Helicobacter pullorum in Chickens, Belgium

A total of 110 broilers from 11 flocks were tested for Helicobacter pullorum by polymerase chain reaction; positive samples were reexamined with a conventional isolation method. H. pullorum isolates were examined by amplified fragment length polymorphism (AFLP) fingerprinting for interstrain genetic...

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Autores principales: Ceelen, Liesbeth M., Decostere, Annemie, Van den Bulck, Kathleen, On, Stephen L.W., Baele, Margo, Ducatelle, Richard, Haesebrouck, Freddy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2006
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3373087/
https://www.ncbi.nlm.nih.gov/pubmed/16494752
http://dx.doi.org/10.3201/eid1202.050847
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author Ceelen, Liesbeth M.
Decostere, Annemie
Van den Bulck, Kathleen
On, Stephen L.W.
Baele, Margo
Ducatelle, Richard
Haesebrouck, Freddy
author_facet Ceelen, Liesbeth M.
Decostere, Annemie
Van den Bulck, Kathleen
On, Stephen L.W.
Baele, Margo
Ducatelle, Richard
Haesebrouck, Freddy
author_sort Ceelen, Liesbeth M.
collection PubMed
description A total of 110 broilers from 11 flocks were tested for Helicobacter pullorum by polymerase chain reaction; positive samples were reexamined with a conventional isolation method. H. pullorum isolates were examined by amplified fragment length polymorphism (AFLP) fingerprinting for interstrain genetic diversity and relatedness. Sixteen isolates from cecal samples from 2 different flocks were obtained. AFLP analysis showed that these isolates and 4 additional isolates from a different flock clustered according to their origin, which indicates that H. pullorum colonization may occur with a single strain that disseminates throughout the flock. Strains isolated from different hosts or geographic sources displayed a distinctive pattern. H. pullorum is present in approximately one third of live chickens in Belgium and may represent a risk to human health.
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spelling pubmed-33730872012-06-13 Helicobacter pullorum in Chickens, Belgium Ceelen, Liesbeth M. Decostere, Annemie Van den Bulck, Kathleen On, Stephen L.W. Baele, Margo Ducatelle, Richard Haesebrouck, Freddy Emerg Infect Dis Research A total of 110 broilers from 11 flocks were tested for Helicobacter pullorum by polymerase chain reaction; positive samples were reexamined with a conventional isolation method. H. pullorum isolates were examined by amplified fragment length polymorphism (AFLP) fingerprinting for interstrain genetic diversity and relatedness. Sixteen isolates from cecal samples from 2 different flocks were obtained. AFLP analysis showed that these isolates and 4 additional isolates from a different flock clustered according to their origin, which indicates that H. pullorum colonization may occur with a single strain that disseminates throughout the flock. Strains isolated from different hosts or geographic sources displayed a distinctive pattern. H. pullorum is present in approximately one third of live chickens in Belgium and may represent a risk to human health. Centers for Disease Control and Prevention 2006-02 /pmc/articles/PMC3373087/ /pubmed/16494752 http://dx.doi.org/10.3201/eid1202.050847 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research
Ceelen, Liesbeth M.
Decostere, Annemie
Van den Bulck, Kathleen
On, Stephen L.W.
Baele, Margo
Ducatelle, Richard
Haesebrouck, Freddy
Helicobacter pullorum in Chickens, Belgium
title Helicobacter pullorum in Chickens, Belgium
title_full Helicobacter pullorum in Chickens, Belgium
title_fullStr Helicobacter pullorum in Chickens, Belgium
title_full_unstemmed Helicobacter pullorum in Chickens, Belgium
title_short Helicobacter pullorum in Chickens, Belgium
title_sort helicobacter pullorum in chickens, belgium
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3373087/
https://www.ncbi.nlm.nih.gov/pubmed/16494752
http://dx.doi.org/10.3201/eid1202.050847
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AT baelemargo helicobacterpulloruminchickensbelgium
AT ducatellerichard helicobacterpulloruminchickensbelgium
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