Determination of the Proportion of Total Soil Extracellular Acid Phosphomonoesterase (E.C. 3.1.3.2) Activity Represented by Roots in the Soil of Different Forest Ecosystems

The aim of this study is to present a new method for determining the root-derived extracellular acid phosphomonoesterase (EAPM) activity fraction within the total EAPM activity of soil. EAPM activity was determined for roots, organic and mineral soil. Samples were collected using paired PVC cylinder...

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Detalles Bibliográficos
Autores principales: Rejsek, Klement, Vranova, Valerie, Formanek, Pavel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific World Journal 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3373123/
https://www.ncbi.nlm.nih.gov/pubmed/22701351
http://dx.doi.org/10.1100/2012/250805
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author Rejsek, Klement
Vranova, Valerie
Formanek, Pavel
author_facet Rejsek, Klement
Vranova, Valerie
Formanek, Pavel
author_sort Rejsek, Klement
collection PubMed
description The aim of this study is to present a new method for determining the root-derived extracellular acid phosphomonoesterase (EAPM) activity fraction within the total EAPM activity of soil. EAPM activity was determined for roots, organic and mineral soil. Samples were collected using paired PVC cylinders, inserted to a depth of 15 cm, within seven selected forest stands. Root-derived EAPM formed between 4 and18% of the total EAPM activity of soil from forests of differing maturity. A new approach, presented in this work, enables separation of root-derived EAPM activity from total soil EAPM. Separation of root-derived EAPM from soil provides a better understanding of its role in P-cycling in terrestrial ecosystems. The method presented in this work is a first step towards the separation of root- and microbe-derived EAPM in soils, which are thought to possess different kinetic properties and different sensitivity to environmental change.
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spelling pubmed-33731232012-06-14 Determination of the Proportion of Total Soil Extracellular Acid Phosphomonoesterase (E.C. 3.1.3.2) Activity Represented by Roots in the Soil of Different Forest Ecosystems Rejsek, Klement Vranova, Valerie Formanek, Pavel ScientificWorldJournal Research Article The aim of this study is to present a new method for determining the root-derived extracellular acid phosphomonoesterase (EAPM) activity fraction within the total EAPM activity of soil. EAPM activity was determined for roots, organic and mineral soil. Samples were collected using paired PVC cylinders, inserted to a depth of 15 cm, within seven selected forest stands. Root-derived EAPM formed between 4 and18% of the total EAPM activity of soil from forests of differing maturity. A new approach, presented in this work, enables separation of root-derived EAPM activity from total soil EAPM. Separation of root-derived EAPM from soil provides a better understanding of its role in P-cycling in terrestrial ecosystems. The method presented in this work is a first step towards the separation of root- and microbe-derived EAPM in soils, which are thought to possess different kinetic properties and different sensitivity to environmental change. The Scientific World Journal 2012-06-04 /pmc/articles/PMC3373123/ /pubmed/22701351 http://dx.doi.org/10.1100/2012/250805 Text en Copyright © 2012 Klement Rejsek et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rejsek, Klement
Vranova, Valerie
Formanek, Pavel
Determination of the Proportion of Total Soil Extracellular Acid Phosphomonoesterase (E.C. 3.1.3.2) Activity Represented by Roots in the Soil of Different Forest Ecosystems
title Determination of the Proportion of Total Soil Extracellular Acid Phosphomonoesterase (E.C. 3.1.3.2) Activity Represented by Roots in the Soil of Different Forest Ecosystems
title_full Determination of the Proportion of Total Soil Extracellular Acid Phosphomonoesterase (E.C. 3.1.3.2) Activity Represented by Roots in the Soil of Different Forest Ecosystems
title_fullStr Determination of the Proportion of Total Soil Extracellular Acid Phosphomonoesterase (E.C. 3.1.3.2) Activity Represented by Roots in the Soil of Different Forest Ecosystems
title_full_unstemmed Determination of the Proportion of Total Soil Extracellular Acid Phosphomonoesterase (E.C. 3.1.3.2) Activity Represented by Roots in the Soil of Different Forest Ecosystems
title_short Determination of the Proportion of Total Soil Extracellular Acid Phosphomonoesterase (E.C. 3.1.3.2) Activity Represented by Roots in the Soil of Different Forest Ecosystems
title_sort determination of the proportion of total soil extracellular acid phosphomonoesterase (e.c. 3.1.3.2) activity represented by roots in the soil of different forest ecosystems
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3373123/
https://www.ncbi.nlm.nih.gov/pubmed/22701351
http://dx.doi.org/10.1100/2012/250805
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AT formanekpavel determinationoftheproportionoftotalsoilextracellularacidphosphomonoesteraseec3132activityrepresentedbyrootsinthesoilofdifferentforestecosystems

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