyte rolling, adhesion, and tissue infiltration. After exiting the vasculature, leukocytes continue to move actively in tissue as observed by time-lapse microscopy, and are distinguishable from resident autofluorescent cells that are not motile. Because the new method alleviates the need to introduce exogenous labels, it is potentially applicable for tracking leukocytes and monitoring inflammatory cellular reactions in humans. Optical Society of America 2010-01-07 /pmc/articles/PMC3369551/ /pubmed/20173920 http://dx.doi.org/10.1364/OE.18.000988 Text en ©2010 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Research-Article
Li, Chunqiang
Pastila, Riikka K.
Pitsillides, Costas
Runnels, Judith M.
Puoris’haag, Mehron
Côté, Daniel
Lin, Charles P.
Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence
title Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence
title_full Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence
title_fullStr Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence
title_full_unstemmed Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence
title_short Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence
title_sort imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence
topic Research-Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3369551/
https://www.ncbi.nlm.nih.gov/pubmed/20173920
http://dx.doi.org/10.1364/OE.18.000988
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