Enhancement of crystallization with nucleotide ligands identified by dye-ligand affinity chromatography

Ligands interacting with Mycobacterium tuberculosis recombinant proteins were identified through use of the ability of Cibacron Blue F3GA dye to interact with nucleoside/nucleotide binding proteins, and the effects of these ligands on crystallization were examined. Co-crystallization with ligands en...

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Autores principales: Kim, Heungbok, Webster, Cecelia, Roberts, Justin K. M., Kositsawat, Juthamas, Hung, Li-Wei, Terwilliger, Thomas C., Kim, Chang-Yub
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3375012/
https://www.ncbi.nlm.nih.gov/pubmed/22286688
http://dx.doi.org/10.1007/s10969-012-9124-8
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author Kim, Heungbok
Webster, Cecelia
Roberts, Justin K. M.
Kositsawat, Juthamas
Hung, Li-Wei
Terwilliger, Thomas C.
Kim, Chang-Yub
author_facet Kim, Heungbok
Webster, Cecelia
Roberts, Justin K. M.
Kositsawat, Juthamas
Hung, Li-Wei
Terwilliger, Thomas C.
Kim, Chang-Yub
author_sort Kim, Heungbok
collection PubMed
description Ligands interacting with Mycobacterium tuberculosis recombinant proteins were identified through use of the ability of Cibacron Blue F3GA dye to interact with nucleoside/nucleotide binding proteins, and the effects of these ligands on crystallization were examined. Co-crystallization with ligands enhanced crystallization and enabled X-ray diffraction data to be collected to a resolution of at least 2.7 Å for 5 of 10 proteins tested. Additionally, clues about individual proteins’ functions were obtained from their interactions with each of a panel of ligands. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10969-012-9124-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-33750122012-06-18 Enhancement of crystallization with nucleotide ligands identified by dye-ligand affinity chromatography Kim, Heungbok Webster, Cecelia Roberts, Justin K. M. Kositsawat, Juthamas Hung, Li-Wei Terwilliger, Thomas C. Kim, Chang-Yub J Struct Funct Genomics Article Ligands interacting with Mycobacterium tuberculosis recombinant proteins were identified through use of the ability of Cibacron Blue F3GA dye to interact with nucleoside/nucleotide binding proteins, and the effects of these ligands on crystallization were examined. Co-crystallization with ligands enhanced crystallization and enabled X-ray diffraction data to be collected to a resolution of at least 2.7 Å for 5 of 10 proteins tested. Additionally, clues about individual proteins’ functions were obtained from their interactions with each of a panel of ligands. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10969-012-9124-8) contains supplementary material, which is available to authorized users. Springer Netherlands 2012-01-28 2012 /pmc/articles/PMC3375012/ /pubmed/22286688 http://dx.doi.org/10.1007/s10969-012-9124-8 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Kim, Heungbok
Webster, Cecelia
Roberts, Justin K. M.
Kositsawat, Juthamas
Hung, Li-Wei
Terwilliger, Thomas C.
Kim, Chang-Yub
Enhancement of crystallization with nucleotide ligands identified by dye-ligand affinity chromatography
title Enhancement of crystallization with nucleotide ligands identified by dye-ligand affinity chromatography
title_full Enhancement of crystallization with nucleotide ligands identified by dye-ligand affinity chromatography
title_fullStr Enhancement of crystallization with nucleotide ligands identified by dye-ligand affinity chromatography
title_full_unstemmed Enhancement of crystallization with nucleotide ligands identified by dye-ligand affinity chromatography
title_short Enhancement of crystallization with nucleotide ligands identified by dye-ligand affinity chromatography
title_sort enhancement of crystallization with nucleotide ligands identified by dye-ligand affinity chromatography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3375012/
https://www.ncbi.nlm.nih.gov/pubmed/22286688
http://dx.doi.org/10.1007/s10969-012-9124-8
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