Quantitative tracking of T cell clones after haematopoietic stem cell transplantation

Autologous haematopoietic stem cell transplantation is highly efficient for the treatment of systemic autoimmune diseases, but its consequences for the immune system remain poorly understood. Here, we describe an optimized RNA-based technology for unbiased amplification of T cell receptor beta-chain...

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Detalles Bibliográficos
Autores principales: Mamedov, Ilgar Z, Britanova, Olga V, Bolotin, Dmitriy A, Chkalina, Anna V, Staroverov, Dmitriy B, Zvyagin, Ivan V, Kotlobay, Alexey A, Turchaninova, Maria A, Fedorenko, Denis A, Novik, Andrew A, Sharonov, George V, Lukyanov, Sergey, Chudakov, Dmitriy M, Lebedev, Yuri B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: WILEY-VCH Verlag 2011
Materias:
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3377069/
https://www.ncbi.nlm.nih.gov/pubmed/21374820
http://dx.doi.org/10.1002/emmm.201100129
Descripción
Sumario:Autologous haematopoietic stem cell transplantation is highly efficient for the treatment of systemic autoimmune diseases, but its consequences for the immune system remain poorly understood. Here, we describe an optimized RNA-based technology for unbiased amplification of T cell receptor beta-chain libraries and use it to perform the first detailed, quantitative tracking of T cell clones during 10 months after transplantation. We show that multiple clones survive the procedure, contribute to the immune response to activated infections, and form a new skewed and stable T cell receptor repertoire.

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