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Detection of Bioactive Exometabolites Produced by the Filamentous Marine Cyanobacterium Geitlerinema sp.
Marine cyanobacteria are noted for their ability to excrete metabolites with biotic properties. This paper focuses on such exometabolites obtained from the culture of the marine filamentous cyanobacterium Geitlerinema sp. strain, their purification and subsequent analyses. By this means the recoveri...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3374093/ https://www.ncbi.nlm.nih.gov/pubmed/22160344 http://dx.doi.org/10.1007/s10126-011-9424-1 |
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author | Caicedo, Nelson H. Kumirska, Jolanta Neumann, Jennifer Stolte, Stefan Thöming, Jorg |
author_facet | Caicedo, Nelson H. Kumirska, Jolanta Neumann, Jennifer Stolte, Stefan Thöming, Jorg |
author_sort | Caicedo, Nelson H. |
collection | PubMed |
description | Marine cyanobacteria are noted for their ability to excrete metabolites with biotic properties. This paper focuses on such exometabolites obtained from the culture of the marine filamentous cyanobacterium Geitlerinema sp. strain, their purification and subsequent analyses. By this means the recoveries of the active compounds, a prerequisite for properly determining their concentration, are quantified here for the first time. We demonstrate a new procedure using Amberlite XAD-1180 resin in combination with the eluent isopropanol for extraction of the culture media and gas chromatography as simplified chemical analysis. This procedure reduced necessary bacteria cultivation time (from 150 to 21 days) at low volumes of culture media (300 mL) required for identification of two selected bioactive compounds: 4,4′-dihydroxybiphenyl and harmane. |
format | Online Article Text |
id | pubmed-3374093 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-33740932012-06-14 Detection of Bioactive Exometabolites Produced by the Filamentous Marine Cyanobacterium Geitlerinema sp. Caicedo, Nelson H. Kumirska, Jolanta Neumann, Jennifer Stolte, Stefan Thöming, Jorg Mar Biotechnol (NY) Original Article Marine cyanobacteria are noted for their ability to excrete metabolites with biotic properties. This paper focuses on such exometabolites obtained from the culture of the marine filamentous cyanobacterium Geitlerinema sp. strain, their purification and subsequent analyses. By this means the recoveries of the active compounds, a prerequisite for properly determining their concentration, are quantified here for the first time. We demonstrate a new procedure using Amberlite XAD-1180 resin in combination with the eluent isopropanol for extraction of the culture media and gas chromatography as simplified chemical analysis. This procedure reduced necessary bacteria cultivation time (from 150 to 21 days) at low volumes of culture media (300 mL) required for identification of two selected bioactive compounds: 4,4′-dihydroxybiphenyl and harmane. Springer-Verlag 2011-12-13 2012 /pmc/articles/PMC3374093/ /pubmed/22160344 http://dx.doi.org/10.1007/s10126-011-9424-1 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Original Article Caicedo, Nelson H. Kumirska, Jolanta Neumann, Jennifer Stolte, Stefan Thöming, Jorg Detection of Bioactive Exometabolites Produced by the Filamentous Marine Cyanobacterium Geitlerinema sp. |
title | Detection of Bioactive Exometabolites Produced by the Filamentous Marine Cyanobacterium Geitlerinema sp. |
title_full | Detection of Bioactive Exometabolites Produced by the Filamentous Marine Cyanobacterium Geitlerinema sp. |
title_fullStr | Detection of Bioactive Exometabolites Produced by the Filamentous Marine Cyanobacterium Geitlerinema sp. |
title_full_unstemmed | Detection of Bioactive Exometabolites Produced by the Filamentous Marine Cyanobacterium Geitlerinema sp. |
title_short | Detection of Bioactive Exometabolites Produced by the Filamentous Marine Cyanobacterium Geitlerinema sp. |
title_sort | detection of bioactive exometabolites produced by the filamentous marine cyanobacterium geitlerinema sp. |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3374093/ https://www.ncbi.nlm.nih.gov/pubmed/22160344 http://dx.doi.org/10.1007/s10126-011-9424-1 |
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