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The inner-mitochondrial distribution of Oxa1 depends on the growth conditions and on the availability of substrates

The Oxa1 protein is a well-conserved integral protein of the inner membrane of mitochondria. It mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane. We investigated the distribution of budding yeast Oxa1 between the two subdomains of the...

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Autores principales: Stoldt, Stefan, Wenzel, Dirk, Hildenbeutel, Markus, Wurm, Christian A., Herrmann, Johannes M., Jakobs, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3374748/
https://www.ncbi.nlm.nih.gov/pubmed/22513091
http://dx.doi.org/10.1091/mbc.E11-06-0538
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author Stoldt, Stefan
Wenzel, Dirk
Hildenbeutel, Markus
Wurm, Christian A.
Herrmann, Johannes M.
Jakobs, Stefan
author_facet Stoldt, Stefan
Wenzel, Dirk
Hildenbeutel, Markus
Wurm, Christian A.
Herrmann, Johannes M.
Jakobs, Stefan
author_sort Stoldt, Stefan
collection PubMed
description The Oxa1 protein is a well-conserved integral protein of the inner membrane of mitochondria. It mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane. We investigated the distribution of budding yeast Oxa1 between the two subdomains of the contiguous inner membrane—the cristae membrane (CM) and the inner boundary membrane (IBM)—under different physiological conditions. We found that under fermentable growth conditions, Oxa1 is enriched in the IBM, whereas under nonfermentable (respiratory) growth conditions, it is predominantly localized in the CM. The enrichment of Oxa1 in the CM requires mitochondrial translation; similarly, deletion of the ribosome-binding domain of Oxa1 prevents an enrichment of Oxa1 in the CM. The predominant localization in the IBM under fermentable growth conditions is prevented by inhibiting mitochondrial protein import. Furthermore, overexpression of the nuclear-encoded Oxa1 substrate Mdl1 shifts the distribution of Oxa1 toward the IBM. Apparently, the availability of nuclear- and mitochondrial-encoded substrates influences the inner-membrane distribution of Oxa1. Our findings show that the distribution of Oxa1 within the inner membrane is dynamic and adapts to different physiological needs.
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spelling pubmed-33747482012-08-30 The inner-mitochondrial distribution of Oxa1 depends on the growth conditions and on the availability of substrates Stoldt, Stefan Wenzel, Dirk Hildenbeutel, Markus Wurm, Christian A. Herrmann, Johannes M. Jakobs, Stefan Mol Biol Cell Articles The Oxa1 protein is a well-conserved integral protein of the inner membrane of mitochondria. It mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane. We investigated the distribution of budding yeast Oxa1 between the two subdomains of the contiguous inner membrane—the cristae membrane (CM) and the inner boundary membrane (IBM)—under different physiological conditions. We found that under fermentable growth conditions, Oxa1 is enriched in the IBM, whereas under nonfermentable (respiratory) growth conditions, it is predominantly localized in the CM. The enrichment of Oxa1 in the CM requires mitochondrial translation; similarly, deletion of the ribosome-binding domain of Oxa1 prevents an enrichment of Oxa1 in the CM. The predominant localization in the IBM under fermentable growth conditions is prevented by inhibiting mitochondrial protein import. Furthermore, overexpression of the nuclear-encoded Oxa1 substrate Mdl1 shifts the distribution of Oxa1 toward the IBM. Apparently, the availability of nuclear- and mitochondrial-encoded substrates influences the inner-membrane distribution of Oxa1. Our findings show that the distribution of Oxa1 within the inner membrane is dynamic and adapts to different physiological needs. The American Society for Cell Biology 2012-06-15 /pmc/articles/PMC3374748/ /pubmed/22513091 http://dx.doi.org/10.1091/mbc.E11-06-0538 Text en © 2012 Stoldt et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Stoldt, Stefan
Wenzel, Dirk
Hildenbeutel, Markus
Wurm, Christian A.
Herrmann, Johannes M.
Jakobs, Stefan
The inner-mitochondrial distribution of Oxa1 depends on the growth conditions and on the availability of substrates
title The inner-mitochondrial distribution of Oxa1 depends on the growth conditions and on the availability of substrates
title_full The inner-mitochondrial distribution of Oxa1 depends on the growth conditions and on the availability of substrates
title_fullStr The inner-mitochondrial distribution of Oxa1 depends on the growth conditions and on the availability of substrates
title_full_unstemmed The inner-mitochondrial distribution of Oxa1 depends on the growth conditions and on the availability of substrates
title_short The inner-mitochondrial distribution of Oxa1 depends on the growth conditions and on the availability of substrates
title_sort inner-mitochondrial distribution of oxa1 depends on the growth conditions and on the availability of substrates
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3374748/
https://www.ncbi.nlm.nih.gov/pubmed/22513091
http://dx.doi.org/10.1091/mbc.E11-06-0538
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