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Broadband ultraviolet-visible optical property measurement in layered turbid media

The ability to accurately measure layered biological tissue optical properties (OPs) may improve understanding of spectroscopic device performance and facilitate early cancer detection. Towards these goals, we have performed theoretical and experimental evaluations of an approach for broadband measu...

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Detalles Bibliográficos
Autores principales: Wang, Quanzeng, Le, Du, Ramella-Roman, Jessica, Pfefer, Joshua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3370964/
https://www.ncbi.nlm.nih.gov/pubmed/22741070
http://dx.doi.org/10.1364/BOE.3.001226
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author Wang, Quanzeng
Le, Du
Ramella-Roman, Jessica
Pfefer, Joshua
author_facet Wang, Quanzeng
Le, Du
Ramella-Roman, Jessica
Pfefer, Joshua
author_sort Wang, Quanzeng
collection PubMed
description The ability to accurately measure layered biological tissue optical properties (OPs) may improve understanding of spectroscopic device performance and facilitate early cancer detection. Towards these goals, we have performed theoretical and experimental evaluations of an approach for broadband measurement of absorption and reduced scattering coefficients at ultraviolet-visible wavelengths. Our technique is based on neural network (NN) inverse models trained with diffuse reflectance data from condensed Monte Carlo simulations. Experimental measurements were performed from 350 to 600 nm with a fiber-optic-based reflectance spectroscopy system. Two-layer phantoms incorporating OPs relevant to normal and dysplastic mucosal tissue and superficial layer thicknesses of 0.22 and 0.44 mm were used to assess prediction accuracy. Results showed mean OP estimation errors of 19% from the theoretical analysis and 27% from experiments. Two-step NN modeling and nonlinear spectral fitting approaches helped improve prediction accuracy. While limitations and challenges remain, the results of this study indicate that our technique can provide moderately accurate estimates of OPs in layered turbid media.
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spelling pubmed-33709642012-06-27 Broadband ultraviolet-visible optical property measurement in layered turbid media Wang, Quanzeng Le, Du Ramella-Roman, Jessica Pfefer, Joshua Biomed Opt Express Calibration, Validation and Phantom Studies The ability to accurately measure layered biological tissue optical properties (OPs) may improve understanding of spectroscopic device performance and facilitate early cancer detection. Towards these goals, we have performed theoretical and experimental evaluations of an approach for broadband measurement of absorption and reduced scattering coefficients at ultraviolet-visible wavelengths. Our technique is based on neural network (NN) inverse models trained with diffuse reflectance data from condensed Monte Carlo simulations. Experimental measurements were performed from 350 to 600 nm with a fiber-optic-based reflectance spectroscopy system. Two-layer phantoms incorporating OPs relevant to normal and dysplastic mucosal tissue and superficial layer thicknesses of 0.22 and 0.44 mm were used to assess prediction accuracy. Results showed mean OP estimation errors of 19% from the theoretical analysis and 27% from experiments. Two-step NN modeling and nonlinear spectral fitting approaches helped improve prediction accuracy. While limitations and challenges remain, the results of this study indicate that our technique can provide moderately accurate estimates of OPs in layered turbid media. Optical Society of America 2012-05-03 /pmc/articles/PMC3370964/ /pubmed/22741070 http://dx.doi.org/10.1364/BOE.3.001226 Text en ©2012 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Calibration, Validation and Phantom Studies
Wang, Quanzeng
Le, Du
Ramella-Roman, Jessica
Pfefer, Joshua
Broadband ultraviolet-visible optical property measurement in layered turbid media
title Broadband ultraviolet-visible optical property measurement in layered turbid media
title_full Broadband ultraviolet-visible optical property measurement in layered turbid media
title_fullStr Broadband ultraviolet-visible optical property measurement in layered turbid media
title_full_unstemmed Broadband ultraviolet-visible optical property measurement in layered turbid media
title_short Broadband ultraviolet-visible optical property measurement in layered turbid media
title_sort broadband ultraviolet-visible optical property measurement in layered turbid media
topic Calibration, Validation and Phantom Studies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3370964/
https://www.ncbi.nlm.nih.gov/pubmed/22741070
http://dx.doi.org/10.1364/BOE.3.001226
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