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A Pilot Study for the Neuroprotective Effect of Gongjin-dan on Transient Middle Cerebral Artery Occlusion-Induced Ischemic Rat Brain

In this study, we investigated whether gongjin-dan improves functional recovery and has neuroprotective effects on reducing the infarct volume after transient middle cerebral artery occlusion (MCAo). Infarct volume was measured using TTC staining and glucose utilization by F-18 FDG PET. Functional i...

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Autores principales: Sunwoo, Yun-Young, Park, Sang In, Chung, Yong-An, Lee, Jisoo, Park, Moon-Seo, Jang, Kyung-Sool, Maeng, Lee-So, Jang, Dong-Kyu, Im, Ruth, Jung, Yu Jin, Park, Soon A., Kang, Eun-Sun, Kim, Min-Wook, Han, Young-Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3375177/
https://www.ncbi.nlm.nih.gov/pubmed/22719787
http://dx.doi.org/10.1155/2012/682720
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author Sunwoo, Yun-Young
Park, Sang In
Chung, Yong-An
Lee, Jisoo
Park, Moon-Seo
Jang, Kyung-Sool
Maeng, Lee-So
Jang, Dong-Kyu
Im, Ruth
Jung, Yu Jin
Park, Soon A.
Kang, Eun-Sun
Kim, Min-Wook
Han, Young-Min
author_facet Sunwoo, Yun-Young
Park, Sang In
Chung, Yong-An
Lee, Jisoo
Park, Moon-Seo
Jang, Kyung-Sool
Maeng, Lee-So
Jang, Dong-Kyu
Im, Ruth
Jung, Yu Jin
Park, Soon A.
Kang, Eun-Sun
Kim, Min-Wook
Han, Young-Min
author_sort Sunwoo, Yun-Young
collection PubMed
description In this study, we investigated whether gongjin-dan improves functional recovery and has neuroprotective effects on reducing the infarct volume after transient middle cerebral artery occlusion (MCAo). Infarct volume was measured using TTC staining and glucose utilization by F-18 FDG PET. Functional improvement was evaluated with the Rota-rod, treadmill, Garcia score test, and adhesive removal test. At 14 days after MCAo, neuronal cell survival, astrocytes expansion, and apoptosis were assessed by immunohistofluorescence staining in the peri-infarct region. Also, the expression of neurotrophic factors and inflammatory cytokines such as VEGF, BDNF, Cox-2, TNF-α, IL-1β, and IL-1α was measured in ischemic hemisphere regions. The gongjin-dan-treated group showed both reduced infarct volume and increased glucose utilization. Behavior tests demonstrated a significant improvement compared to the control. Also in the gongjin-dan treated group, NeuN-positive cells were increased and number of astrocytes, microglia, and apoptotic cells was significantly decreased compared with the control group in the ischemic peri-infarct area. Furthermore, the expression of VEGF and BDNF was increased and level of Cox-2, TNF-α, IL-1β, and IL-1α was decreased. These results suggest that gongjin-dan may improve functional outcome through the rapid restoration of metabolism and can be considered as a potential neuroprotective agent.
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spelling pubmed-33751772012-06-20 A Pilot Study for the Neuroprotective Effect of Gongjin-dan on Transient Middle Cerebral Artery Occlusion-Induced Ischemic Rat Brain Sunwoo, Yun-Young Park, Sang In Chung, Yong-An Lee, Jisoo Park, Moon-Seo Jang, Kyung-Sool Maeng, Lee-So Jang, Dong-Kyu Im, Ruth Jung, Yu Jin Park, Soon A. Kang, Eun-Sun Kim, Min-Wook Han, Young-Min Evid Based Complement Alternat Med Research Article In this study, we investigated whether gongjin-dan improves functional recovery and has neuroprotective effects on reducing the infarct volume after transient middle cerebral artery occlusion (MCAo). Infarct volume was measured using TTC staining and glucose utilization by F-18 FDG PET. Functional improvement was evaluated with the Rota-rod, treadmill, Garcia score test, and adhesive removal test. At 14 days after MCAo, neuronal cell survival, astrocytes expansion, and apoptosis were assessed by immunohistofluorescence staining in the peri-infarct region. Also, the expression of neurotrophic factors and inflammatory cytokines such as VEGF, BDNF, Cox-2, TNF-α, IL-1β, and IL-1α was measured in ischemic hemisphere regions. The gongjin-dan-treated group showed both reduced infarct volume and increased glucose utilization. Behavior tests demonstrated a significant improvement compared to the control. Also in the gongjin-dan treated group, NeuN-positive cells were increased and number of astrocytes, microglia, and apoptotic cells was significantly decreased compared with the control group in the ischemic peri-infarct area. Furthermore, the expression of VEGF and BDNF was increased and level of Cox-2, TNF-α, IL-1β, and IL-1α was decreased. These results suggest that gongjin-dan may improve functional outcome through the rapid restoration of metabolism and can be considered as a potential neuroprotective agent. Hindawi Publishing Corporation 2012 2012-06-06 /pmc/articles/PMC3375177/ /pubmed/22719787 http://dx.doi.org/10.1155/2012/682720 Text en Copyright © 2012 Yun-Young Sunwoo et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sunwoo, Yun-Young
Park, Sang In
Chung, Yong-An
Lee, Jisoo
Park, Moon-Seo
Jang, Kyung-Sool
Maeng, Lee-So
Jang, Dong-Kyu
Im, Ruth
Jung, Yu Jin
Park, Soon A.
Kang, Eun-Sun
Kim, Min-Wook
Han, Young-Min
A Pilot Study for the Neuroprotective Effect of Gongjin-dan on Transient Middle Cerebral Artery Occlusion-Induced Ischemic Rat Brain
title A Pilot Study for the Neuroprotective Effect of Gongjin-dan on Transient Middle Cerebral Artery Occlusion-Induced Ischemic Rat Brain
title_full A Pilot Study for the Neuroprotective Effect of Gongjin-dan on Transient Middle Cerebral Artery Occlusion-Induced Ischemic Rat Brain
title_fullStr A Pilot Study for the Neuroprotective Effect of Gongjin-dan on Transient Middle Cerebral Artery Occlusion-Induced Ischemic Rat Brain
title_full_unstemmed A Pilot Study for the Neuroprotective Effect of Gongjin-dan on Transient Middle Cerebral Artery Occlusion-Induced Ischemic Rat Brain
title_short A Pilot Study for the Neuroprotective Effect of Gongjin-dan on Transient Middle Cerebral Artery Occlusion-Induced Ischemic Rat Brain
title_sort pilot study for the neuroprotective effect of gongjin-dan on transient middle cerebral artery occlusion-induced ischemic rat brain
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3375177/
https://www.ncbi.nlm.nih.gov/pubmed/22719787
http://dx.doi.org/10.1155/2012/682720
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