Ultra-low-input, tagmentation-based whole-genome bisulfite sequencing

We have adapted transposase-based in vitro shotgun library construction (“tagmentation”) for whole-genome bisulfite sequencing. This method, Tn5mC-seq, enables a >100-fold reduction in starting material relative to conventional protocols, such that we generate highly complex bisulfite sequencing...

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Detalles Bibliográficos
Autores principales: Adey, Andrew, Shendure, Jay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2012
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3371708/
https://www.ncbi.nlm.nih.gov/pubmed/22466172
http://dx.doi.org/10.1101/gr.136242.111

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3371708/
https://www.ncbi.nlm.nih.gov/pubmed/22466172
http://dx.doi.org/10.1101/gr.136242.111

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