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Circulating Cytokines and Nitric Oxide are Involved in the Inhibition of Neutrophil Migration in Patients with Uterine Cervical Neoplasia
AIM: To verify if patients with cervical neoplasia produce mediators that reduce leukocyte function. METHODS: Control neutrophils incubated with normal serum or serum from pre-invasive or invasive neoplasia patients were assayed for chemotaxis. Mediators were assayed in serum and in leukocyte supern...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Libertas Academica
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3370832/ https://www.ncbi.nlm.nih.gov/pubmed/22693424 http://dx.doi.org/10.4137/CMO.S9518 |
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author | Micheli, Douglas Côbo Fernandes, Paulo Cesar Cruvinel, João Celso Garcia Nomelini, Isabela Destro Murta, Eddie Fernando Candido Tavares-Murta, Beatriz Martins |
author_facet | Micheli, Douglas Côbo Fernandes, Paulo Cesar Cruvinel, João Celso Garcia Nomelini, Isabela Destro Murta, Eddie Fernando Candido Tavares-Murta, Beatriz Martins |
author_sort | Micheli, Douglas Côbo |
collection | PubMed |
description | AIM: To verify if patients with cervical neoplasia produce mediators that reduce leukocyte function. METHODS: Control neutrophils incubated with normal serum or serum from pre-invasive or invasive neoplasia patients were assayed for chemotaxis. Mediators were assayed in serum and in leukocyte supernatants. Experiments were also performed in random patients after surgery. RESULTS: Neutrophils incubated with patient sera, but not normal sera, failed to migrate towards the chemoattractants. In invasive neoplasia compared to controls, IL-6 and IL-8, and IL-10 and TNF-α were elevated in serum and in neutrophil supernatants, respectively. Nitrite levels were elevated in mononuclear cell supernatants from patients than controls. After surgery, serum cytokine levels were reduced, mainly in pre-invasive patients. Neutrophils treated with serum from pre-invasive patients undergone surgery had restored migration. CONCLUSION: Patients with cervical neoplasia produce mediators, predominantly induced by tumor cells, able to impair the inflammatory response at very early stages of disease. |
format | Online Article Text |
id | pubmed-3370832 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Libertas Academica |
record_format | MEDLINE/PubMed |
spelling | pubmed-33708322012-06-12 Circulating Cytokines and Nitric Oxide are Involved in the Inhibition of Neutrophil Migration in Patients with Uterine Cervical Neoplasia Micheli, Douglas Côbo Fernandes, Paulo Cesar Cruvinel, João Celso Garcia Nomelini, Isabela Destro Murta, Eddie Fernando Candido Tavares-Murta, Beatriz Martins Clin Med Insights Oncol Original Research AIM: To verify if patients with cervical neoplasia produce mediators that reduce leukocyte function. METHODS: Control neutrophils incubated with normal serum or serum from pre-invasive or invasive neoplasia patients were assayed for chemotaxis. Mediators were assayed in serum and in leukocyte supernatants. Experiments were also performed in random patients after surgery. RESULTS: Neutrophils incubated with patient sera, but not normal sera, failed to migrate towards the chemoattractants. In invasive neoplasia compared to controls, IL-6 and IL-8, and IL-10 and TNF-α were elevated in serum and in neutrophil supernatants, respectively. Nitrite levels were elevated in mononuclear cell supernatants from patients than controls. After surgery, serum cytokine levels were reduced, mainly in pre-invasive patients. Neutrophils treated with serum from pre-invasive patients undergone surgery had restored migration. CONCLUSION: Patients with cervical neoplasia produce mediators, predominantly induced by tumor cells, able to impair the inflammatory response at very early stages of disease. Libertas Academica 2012-05-28 /pmc/articles/PMC3370832/ /pubmed/22693424 http://dx.doi.org/10.4137/CMO.S9518 Text en © the author(s), publisher and licensee Libertas Academica Ltd. This is an open access article. Unrestricted non-commercial use is permitted provided the original work is properly cited. |
spellingShingle | Original Research Micheli, Douglas Côbo Fernandes, Paulo Cesar Cruvinel, João Celso Garcia Nomelini, Isabela Destro Murta, Eddie Fernando Candido Tavares-Murta, Beatriz Martins Circulating Cytokines and Nitric Oxide are Involved in the Inhibition of Neutrophil Migration in Patients with Uterine Cervical Neoplasia |
title | Circulating Cytokines and Nitric Oxide are Involved in the Inhibition of Neutrophil Migration in Patients with Uterine Cervical Neoplasia |
title_full | Circulating Cytokines and Nitric Oxide are Involved in the Inhibition of Neutrophil Migration in Patients with Uterine Cervical Neoplasia |
title_fullStr | Circulating Cytokines and Nitric Oxide are Involved in the Inhibition of Neutrophil Migration in Patients with Uterine Cervical Neoplasia |
title_full_unstemmed | Circulating Cytokines and Nitric Oxide are Involved in the Inhibition of Neutrophil Migration in Patients with Uterine Cervical Neoplasia |
title_short | Circulating Cytokines and Nitric Oxide are Involved in the Inhibition of Neutrophil Migration in Patients with Uterine Cervical Neoplasia |
title_sort | circulating cytokines and nitric oxide are involved in the inhibition of neutrophil migration in patients with uterine cervical neoplasia |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3370832/ https://www.ncbi.nlm.nih.gov/pubmed/22693424 http://dx.doi.org/10.4137/CMO.S9518 |
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