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Genetic Divergence of Toxoplasma gondii Strains Associated with Ocular Toxoplasmosis, Brazil

Previous studies have shown a high prevalence of toxoplasmosis and the frequent occurrence of ocular disease in Brazil. To identify the genotypes of parasite strains associated with ocular disease, we compared 25 clinical and animal isolates of Toxoplasma gondii from Brazil to previously characteriz...

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Autores principales: Khan, Asis, Jordan, Catherine, Muccioli, Cristina, Vallochi, Adriana L., Rizzo, Luiz V., Belfort, Rubens, Vitor, Ricardo W.A., Silveira, Claudio, Sibley, L. David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3373049/
https://www.ncbi.nlm.nih.gov/pubmed/16707050
http://dx.doi.org/10.3201/eid1206.060025
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author Khan, Asis
Jordan, Catherine
Muccioli, Cristina
Vallochi, Adriana L.
Rizzo, Luiz V.
Belfort, Rubens
Vitor, Ricardo W.A.
Silveira, Claudio
Sibley, L. David
author_facet Khan, Asis
Jordan, Catherine
Muccioli, Cristina
Vallochi, Adriana L.
Rizzo, Luiz V.
Belfort, Rubens
Vitor, Ricardo W.A.
Silveira, Claudio
Sibley, L. David
author_sort Khan, Asis
collection PubMed
description Previous studies have shown a high prevalence of toxoplasmosis and the frequent occurrence of ocular disease in Brazil. To identify the genotypes of parasite strains associated with ocular disease, we compared 25 clinical and animal isolates of Toxoplasma gondii from Brazil to previously characterized clonal lineages from North America and Europe. Multilocus nested polymerase chain reaction analysis was combined with direct sequencing of a polymorphic intron to classify strains by phylogenetic methods. The genotypes of T. gondii strains isolated from Brazil were highly divergent when compared to the previously described clonal lineages. Several new predominant genotypes were identified from different regions of Brazil, including 2 small outbreaks attributable to foodborne or waterborne infection. These findings show that the genetic makeup of T. gondii is more complex than previously recognized and suggest that unique or divergent genotypes may contribute to different clinical outcomes of toxoplasmosis in different localities.
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spelling pubmed-33730492012-06-13 Genetic Divergence of Toxoplasma gondii Strains Associated with Ocular Toxoplasmosis, Brazil Khan, Asis Jordan, Catherine Muccioli, Cristina Vallochi, Adriana L. Rizzo, Luiz V. Belfort, Rubens Vitor, Ricardo W.A. Silveira, Claudio Sibley, L. David Emerg Infect Dis Research Previous studies have shown a high prevalence of toxoplasmosis and the frequent occurrence of ocular disease in Brazil. To identify the genotypes of parasite strains associated with ocular disease, we compared 25 clinical and animal isolates of Toxoplasma gondii from Brazil to previously characterized clonal lineages from North America and Europe. Multilocus nested polymerase chain reaction analysis was combined with direct sequencing of a polymorphic intron to classify strains by phylogenetic methods. The genotypes of T. gondii strains isolated from Brazil were highly divergent when compared to the previously described clonal lineages. Several new predominant genotypes were identified from different regions of Brazil, including 2 small outbreaks attributable to foodborne or waterborne infection. These findings show that the genetic makeup of T. gondii is more complex than previously recognized and suggest that unique or divergent genotypes may contribute to different clinical outcomes of toxoplasmosis in different localities. Centers for Disease Control and Prevention 2006-06 /pmc/articles/PMC3373049/ /pubmed/16707050 http://dx.doi.org/10.3201/eid1206.060025 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research
Khan, Asis
Jordan, Catherine
Muccioli, Cristina
Vallochi, Adriana L.
Rizzo, Luiz V.
Belfort, Rubens
Vitor, Ricardo W.A.
Silveira, Claudio
Sibley, L. David
Genetic Divergence of Toxoplasma gondii Strains Associated with Ocular Toxoplasmosis, Brazil
title Genetic Divergence of Toxoplasma gondii Strains Associated with Ocular Toxoplasmosis, Brazil
title_full Genetic Divergence of Toxoplasma gondii Strains Associated with Ocular Toxoplasmosis, Brazil
title_fullStr Genetic Divergence of Toxoplasma gondii Strains Associated with Ocular Toxoplasmosis, Brazil
title_full_unstemmed Genetic Divergence of Toxoplasma gondii Strains Associated with Ocular Toxoplasmosis, Brazil
title_short Genetic Divergence of Toxoplasma gondii Strains Associated with Ocular Toxoplasmosis, Brazil
title_sort genetic divergence of toxoplasma gondii strains associated with ocular toxoplasmosis, brazil
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3373049/
https://www.ncbi.nlm.nih.gov/pubmed/16707050
http://dx.doi.org/10.3201/eid1206.060025
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