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Osteosarcoma Phenotype Is Inhibited by 3,4-Methylenedioxy-β-nitrostyrene

β-nitrostyrene compounds, such as 3,4-methylenedioxy-β-nitrostyrene (MNS), inhibit growth and induce apoptosis in tumor cells, but no reports have investigated their role in osteosarcoma. In this study, human osteosarcoma cell families with cell lines of varying tumorigenic and metastatic potential...

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Autores principales: Messerschmitt, Patrick J., Rettew, Ashley N., Schroeder, Nicholas O., Brookover, Robert E., Jakatdar, Avanti P., Getty, Patrick J., Greenfield, Edward M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3371351/
https://www.ncbi.nlm.nih.gov/pubmed/22701331
http://dx.doi.org/10.1155/2012/479712
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author Messerschmitt, Patrick J.
Rettew, Ashley N.
Schroeder, Nicholas O.
Brookover, Robert E.
Jakatdar, Avanti P.
Getty, Patrick J.
Greenfield, Edward M.
author_facet Messerschmitt, Patrick J.
Rettew, Ashley N.
Schroeder, Nicholas O.
Brookover, Robert E.
Jakatdar, Avanti P.
Getty, Patrick J.
Greenfield, Edward M.
author_sort Messerschmitt, Patrick J.
collection PubMed
description β-nitrostyrene compounds, such as 3,4-methylenedioxy-β-nitrostyrene (MNS), inhibit growth and induce apoptosis in tumor cells, but no reports have investigated their role in osteosarcoma. In this study, human osteosarcoma cell families with cell lines of varying tumorigenic and metastatic potential were utilized. Scrape motility assays, colony formation assays, and colony survival assays were performed with osteosarcoma cell lines, both in the presence and absence of MNS. Effects of MNS on human osteoblasts and airway epithelial cells were assessed in monolayer cultures. MNS decreased metastatic cell line motility by 72–76% and colony formation by 95–100%. MNS consistently disrupted preformed colonies in a time-dependent and dose-dependent manner. MNS had similar effects on human osteoblasts but little effect on airway epithelial cells. An inactive analog of MNS had no detectable effects, demonstrating specificity. MNS decreases motility and colony formation of osteosarcoma cells and disrupts preformed cell colonies, while producing little effect on pulmonary epithelial cells.
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spelling pubmed-33713512012-06-14 Osteosarcoma Phenotype Is Inhibited by 3,4-Methylenedioxy-β-nitrostyrene Messerschmitt, Patrick J. Rettew, Ashley N. Schroeder, Nicholas O. Brookover, Robert E. Jakatdar, Avanti P. Getty, Patrick J. Greenfield, Edward M. Sarcoma Research Article β-nitrostyrene compounds, such as 3,4-methylenedioxy-β-nitrostyrene (MNS), inhibit growth and induce apoptosis in tumor cells, but no reports have investigated their role in osteosarcoma. In this study, human osteosarcoma cell families with cell lines of varying tumorigenic and metastatic potential were utilized. Scrape motility assays, colony formation assays, and colony survival assays were performed with osteosarcoma cell lines, both in the presence and absence of MNS. Effects of MNS on human osteoblasts and airway epithelial cells were assessed in monolayer cultures. MNS decreased metastatic cell line motility by 72–76% and colony formation by 95–100%. MNS consistently disrupted preformed colonies in a time-dependent and dose-dependent manner. MNS had similar effects on human osteoblasts but little effect on airway epithelial cells. An inactive analog of MNS had no detectable effects, demonstrating specificity. MNS decreases motility and colony formation of osteosarcoma cells and disrupts preformed cell colonies, while producing little effect on pulmonary epithelial cells. Hindawi Publishing Corporation 2012 2012-05-31 /pmc/articles/PMC3371351/ /pubmed/22701331 http://dx.doi.org/10.1155/2012/479712 Text en Copyright © 2012 Patrick J. Messerschmitt et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Messerschmitt, Patrick J.
Rettew, Ashley N.
Schroeder, Nicholas O.
Brookover, Robert E.
Jakatdar, Avanti P.
Getty, Patrick J.
Greenfield, Edward M.
Osteosarcoma Phenotype Is Inhibited by 3,4-Methylenedioxy-β-nitrostyrene
title Osteosarcoma Phenotype Is Inhibited by 3,4-Methylenedioxy-β-nitrostyrene
title_full Osteosarcoma Phenotype Is Inhibited by 3,4-Methylenedioxy-β-nitrostyrene
title_fullStr Osteosarcoma Phenotype Is Inhibited by 3,4-Methylenedioxy-β-nitrostyrene
title_full_unstemmed Osteosarcoma Phenotype Is Inhibited by 3,4-Methylenedioxy-β-nitrostyrene
title_short Osteosarcoma Phenotype Is Inhibited by 3,4-Methylenedioxy-β-nitrostyrene
title_sort osteosarcoma phenotype is inhibited by 3,4-methylenedioxy-β-nitrostyrene
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3371351/
https://www.ncbi.nlm.nih.gov/pubmed/22701331
http://dx.doi.org/10.1155/2012/479712
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