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MEMS-based handheld confocal microscope for in-vivo skin imaging

This paper describes a handheld laser scanning confocal microscope for skin microscopy. Beam scanning is accomplished with an electromagnetic MEMS bi-axial micromirror developed for pico projector applications, providing 800x600 (SVGA) resolution at 56 frames per second. The design uses commercial o...

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Detalles Bibliográficos
Autores principales: Arrasmith, Christopher L., Dickensheets, David L., Mahadevan-Jansen, Anita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378354/
https://www.ncbi.nlm.nih.gov/pubmed/20389391
http://dx.doi.org/10.1364/OE.18.003805
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author Arrasmith, Christopher L.
Dickensheets, David L.
Mahadevan-Jansen, Anita
author_facet Arrasmith, Christopher L.
Dickensheets, David L.
Mahadevan-Jansen, Anita
author_sort Arrasmith, Christopher L.
collection PubMed
description This paper describes a handheld laser scanning confocal microscope for skin microscopy. Beam scanning is accomplished with an electromagnetic MEMS bi-axial micromirror developed for pico projector applications, providing 800x600 (SVGA) resolution at 56 frames per second. The design uses commercial objective lenses with an optional hemisphere front lens, operating with a range of numerical aperture from NA=0.35 to NA=1.1 and corresponding diagonal field of view ranging from 653 μm to 216 μm. Using NA=1.1 and a laser wavelength of 830 nm we measured the axial response to be 1.14 μm full width at half maximum, with a corresponding 10%-90% lateral edge response of 0.39 μm. Image examples showing both epidermal and dermal features including capillary blood flow are provided. These images represent the highest resolution and frame rate yet achieved for tissue imaging with a MEMS bi-axial scan mirror.
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spelling pubmed-33783542012-10-01 MEMS-based handheld confocal microscope for in-vivo skin imaging Arrasmith, Christopher L. Dickensheets, David L. Mahadevan-Jansen, Anita Opt Express Research-Article This paper describes a handheld laser scanning confocal microscope for skin microscopy. Beam scanning is accomplished with an electromagnetic MEMS bi-axial micromirror developed for pico projector applications, providing 800x600 (SVGA) resolution at 56 frames per second. The design uses commercial objective lenses with an optional hemisphere front lens, operating with a range of numerical aperture from NA=0.35 to NA=1.1 and corresponding diagonal field of view ranging from 653 μm to 216 μm. Using NA=1.1 and a laser wavelength of 830 nm we measured the axial response to be 1.14 μm full width at half maximum, with a corresponding 10%-90% lateral edge response of 0.39 μm. Image examples showing both epidermal and dermal features including capillary blood flow are provided. These images represent the highest resolution and frame rate yet achieved for tissue imaging with a MEMS bi-axial scan mirror. Optical Society of America 2010-02-11 /pmc/articles/PMC3378354/ /pubmed/20389391 http://dx.doi.org/10.1364/OE.18.003805 Text en ©2010 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Research-Article
Arrasmith, Christopher L.
Dickensheets, David L.
Mahadevan-Jansen, Anita
MEMS-based handheld confocal microscope for in-vivo skin imaging
title MEMS-based handheld confocal microscope for in-vivo skin imaging
title_full MEMS-based handheld confocal microscope for in-vivo skin imaging
title_fullStr MEMS-based handheld confocal microscope for in-vivo skin imaging
title_full_unstemmed MEMS-based handheld confocal microscope for in-vivo skin imaging
title_short MEMS-based handheld confocal microscope for in-vivo skin imaging
title_sort mems-based handheld confocal microscope for in-vivo skin imaging
topic Research-Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378354/
https://www.ncbi.nlm.nih.gov/pubmed/20389391
http://dx.doi.org/10.1364/OE.18.003805
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