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2D protrusion but not motility predicts growth factor–induced cancer cell migration in 3D collagen

Growth factor–induced migration is a critical step in the dissemination and metastasis of solid tumors. Although differences in properties characterizing cell migration on two-dimensional (2D) substrata versus within three-dimensional (3D) matrices have been noted for particular growth factor stimul...

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Detalles Bibliográficos
Autores principales: Meyer, Aaron S., Hughes-Alford, Shannon K., Kay, Jennifer E., Castillo, Amalchi, Wells, Alan, Gertler, Frank B., Lauffenburger, Douglas A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3373410/
https://www.ncbi.nlm.nih.gov/pubmed/22665521
http://dx.doi.org/10.1083/jcb.201201003
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author Meyer, Aaron S.
Hughes-Alford, Shannon K.
Kay, Jennifer E.
Castillo, Amalchi
Wells, Alan
Gertler, Frank B.
Lauffenburger, Douglas A.
author_facet Meyer, Aaron S.
Hughes-Alford, Shannon K.
Kay, Jennifer E.
Castillo, Amalchi
Wells, Alan
Gertler, Frank B.
Lauffenburger, Douglas A.
author_sort Meyer, Aaron S.
collection PubMed
description Growth factor–induced migration is a critical step in the dissemination and metastasis of solid tumors. Although differences in properties characterizing cell migration on two-dimensional (2D) substrata versus within three-dimensional (3D) matrices have been noted for particular growth factor stimuli, the 2D approach remains in more common use as an efficient surrogate, especially for high-throughput experiments. We therefore were motivated to investigate which migration properties measured in various 2D assays might be reflective of 3D migratory behavioral responses. We used human triple-negative breast cancer lines stimulated by a panel of receptor tyrosine kinase ligands relevant to mammary carcinoma progression. Whereas 2D migration properties did not correlate well with 3D behavior across multiple growth factors, we found that increased membrane protrusion elicited by growth factor stimulation did relate robustly to enhanced 3D migration properties of the MDA-MB-231 and MDA-MB-157 lines. Interestingly, we observed this to be a more reliable relationship than cognate receptor expression or activation levels across these and two additional mammary tumor lines.
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spelling pubmed-33734102012-12-11 2D protrusion but not motility predicts growth factor–induced cancer cell migration in 3D collagen Meyer, Aaron S. Hughes-Alford, Shannon K. Kay, Jennifer E. Castillo, Amalchi Wells, Alan Gertler, Frank B. Lauffenburger, Douglas A. J Cell Biol Research Articles Growth factor–induced migration is a critical step in the dissemination and metastasis of solid tumors. Although differences in properties characterizing cell migration on two-dimensional (2D) substrata versus within three-dimensional (3D) matrices have been noted for particular growth factor stimuli, the 2D approach remains in more common use as an efficient surrogate, especially for high-throughput experiments. We therefore were motivated to investigate which migration properties measured in various 2D assays might be reflective of 3D migratory behavioral responses. We used human triple-negative breast cancer lines stimulated by a panel of receptor tyrosine kinase ligands relevant to mammary carcinoma progression. Whereas 2D migration properties did not correlate well with 3D behavior across multiple growth factors, we found that increased membrane protrusion elicited by growth factor stimulation did relate robustly to enhanced 3D migration properties of the MDA-MB-231 and MDA-MB-157 lines. Interestingly, we observed this to be a more reliable relationship than cognate receptor expression or activation levels across these and two additional mammary tumor lines. The Rockefeller University Press 2012-06-11 /pmc/articles/PMC3373410/ /pubmed/22665521 http://dx.doi.org/10.1083/jcb.201201003 Text en © 2012 Meyer et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Meyer, Aaron S.
Hughes-Alford, Shannon K.
Kay, Jennifer E.
Castillo, Amalchi
Wells, Alan
Gertler, Frank B.
Lauffenburger, Douglas A.
2D protrusion but not motility predicts growth factor–induced cancer cell migration in 3D collagen
title 2D protrusion but not motility predicts growth factor–induced cancer cell migration in 3D collagen
title_full 2D protrusion but not motility predicts growth factor–induced cancer cell migration in 3D collagen
title_fullStr 2D protrusion but not motility predicts growth factor–induced cancer cell migration in 3D collagen
title_full_unstemmed 2D protrusion but not motility predicts growth factor–induced cancer cell migration in 3D collagen
title_short 2D protrusion but not motility predicts growth factor–induced cancer cell migration in 3D collagen
title_sort 2d protrusion but not motility predicts growth factor–induced cancer cell migration in 3d collagen
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3373410/
https://www.ncbi.nlm.nih.gov/pubmed/22665521
http://dx.doi.org/10.1083/jcb.201201003
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