The E89K Mutation in the Matrix Protein of the Measles Virus Affects In Vitro Cell Death and Virus Replication Efficiency in Human PBMC

Matrix protein is known to have an important role in the process of virus assembly and virion release during measles virus replication. In the present in vitro study, a single mutation of E89K in the matrix protein was shown to affect cell death and virus replication efficiency in human PBMC. One st...

Descripción completa

Detalles Bibliográficos
Autores principales: Dong, Jianbao, Zhu, Wei, Saito, Akatsuki, Goto, Yoshitaka, Iwata, Hiroyuki, Haga, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Open 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3377886/
https://www.ncbi.nlm.nih.gov/pubmed/22715352
http://dx.doi.org/10.2174/1874357901206010068
_version_ 1782235998406574080
author Dong, Jianbao
Zhu, Wei
Saito, Akatsuki
Goto, Yoshitaka
Iwata, Hiroyuki
Haga, Takeshi
author_facet Dong, Jianbao
Zhu, Wei
Saito, Akatsuki
Goto, Yoshitaka
Iwata, Hiroyuki
Haga, Takeshi
author_sort Dong, Jianbao
collection PubMed
description Matrix protein is known to have an important role in the process of virus assembly and virion release during measles virus replication. In the present in vitro study, a single mutation of E89K in the matrix protein was shown to affect cell death and virus replication efficiency in human PBMC. One strain with this mutation caused less cell death than the parental virus, and possessed high virus replication efficiency. Moreover, by Annexin V-FITC staining, polycaspase FLICA staining, and double labeling with poly-caspase FLICA and the Hoechst stain, the cell death seen was shown to be apoptosis.
format Online
Article
Text
id pubmed-3377886
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Bentham Open
record_format MEDLINE/PubMed
spelling pubmed-33778862012-06-19 The E89K Mutation in the Matrix Protein of the Measles Virus Affects In Vitro Cell Death and Virus Replication Efficiency in Human PBMC Dong, Jianbao Zhu, Wei Saito, Akatsuki Goto, Yoshitaka Iwata, Hiroyuki Haga, Takeshi Open Virol J Article Matrix protein is known to have an important role in the process of virus assembly and virion release during measles virus replication. In the present in vitro study, a single mutation of E89K in the matrix protein was shown to affect cell death and virus replication efficiency in human PBMC. One strain with this mutation caused less cell death than the parental virus, and possessed high virus replication efficiency. Moreover, by Annexin V-FITC staining, polycaspase FLICA staining, and double labeling with poly-caspase FLICA and the Hoechst stain, the cell death seen was shown to be apoptosis. Bentham Open 2012-06-08 /pmc/articles/PMC3377886/ /pubmed/22715352 http://dx.doi.org/10.2174/1874357901206010068 Text en © Dong et al.; Licensee Bentham Open. http://creativecommons.org/licenses/by-nc/3.0/ This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Article
Dong, Jianbao
Zhu, Wei
Saito, Akatsuki
Goto, Yoshitaka
Iwata, Hiroyuki
Haga, Takeshi
The E89K Mutation in the Matrix Protein of the Measles Virus Affects In Vitro Cell Death and Virus Replication Efficiency in Human PBMC
title The E89K Mutation in the Matrix Protein of the Measles Virus Affects In Vitro Cell Death and Virus Replication Efficiency in Human PBMC
title_full The E89K Mutation in the Matrix Protein of the Measles Virus Affects In Vitro Cell Death and Virus Replication Efficiency in Human PBMC
title_fullStr The E89K Mutation in the Matrix Protein of the Measles Virus Affects In Vitro Cell Death and Virus Replication Efficiency in Human PBMC
title_full_unstemmed The E89K Mutation in the Matrix Protein of the Measles Virus Affects In Vitro Cell Death and Virus Replication Efficiency in Human PBMC
title_short The E89K Mutation in the Matrix Protein of the Measles Virus Affects In Vitro Cell Death and Virus Replication Efficiency in Human PBMC
title_sort e89k mutation in the matrix protein of the measles virus affects in vitro cell death and virus replication efficiency in human pbmc
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3377886/
https://www.ncbi.nlm.nih.gov/pubmed/22715352
http://dx.doi.org/10.2174/1874357901206010068
work_keys_str_mv AT dongjianbao thee89kmutationinthematrixproteinofthemeaslesvirusaffectsinvitrocelldeathandvirusreplicationefficiencyinhumanpbmc
AT zhuwei thee89kmutationinthematrixproteinofthemeaslesvirusaffectsinvitrocelldeathandvirusreplicationefficiencyinhumanpbmc
AT saitoakatsuki thee89kmutationinthematrixproteinofthemeaslesvirusaffectsinvitrocelldeathandvirusreplicationefficiencyinhumanpbmc
AT gotoyoshitaka thee89kmutationinthematrixproteinofthemeaslesvirusaffectsinvitrocelldeathandvirusreplicationefficiencyinhumanpbmc
AT iwatahiroyuki thee89kmutationinthematrixproteinofthemeaslesvirusaffectsinvitrocelldeathandvirusreplicationefficiencyinhumanpbmc
AT hagatakeshi thee89kmutationinthematrixproteinofthemeaslesvirusaffectsinvitrocelldeathandvirusreplicationefficiencyinhumanpbmc
AT dongjianbao e89kmutationinthematrixproteinofthemeaslesvirusaffectsinvitrocelldeathandvirusreplicationefficiencyinhumanpbmc
AT zhuwei e89kmutationinthematrixproteinofthemeaslesvirusaffectsinvitrocelldeathandvirusreplicationefficiencyinhumanpbmc
AT saitoakatsuki e89kmutationinthematrixproteinofthemeaslesvirusaffectsinvitrocelldeathandvirusreplicationefficiencyinhumanpbmc
AT gotoyoshitaka e89kmutationinthematrixproteinofthemeaslesvirusaffectsinvitrocelldeathandvirusreplicationefficiencyinhumanpbmc
AT iwatahiroyuki e89kmutationinthematrixproteinofthemeaslesvirusaffectsinvitrocelldeathandvirusreplicationefficiencyinhumanpbmc
AT hagatakeshi e89kmutationinthematrixproteinofthemeaslesvirusaffectsinvitrocelldeathandvirusreplicationefficiencyinhumanpbmc

Ejemplares similares