Quantitative tracking of T cell clones after haematopoietic stem cell transplantation

Autologous haematopoietic stem cell transplantation is highly efficient for the treatment of systemic autoimmune diseases, but its consequences for the immune system remain poorly understood. Here, we describe an optimized RNA-based technology for unbiased amplification of T cell receptor beta-chain...

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Autores principales: Mamedov, Ilgar Z, Britanova, Olga V, Bolotin, Dmitriy A, Chkalina, Anna V, Staroverov, Dmitriy B, Zvyagin, Ivan V, Kotlobay, Alexey A, Turchaninova, Maria A, Fedorenko, Denis A, Novik, Andrew A, Sharonov, George V, Lukyanov, Sergey, Chudakov, Dmitriy M, Lebedev, Yuri B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: WILEY-VCH Verlag 2011
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3377069/
https://www.ncbi.nlm.nih.gov/pubmed/21374820
http://dx.doi.org/10.1002/emmm.201100129
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author Mamedov, Ilgar Z
Britanova, Olga V
Bolotin, Dmitriy A
Chkalina, Anna V
Staroverov, Dmitriy B
Zvyagin, Ivan V
Kotlobay, Alexey A
Turchaninova, Maria A
Fedorenko, Denis A
Novik, Andrew A
Sharonov, George V
Lukyanov, Sergey
Chudakov, Dmitriy M
Lebedev, Yuri B
author_facet Mamedov, Ilgar Z
Britanova, Olga V
Bolotin, Dmitriy A
Chkalina, Anna V
Staroverov, Dmitriy B
Zvyagin, Ivan V
Kotlobay, Alexey A
Turchaninova, Maria A
Fedorenko, Denis A
Novik, Andrew A
Sharonov, George V
Lukyanov, Sergey
Chudakov, Dmitriy M
Lebedev, Yuri B
author_sort Mamedov, Ilgar Z
collection PubMed
description Autologous haematopoietic stem cell transplantation is highly efficient for the treatment of systemic autoimmune diseases, but its consequences for the immune system remain poorly understood. Here, we describe an optimized RNA-based technology for unbiased amplification of T cell receptor beta-chain libraries and use it to perform the first detailed, quantitative tracking of T cell clones during 10 months after transplantation. We show that multiple clones survive the procedure, contribute to the immune response to activated infections, and form a new skewed and stable T cell receptor repertoire.
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spelling pubmed-33770692012-09-17 Quantitative tracking of T cell clones after haematopoietic stem cell transplantation Mamedov, Ilgar Z Britanova, Olga V Bolotin, Dmitriy A Chkalina, Anna V Staroverov, Dmitriy B Zvyagin, Ivan V Kotlobay, Alexey A Turchaninova, Maria A Fedorenko, Denis A Novik, Andrew A Sharonov, George V Lukyanov, Sergey Chudakov, Dmitriy M Lebedev, Yuri B EMBO Mol Med Report Autologous haematopoietic stem cell transplantation is highly efficient for the treatment of systemic autoimmune diseases, but its consequences for the immune system remain poorly understood. Here, we describe an optimized RNA-based technology for unbiased amplification of T cell receptor beta-chain libraries and use it to perform the first detailed, quantitative tracking of T cell clones during 10 months after transplantation. We show that multiple clones survive the procedure, contribute to the immune response to activated infections, and form a new skewed and stable T cell receptor repertoire. WILEY-VCH Verlag 2011-04 /pmc/articles/PMC3377069/ /pubmed/21374820 http://dx.doi.org/10.1002/emmm.201100129 Text en Copyright © 2011 EMBO Molecular Medicine
spellingShingle Report
Mamedov, Ilgar Z
Britanova, Olga V
Bolotin, Dmitriy A
Chkalina, Anna V
Staroverov, Dmitriy B
Zvyagin, Ivan V
Kotlobay, Alexey A
Turchaninova, Maria A
Fedorenko, Denis A
Novik, Andrew A
Sharonov, George V
Lukyanov, Sergey
Chudakov, Dmitriy M
Lebedev, Yuri B
Quantitative tracking of T cell clones after haematopoietic stem cell transplantation
title Quantitative tracking of T cell clones after haematopoietic stem cell transplantation
title_full Quantitative tracking of T cell clones after haematopoietic stem cell transplantation
title_fullStr Quantitative tracking of T cell clones after haematopoietic stem cell transplantation
title_full_unstemmed Quantitative tracking of T cell clones after haematopoietic stem cell transplantation
title_short Quantitative tracking of T cell clones after haematopoietic stem cell transplantation
title_sort quantitative tracking of t cell clones after haematopoietic stem cell transplantation
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3377069/
https://www.ncbi.nlm.nih.gov/pubmed/21374820
http://dx.doi.org/10.1002/emmm.201100129
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