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Ultrasound-assisted extraction of total flavonoids from Inula helenium
BACKGROUND: Inula helenium was a perennial herb belonging to composite family and the roots of I. helenium have been widely used in traditional Chinese medicine. In this study, I. helenium was used as an experimental matrix. MATERIALS AND METHODS: Ultrasound-assisted extraction (UAE) of total flavon...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3371440/ https://www.ncbi.nlm.nih.gov/pubmed/22701292 http://dx.doi.org/10.4103/0973-1296.96581 |
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author | Wang, Jin Zhao, Yong-Ming Guo, Chun-Yan Zhang, Shao-Meng Liu, Chun-Lei Zhang, Dan-Shen Bai, Xue-Mei |
author_facet | Wang, Jin Zhao, Yong-Ming Guo, Chun-Yan Zhang, Shao-Meng Liu, Chun-Lei Zhang, Dan-Shen Bai, Xue-Mei |
author_sort | Wang, Jin |
collection | PubMed |
description | BACKGROUND: Inula helenium was a perennial herb belonging to composite family and the roots of I. helenium have been widely used in traditional Chinese medicine. In this study, I. helenium was used as an experimental matrix. MATERIALS AND METHODS: Ultrasound-assisted extraction (UAE) of total flavonoids from I. helenium was studied with dual wavelength UV-VIS spectrophotometer. Effects of various factors including ratio of material to liquid, ultrasonic time, ethanol concentration and extraction times on extraction yield of total flavonoids were evaluated. Then, orthogonal design of four factors at three levels was applied for optimization the extraction yields of flavonoids from the root of I. helenium. RESULT: The optimal extracting process of the total flavonoids from the root of the I. helenium was 1 g plant sample with 20 ml of 60% ethanol, extracting twice and each time for 20 min. CONCLUSION: Under these optimal conditions, the yield of total flavonoids was (17.36±0.94) mg/g. UAE was more efficient and time saving for the extraction of flavonoids from plant materials. |
format | Online Article Text |
id | pubmed-3371440 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-33714402012-06-14 Ultrasound-assisted extraction of total flavonoids from Inula helenium Wang, Jin Zhao, Yong-Ming Guo, Chun-Yan Zhang, Shao-Meng Liu, Chun-Lei Zhang, Dan-Shen Bai, Xue-Mei Pharmacogn Mag Original Article BACKGROUND: Inula helenium was a perennial herb belonging to composite family and the roots of I. helenium have been widely used in traditional Chinese medicine. In this study, I. helenium was used as an experimental matrix. MATERIALS AND METHODS: Ultrasound-assisted extraction (UAE) of total flavonoids from I. helenium was studied with dual wavelength UV-VIS spectrophotometer. Effects of various factors including ratio of material to liquid, ultrasonic time, ethanol concentration and extraction times on extraction yield of total flavonoids were evaluated. Then, orthogonal design of four factors at three levels was applied for optimization the extraction yields of flavonoids from the root of I. helenium. RESULT: The optimal extracting process of the total flavonoids from the root of the I. helenium was 1 g plant sample with 20 ml of 60% ethanol, extracting twice and each time for 20 min. CONCLUSION: Under these optimal conditions, the yield of total flavonoids was (17.36±0.94) mg/g. UAE was more efficient and time saving for the extraction of flavonoids from plant materials. Medknow Publications & Media Pvt Ltd 2012 /pmc/articles/PMC3371440/ /pubmed/22701292 http://dx.doi.org/10.4103/0973-1296.96581 Text en Copyright: © Pharmacognosy Magazine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Wang, Jin Zhao, Yong-Ming Guo, Chun-Yan Zhang, Shao-Meng Liu, Chun-Lei Zhang, Dan-Shen Bai, Xue-Mei Ultrasound-assisted extraction of total flavonoids from Inula helenium |
title | Ultrasound-assisted extraction of total flavonoids from Inula helenium |
title_full | Ultrasound-assisted extraction of total flavonoids from Inula helenium |
title_fullStr | Ultrasound-assisted extraction of total flavonoids from Inula helenium |
title_full_unstemmed | Ultrasound-assisted extraction of total flavonoids from Inula helenium |
title_short | Ultrasound-assisted extraction of total flavonoids from Inula helenium |
title_sort | ultrasound-assisted extraction of total flavonoids from inula helenium |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3371440/ https://www.ncbi.nlm.nih.gov/pubmed/22701292 http://dx.doi.org/10.4103/0973-1296.96581 |
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