Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

The efficient generation of genetic diversity represents an invaluable molecular tool that can be used to label DNA synthesis, to create unique molecular signatures, or to evolve proteins in the laboratory. Here, we present a protocol that allows the generation of large (>10(11)) mutant libraries...

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Detalles Bibliográficos
Autores principales: Troll, Chris, Alexander, David, Allen, Jennifer, Marquette, Jacob, Camps, Manel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2011
Materias:
Genetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3376869/
https://www.ncbi.nlm.nih.gov/pubmed/21445044
http://dx.doi.org/10.3791/2505

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3376869/
https://www.ncbi.nlm.nih.gov/pubmed/21445044
http://dx.doi.org/10.3791/2505

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