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Interferon-gamma and interleukin-10 production by mononuclear cells from patients with advanced head and neck cancer

OBJECTIVE: This study aims to evaluate the production of interferon-gamma and interleukin-10 by stimulated peripheral blood mononuclear cells isolated from patients with supraglottic laryngeal cancer before and after surgical treatment. METHODS: Fourteen patients with advanced supraglottic laryngeal...

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Autores principales: Conti-Freitas, Luiz C, Foss-Freitas, Maria C, Mamede, Rui C M, Foss, Norma T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3370309/
https://www.ncbi.nlm.nih.gov/pubmed/22760896
http://dx.doi.org/10.6061/clinics/2012(06)07
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author Conti-Freitas, Luiz C
Foss-Freitas, Maria C
Mamede, Rui C M
Foss, Norma T
author_facet Conti-Freitas, Luiz C
Foss-Freitas, Maria C
Mamede, Rui C M
Foss, Norma T
author_sort Conti-Freitas, Luiz C
collection PubMed
description OBJECTIVE: This study aims to evaluate the production of interferon-gamma and interleukin-10 by stimulated peripheral blood mononuclear cells isolated from patients with supraglottic laryngeal cancer before and after surgical treatment. METHODS: Fourteen patients with advanced supraglottic laryngeal cancer were studied. Cultures of peripheral blood mononuclear cells isolated during the preoperative and late postoperative periods were stimulated with concanavalin A and Bacille Calmette-Guérin, and the supernatant concentrations of interferon-gamma and interleukin-10 were measured. RESULTS: For non-stimulated cultures, the interferon-gamma levels produced by the preoperative period and the late postoperative period cultures were lower than the levels produced by the control group cultures. The interferon-gamma levels after stimulation with concanavalin A were higher in the late postoperative period cultures than in the preoperative evaluation cultures. Stimulation with Bacille Calmette-Guérin led to the production of similar levels of interferon-gamma and interleukin-10 by all cultures; thus, stimulation increased the levels of interferon-gamma produced by both the preoperative and postoperative cultures relative to the levels produced by the corresponding unstimulated cultures. CONCLUSION: Patients with advanced supraglottic laryngeal cancer exhibit an in vitro deficiency in interferon-gamma secretion by mononuclear cells. Stimulated cells seem to recover this function during the postoperative period.
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spelling pubmed-33703092012-06-11 Interferon-gamma and interleukin-10 production by mononuclear cells from patients with advanced head and neck cancer Conti-Freitas, Luiz C Foss-Freitas, Maria C Mamede, Rui C M Foss, Norma T Clinics (Sao Paulo) Clinical Science OBJECTIVE: This study aims to evaluate the production of interferon-gamma and interleukin-10 by stimulated peripheral blood mononuclear cells isolated from patients with supraglottic laryngeal cancer before and after surgical treatment. METHODS: Fourteen patients with advanced supraglottic laryngeal cancer were studied. Cultures of peripheral blood mononuclear cells isolated during the preoperative and late postoperative periods were stimulated with concanavalin A and Bacille Calmette-Guérin, and the supernatant concentrations of interferon-gamma and interleukin-10 were measured. RESULTS: For non-stimulated cultures, the interferon-gamma levels produced by the preoperative period and the late postoperative period cultures were lower than the levels produced by the control group cultures. The interferon-gamma levels after stimulation with concanavalin A were higher in the late postoperative period cultures than in the preoperative evaluation cultures. Stimulation with Bacille Calmette-Guérin led to the production of similar levels of interferon-gamma and interleukin-10 by all cultures; thus, stimulation increased the levels of interferon-gamma produced by both the preoperative and postoperative cultures relative to the levels produced by the corresponding unstimulated cultures. CONCLUSION: Patients with advanced supraglottic laryngeal cancer exhibit an in vitro deficiency in interferon-gamma secretion by mononuclear cells. Stimulated cells seem to recover this function during the postoperative period. Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2012-06 /pmc/articles/PMC3370309/ /pubmed/22760896 http://dx.doi.org/10.6061/clinics/2012(06)07 Text en Copyright © 2012 Hospital das Clínicas da FMUSP http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Clinical Science
Conti-Freitas, Luiz C
Foss-Freitas, Maria C
Mamede, Rui C M
Foss, Norma T
Interferon-gamma and interleukin-10 production by mononuclear cells from patients with advanced head and neck cancer
title Interferon-gamma and interleukin-10 production by mononuclear cells from patients with advanced head and neck cancer
title_full Interferon-gamma and interleukin-10 production by mononuclear cells from patients with advanced head and neck cancer
title_fullStr Interferon-gamma and interleukin-10 production by mononuclear cells from patients with advanced head and neck cancer
title_full_unstemmed Interferon-gamma and interleukin-10 production by mononuclear cells from patients with advanced head and neck cancer
title_short Interferon-gamma and interleukin-10 production by mononuclear cells from patients with advanced head and neck cancer
title_sort interferon-gamma and interleukin-10 production by mononuclear cells from patients with advanced head and neck cancer
topic Clinical Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3370309/
https://www.ncbi.nlm.nih.gov/pubmed/22760896
http://dx.doi.org/10.6061/clinics/2012(06)07
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