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MEMS-based handheld confocal microscope for in-vivo skin imaging
This paper describes a handheld laser scanning confocal microscope for skin microscopy. Beam scanning is accomplished with an electromagnetic MEMS bi-axial micromirror developed for pico projector applications, providing 800x600 (SVGA) resolution at 56 frames per second. The design uses commercial o...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378354/ https://www.ncbi.nlm.nih.gov/pubmed/20389391 http://dx.doi.org/10.1364/OE.18.003805 |
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author | Arrasmith, Christopher L. Dickensheets, David L. Mahadevan-Jansen, Anita |
author_facet | Arrasmith, Christopher L. Dickensheets, David L. Mahadevan-Jansen, Anita |
author_sort | Arrasmith, Christopher L. |
collection | PubMed |
description | This paper describes a handheld laser scanning confocal microscope for skin microscopy. Beam scanning is accomplished with an electromagnetic MEMS bi-axial micromirror developed for pico projector applications, providing 800x600 (SVGA) resolution at 56 frames per second. The design uses commercial objective lenses with an optional hemisphere front lens, operating with a range of numerical aperture from NA=0.35 to NA=1.1 and corresponding diagonal field of view ranging from 653 μm to 216 μm. Using NA=1.1 and a laser wavelength of 830 nm we measured the axial response to be 1.14 μm full width at half maximum, with a corresponding 10%-90% lateral edge response of 0.39 μm. Image examples showing both epidermal and dermal features including capillary blood flow are provided. These images represent the highest resolution and frame rate yet achieved for tissue imaging with a MEMS bi-axial scan mirror. |
format | Online Article Text |
id | pubmed-3378354 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-33783542012-10-01 MEMS-based handheld confocal microscope for in-vivo skin imaging Arrasmith, Christopher L. Dickensheets, David L. Mahadevan-Jansen, Anita Opt Express Research-Article This paper describes a handheld laser scanning confocal microscope for skin microscopy. Beam scanning is accomplished with an electromagnetic MEMS bi-axial micromirror developed for pico projector applications, providing 800x600 (SVGA) resolution at 56 frames per second. The design uses commercial objective lenses with an optional hemisphere front lens, operating with a range of numerical aperture from NA=0.35 to NA=1.1 and corresponding diagonal field of view ranging from 653 μm to 216 μm. Using NA=1.1 and a laser wavelength of 830 nm we measured the axial response to be 1.14 μm full width at half maximum, with a corresponding 10%-90% lateral edge response of 0.39 μm. Image examples showing both epidermal and dermal features including capillary blood flow are provided. These images represent the highest resolution and frame rate yet achieved for tissue imaging with a MEMS bi-axial scan mirror. Optical Society of America 2010-02-11 /pmc/articles/PMC3378354/ /pubmed/20389391 http://dx.doi.org/10.1364/OE.18.003805 Text en ©2010 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Research-Article Arrasmith, Christopher L. Dickensheets, David L. Mahadevan-Jansen, Anita MEMS-based handheld confocal microscope for in-vivo skin imaging |
title | MEMS-based handheld confocal microscope for in-vivo skin imaging |
title_full | MEMS-based handheld confocal microscope for in-vivo skin imaging |
title_fullStr | MEMS-based handheld confocal microscope for in-vivo skin imaging |
title_full_unstemmed | MEMS-based handheld confocal microscope for in-vivo skin imaging |
title_short | MEMS-based handheld confocal microscope for in-vivo skin imaging |
title_sort | mems-based handheld confocal microscope for in-vivo skin imaging |
topic | Research-Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378354/ https://www.ncbi.nlm.nih.gov/pubmed/20389391 http://dx.doi.org/10.1364/OE.18.003805 |
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