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Mycobacterium tuberculosis and Rifampin Resistance, United Kingdom

The United Kingdom Health Protection Agency Mycobacterium Reference Unit offers a national "Fastrack" molecular service for detecting Mycobacterium tuberculosis complex (MTBC) and rifampin resistance by using the INNO-LiPA Rif.TB assay. We analyzed the service in a routine, nontrial contex...

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Autores principales: Sam, I-Ching, Drobniewski, Francis, More, Philip, Kemp, Melanie, Brown, Timothy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3374436/
https://www.ncbi.nlm.nih.gov/pubmed/16704831
http://dx.doi.org/10.3201/eid1205.041339
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author Sam, I-Ching
Drobniewski, Francis
More, Philip
Kemp, Melanie
Brown, Timothy
author_facet Sam, I-Ching
Drobniewski, Francis
More, Philip
Kemp, Melanie
Brown, Timothy
author_sort Sam, I-Ching
collection PubMed
description The United Kingdom Health Protection Agency Mycobacterium Reference Unit offers a national "Fastrack" molecular service for detecting Mycobacterium tuberculosis complex (MTBC) and rifampin resistance by using the INNO-LiPA Rif.TB assay. We analyzed the service in a routine, nontrial context of 1,997 primary clinical specimens, including 658 nonrespiratory specimens. The overall adjusted concordance, sensitivity, specificity, positive predictive value, and negative predictive value for detecting MTBC were 91.2%, 85.2%, 96.2%, 95.7%, and 86.7%, respectively (unadjusted, 86.7%, 85.2%, 88.2%, 86.9%, and 86.7%), when false-positive samples from patients (n = 83) with a known microbiologic diagnosis of MTBC or patients receiving current or recent antituberculous treatment were excluded. The parameters for detecting rifampin resistance were 99.1%, 95.0%, 99.6%, 92.7%, and 99.7%, respectively. The assay enabled earlier diagnosis of MTBC and rifampin resistance (15.2 days) compared with culture-based techniques (30.7 days).
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spelling pubmed-33744362012-06-28 Mycobacterium tuberculosis and Rifampin Resistance, United Kingdom Sam, I-Ching Drobniewski, Francis More, Philip Kemp, Melanie Brown, Timothy Emerg Infect Dis Research The United Kingdom Health Protection Agency Mycobacterium Reference Unit offers a national "Fastrack" molecular service for detecting Mycobacterium tuberculosis complex (MTBC) and rifampin resistance by using the INNO-LiPA Rif.TB assay. We analyzed the service in a routine, nontrial context of 1,997 primary clinical specimens, including 658 nonrespiratory specimens. The overall adjusted concordance, sensitivity, specificity, positive predictive value, and negative predictive value for detecting MTBC were 91.2%, 85.2%, 96.2%, 95.7%, and 86.7%, respectively (unadjusted, 86.7%, 85.2%, 88.2%, 86.9%, and 86.7%), when false-positive samples from patients (n = 83) with a known microbiologic diagnosis of MTBC or patients receiving current or recent antituberculous treatment were excluded. The parameters for detecting rifampin resistance were 99.1%, 95.0%, 99.6%, 92.7%, and 99.7%, respectively. The assay enabled earlier diagnosis of MTBC and rifampin resistance (15.2 days) compared with culture-based techniques (30.7 days). Centers for Disease Control and Prevention 2006-05 /pmc/articles/PMC3374436/ /pubmed/16704831 http://dx.doi.org/10.3201/eid1205.041339 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research
Sam, I-Ching
Drobniewski, Francis
More, Philip
Kemp, Melanie
Brown, Timothy
Mycobacterium tuberculosis and Rifampin Resistance, United Kingdom
title Mycobacterium tuberculosis and Rifampin Resistance, United Kingdom
title_full Mycobacterium tuberculosis and Rifampin Resistance, United Kingdom
title_fullStr Mycobacterium tuberculosis and Rifampin Resistance, United Kingdom
title_full_unstemmed Mycobacterium tuberculosis and Rifampin Resistance, United Kingdom
title_short Mycobacterium tuberculosis and Rifampin Resistance, United Kingdom
title_sort mycobacterium tuberculosis and rifampin resistance, united kingdom
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3374436/
https://www.ncbi.nlm.nih.gov/pubmed/16704831
http://dx.doi.org/10.3201/eid1205.041339
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