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Cytotoxic and Immunochemical Properties of Viscumin Encapsulated 
in Polylactide Microparticles

Biodegradable polylactide microparticles with encapsulated cytotoxic protein viscumin were obtained via the ultrasound-assisted supercritical fluid technique. The size of the microparticles was 10–50 µM, as shown by electron microscopy. The time course of viscumin release from microparticles was stu...

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Autores principales: Kolotova, E.S., Egorova, S.G., Ramonova, A.A., Bogorodski, S.E., Popov, V.K., Agapov, 
I.I., Kirpichnikov, M.P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A.I. Gordeyev 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3372992/
https://www.ncbi.nlm.nih.gov/pubmed/22708070
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author Kolotova, E.S.
Egorova, S.G.
Ramonova, A.A.
Bogorodski, S.E.
Popov, V.K.
Agapov, 
I.I.
Kirpichnikov, M.P.
author_facet Kolotova, E.S.
Egorova, S.G.
Ramonova, A.A.
Bogorodski, S.E.
Popov, V.K.
Agapov, 
I.I.
Kirpichnikov, M.P.
author_sort Kolotova, E.S.
collection PubMed
description Biodegradable polylactide microparticles with encapsulated cytotoxic protein viscumin were obtained via the ultrasound-assisted supercritical fluid technique. The size of the microparticles was 10–50 µM, as shown by electron microscopy. The time course of viscumin release from microparticles was studied using an immunoenzyme test system with anti-viscumin monoclonal antibodies. It was found that 99.91% of the cytotoxic protein was incorporated into polymer microparticles. Only 0.08% of the initially encapsulated viscumin was released from the microparticles following incubation for 120 h in a phosphate-buffered saline at neutral pH. Importantly, the method of ultrasonic dry supercritical fluid encapsulation failed to alter both the cytotoxic potency and the immunochemical properties of the encapsulated viscumin. Thus, this procedure can be used to generate biodegradable polylactide microparticles with encapsulated bioactive substances.
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spelling pubmed-33729922012-06-15 Cytotoxic and Immunochemical Properties of Viscumin Encapsulated 
in Polylactide Microparticles Kolotova, E.S. Egorova, S.G. Ramonova, A.A. Bogorodski, S.E. Popov, V.K. Agapov, 
I.I. Kirpichnikov, M.P. Acta Naturae Research Article Biodegradable polylactide microparticles with encapsulated cytotoxic protein viscumin were obtained via the ultrasound-assisted supercritical fluid technique. The size of the microparticles was 10–50 µM, as shown by electron microscopy. The time course of viscumin release from microparticles was studied using an immunoenzyme test system with anti-viscumin monoclonal antibodies. It was found that 99.91% of the cytotoxic protein was incorporated into polymer microparticles. Only 0.08% of the initially encapsulated viscumin was released from the microparticles following incubation for 120 h in a phosphate-buffered saline at neutral pH. Importantly, the method of ultrasonic dry supercritical fluid encapsulation failed to alter both the cytotoxic potency and the immunochemical properties of the encapsulated viscumin. Thus, this procedure can be used to generate biodegradable polylactide microparticles with encapsulated bioactive substances. A.I. Gordeyev 2012 /pmc/articles/PMC3372992/ /pubmed/22708070 Text en Copyright © 2012 Park-media Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kolotova, E.S.
Egorova, S.G.
Ramonova, A.A.
Bogorodski, S.E.
Popov, V.K.
Agapov, 
I.I.
Kirpichnikov, M.P.
Cytotoxic and Immunochemical Properties of Viscumin Encapsulated 
in Polylactide Microparticles
title Cytotoxic and Immunochemical Properties of Viscumin Encapsulated 
in Polylactide Microparticles
title_full Cytotoxic and Immunochemical Properties of Viscumin Encapsulated 
in Polylactide Microparticles
title_fullStr Cytotoxic and Immunochemical Properties of Viscumin Encapsulated 
in Polylactide Microparticles
title_full_unstemmed Cytotoxic and Immunochemical Properties of Viscumin Encapsulated 
in Polylactide Microparticles
title_short Cytotoxic and Immunochemical Properties of Viscumin Encapsulated 
in Polylactide Microparticles
title_sort cytotoxic and immunochemical properties of viscumin encapsulated 
in polylactide microparticles
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3372992/
https://www.ncbi.nlm.nih.gov/pubmed/22708070
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