IgG Avidity ELISA Test for Diagnosis of Acute Toxoplasmosis in Humans

Serum samples, 100 in the total number, were collected from different laboratories in Tehran, Iran and tested for anti-Toxoplasma specific IgG and IgM antibodies using indirect immunofluorescent antibody test (IFAT). Using the IgG (chronic) and IgM (acute) positive samples, the IgG avidity test was...

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Autores principales: Rahbari, Amir Hossien, Keshavarz, Hossien, Shojaee, Saeedeh, Mohebali, Mehdi, Rezaeian, Mostafa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Parasitology 2012
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3375462/
https://www.ncbi.nlm.nih.gov/pubmed/22711919
http://dx.doi.org/10.3347/kjp.2012.50.2.99
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author Rahbari, Amir Hossien
Keshavarz, Hossien
Shojaee, Saeedeh
Mohebali, Mehdi
Rezaeian, Mostafa
author_facet Rahbari, Amir Hossien
Keshavarz, Hossien
Shojaee, Saeedeh
Mohebali, Mehdi
Rezaeian, Mostafa
author_sort Rahbari, Amir Hossien
collection PubMed
description Serum samples, 100 in the total number, were collected from different laboratories in Tehran, Iran and tested for anti-Toxoplasma specific IgG and IgM antibodies using indirect immunofluorescent antibody test (IFAT). Using the IgG (chronic) and IgM (acute) positive samples, the IgG avidity test was performed by ELISA in duplicate rows of 96-well microtiter plates. One row was washed with 6 M urea and the other with PBS (pH 7.2), then the avidity index (AI) was calculated. Sixteen out of 18 (88.9%) sera with acute toxoplasmosis showed low avidity levels (AI≤50), and 76 out of 82 (92.7%) sera in chronic phase of infection showed high avidity index (AI>60). Six sera had borderline ranges of AI. The results showed that the IgG avidity test by ELISA could distinguish the acute and chronic stages of toxoplasmosis in humans.
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spelling pubmed-33754622012-06-18 IgG Avidity ELISA Test for Diagnosis of Acute Toxoplasmosis in Humans Rahbari, Amir Hossien Keshavarz, Hossien Shojaee, Saeedeh Mohebali, Mehdi Rezaeian, Mostafa Korean J Parasitol Original Article Serum samples, 100 in the total number, were collected from different laboratories in Tehran, Iran and tested for anti-Toxoplasma specific IgG and IgM antibodies using indirect immunofluorescent antibody test (IFAT). Using the IgG (chronic) and IgM (acute) positive samples, the IgG avidity test was performed by ELISA in duplicate rows of 96-well microtiter plates. One row was washed with 6 M urea and the other with PBS (pH 7.2), then the avidity index (AI) was calculated. Sixteen out of 18 (88.9%) sera with acute toxoplasmosis showed low avidity levels (AI≤50), and 76 out of 82 (92.7%) sera in chronic phase of infection showed high avidity index (AI>60). Six sera had borderline ranges of AI. The results showed that the IgG avidity test by ELISA could distinguish the acute and chronic stages of toxoplasmosis in humans. The Korean Society for Parasitology 2012-06 2012-05-24 /pmc/articles/PMC3375462/ /pubmed/22711919 http://dx.doi.org/10.3347/kjp.2012.50.2.99 Text en © 2012, Korean Society for Parasitology http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Rahbari, Amir Hossien
Keshavarz, Hossien
Shojaee, Saeedeh
Mohebali, Mehdi
Rezaeian, Mostafa
IgG Avidity ELISA Test for Diagnosis of Acute Toxoplasmosis in Humans
title IgG Avidity ELISA Test for Diagnosis of Acute Toxoplasmosis in Humans
title_full IgG Avidity ELISA Test for Diagnosis of Acute Toxoplasmosis in Humans
title_fullStr IgG Avidity ELISA Test for Diagnosis of Acute Toxoplasmosis in Humans
title_full_unstemmed IgG Avidity ELISA Test for Diagnosis of Acute Toxoplasmosis in Humans
title_short IgG Avidity ELISA Test for Diagnosis of Acute Toxoplasmosis in Humans
title_sort igg avidity elisa test for diagnosis of acute toxoplasmosis in humans
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3375462/
https://www.ncbi.nlm.nih.gov/pubmed/22711919
http://dx.doi.org/10.3347/kjp.2012.50.2.99
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