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Human Umbilical Cord Blood-Derived CD34(+) Cells Reverse Osteoporosis in NOD/SCID Mice by Altering Osteoblastic and Osteoclastic Activities

BACKGROUND: Osteoporosis is a bone disorder associated with loss of bone mineral density and micro architecture. A balance of osteoblasts and osteoclasts activities maintains bone homeostasis. Increased bone loss due to increased osteoclast and decreased osteoblast activities is considered as an und...

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Autores principales: Aggarwal, Reeva, Lu, Jingwei, Kanji, Suman, Joseph, Matthew, Das, Manjusri, Noble, Garrett J., McMichael, Brooke K., Agarwal, Sudha, Hart, Richard T., Sun, Zongyang, Lee, Beth S., Rosol, Thomas J., Jackson, Rebecca, Mao, Hai-Quan, Pompili, Vincent J., Das, Hiranmoy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3377665/
https://www.ncbi.nlm.nih.gov/pubmed/22724005
http://dx.doi.org/10.1371/journal.pone.0039365
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author Aggarwal, Reeva
Lu, Jingwei
Kanji, Suman
Joseph, Matthew
Das, Manjusri
Noble, Garrett J.
McMichael, Brooke K.
Agarwal, Sudha
Hart, Richard T.
Sun, Zongyang
Lee, Beth S.
Rosol, Thomas J.
Jackson, Rebecca
Mao, Hai-Quan
Pompili, Vincent J.
Das, Hiranmoy
author_facet Aggarwal, Reeva
Lu, Jingwei
Kanji, Suman
Joseph, Matthew
Das, Manjusri
Noble, Garrett J.
McMichael, Brooke K.
Agarwal, Sudha
Hart, Richard T.
Sun, Zongyang
Lee, Beth S.
Rosol, Thomas J.
Jackson, Rebecca
Mao, Hai-Quan
Pompili, Vincent J.
Das, Hiranmoy
author_sort Aggarwal, Reeva
collection PubMed
description BACKGROUND: Osteoporosis is a bone disorder associated with loss of bone mineral density and micro architecture. A balance of osteoblasts and osteoclasts activities maintains bone homeostasis. Increased bone loss due to increased osteoclast and decreased osteoblast activities is considered as an underlying cause of osteoporosis. METHODS AND FINDINGS: The cures for osteoporosis are limited, consequently the potential of CD34+ cell therapies is currently being considered. We developed a nanofiber-based expansion technology to obtain adequate numbers of CD34(+) cells isolated from human umbilical cord blood, for therapeutic applications. Herein, we show that CD34(+) cells could be differentiated into osteoblastic lineage, in vitro. Systemically delivered CD34(+) cells home to the bone marrow and significantly improve bone deposition, bone mineral density and bone micro-architecture in osteoporotic mice. The elevated levels of osteocalcin, IL-10, GM-CSF, and decreased levels of MCP-1 in serum parallel the improvements in bone micro-architecture. Furthermore, CD34(+) cells improved osteoblast activity and concurrently impaired osteoclast differentiation, maturation and functionality. CONCLUSIONS: These findings demonstrate a novel approach utilizing nanofiber-expanded CD34(+) cells as a therapeutic application for the treatment of osteoporosis.
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spelling pubmed-33776652012-06-21 Human Umbilical Cord Blood-Derived CD34(+) Cells Reverse Osteoporosis in NOD/SCID Mice by Altering Osteoblastic and Osteoclastic Activities Aggarwal, Reeva Lu, Jingwei Kanji, Suman Joseph, Matthew Das, Manjusri Noble, Garrett J. McMichael, Brooke K. Agarwal, Sudha Hart, Richard T. Sun, Zongyang Lee, Beth S. Rosol, Thomas J. Jackson, Rebecca Mao, Hai-Quan Pompili, Vincent J. Das, Hiranmoy PLoS One Research Article BACKGROUND: Osteoporosis is a bone disorder associated with loss of bone mineral density and micro architecture. A balance of osteoblasts and osteoclasts activities maintains bone homeostasis. Increased bone loss due to increased osteoclast and decreased osteoblast activities is considered as an underlying cause of osteoporosis. METHODS AND FINDINGS: The cures for osteoporosis are limited, consequently the potential of CD34+ cell therapies is currently being considered. We developed a nanofiber-based expansion technology to obtain adequate numbers of CD34(+) cells isolated from human umbilical cord blood, for therapeutic applications. Herein, we show that CD34(+) cells could be differentiated into osteoblastic lineage, in vitro. Systemically delivered CD34(+) cells home to the bone marrow and significantly improve bone deposition, bone mineral density and bone micro-architecture in osteoporotic mice. The elevated levels of osteocalcin, IL-10, GM-CSF, and decreased levels of MCP-1 in serum parallel the improvements in bone micro-architecture. Furthermore, CD34(+) cells improved osteoblast activity and concurrently impaired osteoclast differentiation, maturation and functionality. CONCLUSIONS: These findings demonstrate a novel approach utilizing nanofiber-expanded CD34(+) cells as a therapeutic application for the treatment of osteoporosis. Public Library of Science 2012-06-18 /pmc/articles/PMC3377665/ /pubmed/22724005 http://dx.doi.org/10.1371/journal.pone.0039365 Text en Aggarwal et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Aggarwal, Reeva
Lu, Jingwei
Kanji, Suman
Joseph, Matthew
Das, Manjusri
Noble, Garrett J.
McMichael, Brooke K.
Agarwal, Sudha
Hart, Richard T.
Sun, Zongyang
Lee, Beth S.
Rosol, Thomas J.
Jackson, Rebecca
Mao, Hai-Quan
Pompili, Vincent J.
Das, Hiranmoy
Human Umbilical Cord Blood-Derived CD34(+) Cells Reverse Osteoporosis in NOD/SCID Mice by Altering Osteoblastic and Osteoclastic Activities
title Human Umbilical Cord Blood-Derived CD34(+) Cells Reverse Osteoporosis in NOD/SCID Mice by Altering Osteoblastic and Osteoclastic Activities
title_full Human Umbilical Cord Blood-Derived CD34(+) Cells Reverse Osteoporosis in NOD/SCID Mice by Altering Osteoblastic and Osteoclastic Activities
title_fullStr Human Umbilical Cord Blood-Derived CD34(+) Cells Reverse Osteoporosis in NOD/SCID Mice by Altering Osteoblastic and Osteoclastic Activities
title_full_unstemmed Human Umbilical Cord Blood-Derived CD34(+) Cells Reverse Osteoporosis in NOD/SCID Mice by Altering Osteoblastic and Osteoclastic Activities
title_short Human Umbilical Cord Blood-Derived CD34(+) Cells Reverse Osteoporosis in NOD/SCID Mice by Altering Osteoblastic and Osteoclastic Activities
title_sort human umbilical cord blood-derived cd34(+) cells reverse osteoporosis in nod/scid mice by altering osteoblastic and osteoclastic activities
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3377665/
https://www.ncbi.nlm.nih.gov/pubmed/22724005
http://dx.doi.org/10.1371/journal.pone.0039365
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