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Genetic Divergence of Toxoplasma gondii Strains Associated with Ocular Toxoplasmosis, Brazil
Previous studies have shown a high prevalence of toxoplasmosis and the frequent occurrence of ocular disease in Brazil. To identify the genotypes of parasite strains associated with ocular disease, we compared 25 clinical and animal isolates of Toxoplasma gondii from Brazil to previously characteriz...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Centers for Disease Control and Prevention
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3373049/ https://www.ncbi.nlm.nih.gov/pubmed/16707050 http://dx.doi.org/10.3201/eid1206.060025 |
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author | Khan, Asis Jordan, Catherine Muccioli, Cristina Vallochi, Adriana L. Rizzo, Luiz V. Belfort, Rubens Vitor, Ricardo W.A. Silveira, Claudio Sibley, L. David |
author_facet | Khan, Asis Jordan, Catherine Muccioli, Cristina Vallochi, Adriana L. Rizzo, Luiz V. Belfort, Rubens Vitor, Ricardo W.A. Silveira, Claudio Sibley, L. David |
author_sort | Khan, Asis |
collection | PubMed |
description | Previous studies have shown a high prevalence of toxoplasmosis and the frequent occurrence of ocular disease in Brazil. To identify the genotypes of parasite strains associated with ocular disease, we compared 25 clinical and animal isolates of Toxoplasma gondii from Brazil to previously characterized clonal lineages from North America and Europe. Multilocus nested polymerase chain reaction analysis was combined with direct sequencing of a polymorphic intron to classify strains by phylogenetic methods. The genotypes of T. gondii strains isolated from Brazil were highly divergent when compared to the previously described clonal lineages. Several new predominant genotypes were identified from different regions of Brazil, including 2 small outbreaks attributable to foodborne or waterborne infection. These findings show that the genetic makeup of T. gondii is more complex than previously recognized and suggest that unique or divergent genotypes may contribute to different clinical outcomes of toxoplasmosis in different localities. |
format | Online Article Text |
id | pubmed-3373049 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | Centers for Disease Control and Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-33730492012-06-13 Genetic Divergence of Toxoplasma gondii Strains Associated with Ocular Toxoplasmosis, Brazil Khan, Asis Jordan, Catherine Muccioli, Cristina Vallochi, Adriana L. Rizzo, Luiz V. Belfort, Rubens Vitor, Ricardo W.A. Silveira, Claudio Sibley, L. David Emerg Infect Dis Research Previous studies have shown a high prevalence of toxoplasmosis and the frequent occurrence of ocular disease in Brazil. To identify the genotypes of parasite strains associated with ocular disease, we compared 25 clinical and animal isolates of Toxoplasma gondii from Brazil to previously characterized clonal lineages from North America and Europe. Multilocus nested polymerase chain reaction analysis was combined with direct sequencing of a polymorphic intron to classify strains by phylogenetic methods. The genotypes of T. gondii strains isolated from Brazil were highly divergent when compared to the previously described clonal lineages. Several new predominant genotypes were identified from different regions of Brazil, including 2 small outbreaks attributable to foodborne or waterborne infection. These findings show that the genetic makeup of T. gondii is more complex than previously recognized and suggest that unique or divergent genotypes may contribute to different clinical outcomes of toxoplasmosis in different localities. Centers for Disease Control and Prevention 2006-06 /pmc/articles/PMC3373049/ /pubmed/16707050 http://dx.doi.org/10.3201/eid1206.060025 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited. |
spellingShingle | Research Khan, Asis Jordan, Catherine Muccioli, Cristina Vallochi, Adriana L. Rizzo, Luiz V. Belfort, Rubens Vitor, Ricardo W.A. Silveira, Claudio Sibley, L. David Genetic Divergence of Toxoplasma gondii Strains Associated with Ocular Toxoplasmosis, Brazil |
title | Genetic Divergence of Toxoplasma gondii Strains Associated with Ocular Toxoplasmosis, Brazil |
title_full | Genetic Divergence of Toxoplasma gondii Strains Associated with Ocular Toxoplasmosis, Brazil |
title_fullStr | Genetic Divergence of Toxoplasma gondii Strains Associated with Ocular Toxoplasmosis, Brazil |
title_full_unstemmed | Genetic Divergence of Toxoplasma gondii Strains Associated with Ocular Toxoplasmosis, Brazil |
title_short | Genetic Divergence of Toxoplasma gondii Strains Associated with Ocular Toxoplasmosis, Brazil |
title_sort | genetic divergence of toxoplasma gondii strains associated with ocular toxoplasmosis, brazil |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3373049/ https://www.ncbi.nlm.nih.gov/pubmed/16707050 http://dx.doi.org/10.3201/eid1206.060025 |
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