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Fluoxetine Increases the Expression of NCAM140 and pCREB in Rat C6 Glioma Cells

OBJECTIVE: Dysfunction of neural plasticity in the brain is known to alter neural networks, resulting in depression. To understand how fluoxetine regulates molecules involved in neural plasticity, the expression levels of NCAM, NCAM140, CREB and pCREB, in rat C6 glioma cells after fluoxetine treatme...

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Autores principales: Choi, Mi Ran, Oh, Dong Hoon, Kim, Seok Hyeon, Jung, Kyoung Hwa, Das, Nando Dulal, Chai, Young Gyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Neuropsychiatric Association 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3372567/
https://www.ncbi.nlm.nih.gov/pubmed/22707970
http://dx.doi.org/10.4306/pi.2012.9.2.180
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author Choi, Mi Ran
Oh, Dong Hoon
Kim, Seok Hyeon
Jung, Kyoung Hwa
Das, Nando Dulal
Chai, Young Gyu
author_facet Choi, Mi Ran
Oh, Dong Hoon
Kim, Seok Hyeon
Jung, Kyoung Hwa
Das, Nando Dulal
Chai, Young Gyu
author_sort Choi, Mi Ran
collection PubMed
description OBJECTIVE: Dysfunction of neural plasticity in the brain is known to alter neural networks, resulting in depression. To understand how fluoxetine regulates molecules involved in neural plasticity, the expression levels of NCAM, NCAM140, CREB and pCREB, in rat C6 glioma cells after fluoxetine treatment were examined. METHODS: C6 cells were cultured after 20 min or after 6, 24 or 72 h treatments with 10 µM fluoxetine. Immunocytochemistry was used to determine the effect of fluoxetine on the expression of NCAM. Western blot analysis was used to measure the expression levels of NCAM140 and CREB and the induction of pCREB after fluoxetine treatment. RESULTS: NCAM expression following 72-h fluoxetine treatment was significantly increased around cell membranes compared to control cells. Cells treated with fluoxetine for 6 and 72 h showed a significant increase in NCAM140 expression compared to cells treated for 20 min. The level of pCREB in the cells treated with fluoxetine for 72 h not only increased more than 60%, but was also significantly different when compared with the other treatment times. The 72-h fluoxetine treatment led to the increase of NCAM140 and the phosphorylation of CREB in C6 cells. CONCLUSION: Our findings indicate that fluoxetine treatment regulates neuronal plasticity and neurite outgrowth by phosphorylating and activating CREB via the NCAM140 homophilic interaction-induced activation of the Ras-MAPK pathway.
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spelling pubmed-33725672012-06-15 Fluoxetine Increases the Expression of NCAM140 and pCREB in Rat C6 Glioma Cells Choi, Mi Ran Oh, Dong Hoon Kim, Seok Hyeon Jung, Kyoung Hwa Das, Nando Dulal Chai, Young Gyu Psychiatry Investig Original Article OBJECTIVE: Dysfunction of neural plasticity in the brain is known to alter neural networks, resulting in depression. To understand how fluoxetine regulates molecules involved in neural plasticity, the expression levels of NCAM, NCAM140, CREB and pCREB, in rat C6 glioma cells after fluoxetine treatment were examined. METHODS: C6 cells were cultured after 20 min or after 6, 24 or 72 h treatments with 10 µM fluoxetine. Immunocytochemistry was used to determine the effect of fluoxetine on the expression of NCAM. Western blot analysis was used to measure the expression levels of NCAM140 and CREB and the induction of pCREB after fluoxetine treatment. RESULTS: NCAM expression following 72-h fluoxetine treatment was significantly increased around cell membranes compared to control cells. Cells treated with fluoxetine for 6 and 72 h showed a significant increase in NCAM140 expression compared to cells treated for 20 min. The level of pCREB in the cells treated with fluoxetine for 72 h not only increased more than 60%, but was also significantly different when compared with the other treatment times. The 72-h fluoxetine treatment led to the increase of NCAM140 and the phosphorylation of CREB in C6 cells. CONCLUSION: Our findings indicate that fluoxetine treatment regulates neuronal plasticity and neurite outgrowth by phosphorylating and activating CREB via the NCAM140 homophilic interaction-induced activation of the Ras-MAPK pathway. Korean Neuropsychiatric Association 2012-06 2012-01-09 /pmc/articles/PMC3372567/ /pubmed/22707970 http://dx.doi.org/10.4306/pi.2012.9.2.180 Text en Copyright © 2012 Korean Neuropsychiatric Association http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Choi, Mi Ran
Oh, Dong Hoon
Kim, Seok Hyeon
Jung, Kyoung Hwa
Das, Nando Dulal
Chai, Young Gyu
Fluoxetine Increases the Expression of NCAM140 and pCREB in Rat C6 Glioma Cells
title Fluoxetine Increases the Expression of NCAM140 and pCREB in Rat C6 Glioma Cells
title_full Fluoxetine Increases the Expression of NCAM140 and pCREB in Rat C6 Glioma Cells
title_fullStr Fluoxetine Increases the Expression of NCAM140 and pCREB in Rat C6 Glioma Cells
title_full_unstemmed Fluoxetine Increases the Expression of NCAM140 and pCREB in Rat C6 Glioma Cells
title_short Fluoxetine Increases the Expression of NCAM140 and pCREB in Rat C6 Glioma Cells
title_sort fluoxetine increases the expression of ncam140 and pcreb in rat c6 glioma cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3372567/
https://www.ncbi.nlm.nih.gov/pubmed/22707970
http://dx.doi.org/10.4306/pi.2012.9.2.180
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