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In situ three-dimensional monitoring of collagen fibrillogenesis using SHG microscopy
We implemented in situ time-lapse Second Harmonic Generation (SHG) microscopy to monitor the three-dimensional (3D) self-assembly of collagen in solution. As a proof of concept, we tuned the kinetics of fibril formation by varying the pH and measured the subsequent exponential increase of fibril vol...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Optical Society of America
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3370983/ https://www.ncbi.nlm.nih.gov/pubmed/22741089 http://dx.doi.org/10.1364/BOE.3.001446 |