Enhancement of crystallization with nucleotide ligands identified by dye-ligand affinity chromatography

Ligands interacting with Mycobacterium tuberculosis recombinant proteins were identified through use of the ability of Cibacron Blue F3GA dye to interact with nucleoside/nucleotide binding proteins, and the effects of these ligands on crystallization were examined. Co-crystallization with ligands en...

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Detalles Bibliográficos
Autores principales: Kim, Heungbok, Webster, Cecelia, Roberts, Justin K. M., Kositsawat, Juthamas, Hung, Li-Wei, Terwilliger, Thomas C., Kim, Chang-Yub
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3375012/
https://www.ncbi.nlm.nih.gov/pubmed/22286688
http://dx.doi.org/10.1007/s10969-012-9124-8
Descripción
Sumario:Ligands interacting with Mycobacterium tuberculosis recombinant proteins were identified through use of the ability of Cibacron Blue F3GA dye to interact with nucleoside/nucleotide binding proteins, and the effects of these ligands on crystallization were examined. Co-crystallization with ligands enhanced crystallization and enabled X-ray diffraction data to be collected to a resolution of at least 2.7 Å for 5 of 10 proteins tested. Additionally, clues about individual proteins’ functions were obtained from their interactions with each of a panel of ligands. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10969-012-9124-8) contains supplementary material, which is available to authorized users.

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