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Macrophages participate in host protection and the disease pathology associated with Leishmania braziliensis infection

BACKGROUND: Leishmania preferentially infects macrophages, which allow the parasite to multiply but can also kill the parasite. Although the T cell response in human leishmaniasis is well-characterized, little is known about the concomitant macrophage behavior. The aim of this study was to character...

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Autores principales: Giudice, Angela, Vendrame, Célia, Bezerra, Caroline, Carvalho, Lucas P, Delavechia, Thaís, Carvalho, Edgar M, Bacellar, Olívia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3373377/
https://www.ncbi.nlm.nih.gov/pubmed/22458474
http://dx.doi.org/10.1186/1471-2334-12-75
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author Giudice, Angela
Vendrame, Célia
Bezerra, Caroline
Carvalho, Lucas P
Delavechia, Thaís
Carvalho, Edgar M
Bacellar, Olívia
author_facet Giudice, Angela
Vendrame, Célia
Bezerra, Caroline
Carvalho, Lucas P
Delavechia, Thaís
Carvalho, Edgar M
Bacellar, Olívia
author_sort Giudice, Angela
collection PubMed
description BACKGROUND: Leishmania preferentially infects macrophages, which allow the parasite to multiply but can also kill the parasite. Although the T cell response in human leishmaniasis is well-characterized, little is known about the concomitant macrophage behavior. The aim of this study was to characterize the macrophage immune response after Leishmania braziliensis infection in cells derived from cutaneous leishmaniasis (CL) or mucosal leishmaniasis (ML) patients, subclinical individuals (SC) and healthy control subjects (HS). METHODS: Peripheral blood mononuclear cell-derived macrophages from the different groups were exposed to L. braziliensis in vitro and were evaluated for susceptibility to Leishmania infection, ability to kill Leishmania and chemokine/cytokine production. Nitric Oxide (NO) and superoxide (O(2)(-)) levels in the supernatant of infected macrophage cultures were monitored. RESULTS: After exposure to L. braziliensis, peripheral blood mononuclear cell-derived macrophages from SC individuals showed a lower infection rate and a smaller number of intracellular amastigotes compared to cells from CL and ML patients. Macrophages from CL and ML patients produced more chemokines and TNF-α than those from the SC group. Production of NO and O(2)(- )were detected but did not vary significantly among the different groups. CONCLUSIONS: Our data indicate that macrophages play a pivotal role in controlling L. braziliensis infection and in leishmaniasis pathology by secreting pro-inflammatory chemokines/cytokines that activate and recruit T cells, overwhelming the inflammatory response.
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spelling pubmed-33733772012-06-13 Macrophages participate in host protection and the disease pathology associated with Leishmania braziliensis infection Giudice, Angela Vendrame, Célia Bezerra, Caroline Carvalho, Lucas P Delavechia, Thaís Carvalho, Edgar M Bacellar, Olívia BMC Infect Dis Research Article BACKGROUND: Leishmania preferentially infects macrophages, which allow the parasite to multiply but can also kill the parasite. Although the T cell response in human leishmaniasis is well-characterized, little is known about the concomitant macrophage behavior. The aim of this study was to characterize the macrophage immune response after Leishmania braziliensis infection in cells derived from cutaneous leishmaniasis (CL) or mucosal leishmaniasis (ML) patients, subclinical individuals (SC) and healthy control subjects (HS). METHODS: Peripheral blood mononuclear cell-derived macrophages from the different groups were exposed to L. braziliensis in vitro and were evaluated for susceptibility to Leishmania infection, ability to kill Leishmania and chemokine/cytokine production. Nitric Oxide (NO) and superoxide (O(2)(-)) levels in the supernatant of infected macrophage cultures were monitored. RESULTS: After exposure to L. braziliensis, peripheral blood mononuclear cell-derived macrophages from SC individuals showed a lower infection rate and a smaller number of intracellular amastigotes compared to cells from CL and ML patients. Macrophages from CL and ML patients produced more chemokines and TNF-α than those from the SC group. Production of NO and O(2)(- )were detected but did not vary significantly among the different groups. CONCLUSIONS: Our data indicate that macrophages play a pivotal role in controlling L. braziliensis infection and in leishmaniasis pathology by secreting pro-inflammatory chemokines/cytokines that activate and recruit T cells, overwhelming the inflammatory response. BioMed Central 2012-03-29 /pmc/articles/PMC3373377/ /pubmed/22458474 http://dx.doi.org/10.1186/1471-2334-12-75 Text en Copyright ©2012 Giudice et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Giudice, Angela
Vendrame, Célia
Bezerra, Caroline
Carvalho, Lucas P
Delavechia, Thaís
Carvalho, Edgar M
Bacellar, Olívia
Macrophages participate in host protection and the disease pathology associated with Leishmania braziliensis infection
title Macrophages participate in host protection and the disease pathology associated with Leishmania braziliensis infection
title_full Macrophages participate in host protection and the disease pathology associated with Leishmania braziliensis infection
title_fullStr Macrophages participate in host protection and the disease pathology associated with Leishmania braziliensis infection
title_full_unstemmed Macrophages participate in host protection and the disease pathology associated with Leishmania braziliensis infection
title_short Macrophages participate in host protection and the disease pathology associated with Leishmania braziliensis infection
title_sort macrophages participate in host protection and the disease pathology associated with leishmania braziliensis infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3373377/
https://www.ncbi.nlm.nih.gov/pubmed/22458474
http://dx.doi.org/10.1186/1471-2334-12-75
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