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In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell
BACKGROUND: Strobilanthes crispus has been traditionally used as antidiabetic, anticancer, diuretic, antilytic and laxative agent. However, cytotoxicity and antiproliferative effect of S. crispus is still unclear. RESULTS: Strobilanthes cripus was able to reduce cell viability and proliferation in M...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3377542/ https://www.ncbi.nlm.nih.gov/pubmed/22471785 http://dx.doi.org/10.1186/1472-6882-12-35 |
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author | Chong, Hueh Zan Rahmat, Asmah Yeap, Swee Keong Md Akim, Abdah Alitheen, Noorjahan Banu Othman, Fauziah Gwendoline-Ee, Cheng Lian |
author_facet | Chong, Hueh Zan Rahmat, Asmah Yeap, Swee Keong Md Akim, Abdah Alitheen, Noorjahan Banu Othman, Fauziah Gwendoline-Ee, Cheng Lian |
author_sort | Chong, Hueh Zan |
collection | PubMed |
description | BACKGROUND: Strobilanthes crispus has been traditionally used as antidiabetic, anticancer, diuretic, antilytic and laxative agent. However, cytotoxicity and antiproliferative effect of S. crispus is still unclear. RESULTS: Strobilanthes cripus was able to reduce cell viability and proliferation in MTT and BrdU assays. Both cell cycle progression and Tunel assay suggested that IC(50 )of S. crispus ethanol extract induced sub-G1 cell cycle phase, and DNA fragmentation. On the other hand, translocation of mitochondria cytochrome c release, induction of caspase 3/7 and p53 while suppress XIAP on treated MCF-7 cell were also observed in this study. CONCLUSION: Our findings suggest that S. crispus ethanol extract induced apoptosis and DNA fragmentation on hormone dependent breast cancer cell line MCF-7 via mitochondria dependent p53 apoptosis pathway. |
format | Online Article Text |
id | pubmed-3377542 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-33775422012-06-19 In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell Chong, Hueh Zan Rahmat, Asmah Yeap, Swee Keong Md Akim, Abdah Alitheen, Noorjahan Banu Othman, Fauziah Gwendoline-Ee, Cheng Lian BMC Complement Altern Med Research Article BACKGROUND: Strobilanthes crispus has been traditionally used as antidiabetic, anticancer, diuretic, antilytic and laxative agent. However, cytotoxicity and antiproliferative effect of S. crispus is still unclear. RESULTS: Strobilanthes cripus was able to reduce cell viability and proliferation in MTT and BrdU assays. Both cell cycle progression and Tunel assay suggested that IC(50 )of S. crispus ethanol extract induced sub-G1 cell cycle phase, and DNA fragmentation. On the other hand, translocation of mitochondria cytochrome c release, induction of caspase 3/7 and p53 while suppress XIAP on treated MCF-7 cell were also observed in this study. CONCLUSION: Our findings suggest that S. crispus ethanol extract induced apoptosis and DNA fragmentation on hormone dependent breast cancer cell line MCF-7 via mitochondria dependent p53 apoptosis pathway. BioMed Central 2012-04-04 /pmc/articles/PMC3377542/ /pubmed/22471785 http://dx.doi.org/10.1186/1472-6882-12-35 Text en Copyright ©2012 Chong et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Chong, Hueh Zan Rahmat, Asmah Yeap, Swee Keong Md Akim, Abdah Alitheen, Noorjahan Banu Othman, Fauziah Gwendoline-Ee, Cheng Lian In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell |
title | In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell |
title_full | In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell |
title_fullStr | In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell |
title_full_unstemmed | In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell |
title_short | In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell |
title_sort | in vitro cytotoxicity of strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma mcf-7 cell |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3377542/ https://www.ncbi.nlm.nih.gov/pubmed/22471785 http://dx.doi.org/10.1186/1472-6882-12-35 |
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