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Identification of a Candidate Proteomic Signature to Discriminate Multipotent and Non-Multipotent Stromal Cells

Bone marrow stromal cell cultures contain multipotent cells that may have therapeutic utility for tissue restoration; however, the identity of the cell that maintains this function remains poorly characterized. We have utilized a unique model of murine bone marrow stroma in combination with liquid c...

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Autores principales: Rosu-Myles, Michael, She, Yi-Min, Fair, Joel, Muradia, Gauri, Mehic, Jelica, Menendez, Pablo, Prasad, Shiv S., Cyr, Terry D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3374805/
https://www.ncbi.nlm.nih.gov/pubmed/22719999
http://dx.doi.org/10.1371/journal.pone.0038954
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author Rosu-Myles, Michael
She, Yi-Min
Fair, Joel
Muradia, Gauri
Mehic, Jelica
Menendez, Pablo
Prasad, Shiv S.
Cyr, Terry D.
author_facet Rosu-Myles, Michael
She, Yi-Min
Fair, Joel
Muradia, Gauri
Mehic, Jelica
Menendez, Pablo
Prasad, Shiv S.
Cyr, Terry D.
author_sort Rosu-Myles, Michael
collection PubMed
description Bone marrow stromal cell cultures contain multipotent cells that may have therapeutic utility for tissue restoration; however, the identity of the cell that maintains this function remains poorly characterized. We have utilized a unique model of murine bone marrow stroma in combination with liquid chromatography mass spectrometry to compare the nuclear, cytoplasmic and membrane associated proteomes of multipotent (MSC) (CD105+) and non-multipotent (CD105−) stromal cells. Among the 25 most reliably identified proteins, 10 were verified by both real-time PCR and Western Blot to be highly enriched, in CD105+ cells and were members of distinct biological pathways and functional networks. Five of these proteins were also identified as potentially expressed in human MSC derived from both standard and serum free human stromal cultures. The quantitative amount of each protein identified in human stromal cells was only minimally affected by media conditions but varied highly between bone marrow donors. This study provides further evidence of heterogeneity among cultured bone marrow stromal cells and identifies potential candidate proteins that may prove useful for identifying and quantifying both murine and human MSC in vitro.
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spelling pubmed-33748052012-06-20 Identification of a Candidate Proteomic Signature to Discriminate Multipotent and Non-Multipotent Stromal Cells Rosu-Myles, Michael She, Yi-Min Fair, Joel Muradia, Gauri Mehic, Jelica Menendez, Pablo Prasad, Shiv S. Cyr, Terry D. PLoS One Research Article Bone marrow stromal cell cultures contain multipotent cells that may have therapeutic utility for tissue restoration; however, the identity of the cell that maintains this function remains poorly characterized. We have utilized a unique model of murine bone marrow stroma in combination with liquid chromatography mass spectrometry to compare the nuclear, cytoplasmic and membrane associated proteomes of multipotent (MSC) (CD105+) and non-multipotent (CD105−) stromal cells. Among the 25 most reliably identified proteins, 10 were verified by both real-time PCR and Western Blot to be highly enriched, in CD105+ cells and were members of distinct biological pathways and functional networks. Five of these proteins were also identified as potentially expressed in human MSC derived from both standard and serum free human stromal cultures. The quantitative amount of each protein identified in human stromal cells was only minimally affected by media conditions but varied highly between bone marrow donors. This study provides further evidence of heterogeneity among cultured bone marrow stromal cells and identifies potential candidate proteins that may prove useful for identifying and quantifying both murine and human MSC in vitro. Public Library of Science 2012-06-13 /pmc/articles/PMC3374805/ /pubmed/22719999 http://dx.doi.org/10.1371/journal.pone.0038954 Text en Rosu-Myles et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Rosu-Myles, Michael
She, Yi-Min
Fair, Joel
Muradia, Gauri
Mehic, Jelica
Menendez, Pablo
Prasad, Shiv S.
Cyr, Terry D.
Identification of a Candidate Proteomic Signature to Discriminate Multipotent and Non-Multipotent Stromal Cells
title Identification of a Candidate Proteomic Signature to Discriminate Multipotent and Non-Multipotent Stromal Cells
title_full Identification of a Candidate Proteomic Signature to Discriminate Multipotent and Non-Multipotent Stromal Cells
title_fullStr Identification of a Candidate Proteomic Signature to Discriminate Multipotent and Non-Multipotent Stromal Cells
title_full_unstemmed Identification of a Candidate Proteomic Signature to Discriminate Multipotent and Non-Multipotent Stromal Cells
title_short Identification of a Candidate Proteomic Signature to Discriminate Multipotent and Non-Multipotent Stromal Cells
title_sort identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3374805/
https://www.ncbi.nlm.nih.gov/pubmed/22719999
http://dx.doi.org/10.1371/journal.pone.0038954
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