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The immune evasion protein Sbi of Staphylococcus aureus occurs both extracellularly and anchored to the cell envelope by binding lipoteichoic acid

The Sbi protein of Staphylococcus aureus comprises two IgG-binding domains similar to those of protein A and a region that triggers the activation of complement C3. Sbi is expressed on the cell surface but its C-terminal domain lacks motifs associated with wall or membrane anchoring of proteins in G...

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Autores principales: Smith, Emma Jane, Corrigan, Rebecca M, van der Sluis, Tetje, Gründling, Angelika, Speziale, Pietro, Geoghegan, Joan A, Foster, Timothy J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378709/
https://www.ncbi.nlm.nih.gov/pubmed/22256861
http://dx.doi.org/10.1111/j.1365-2958.2011.07966.x
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author Smith, Emma Jane
Corrigan, Rebecca M
van der Sluis, Tetje
Gründling, Angelika
Speziale, Pietro
Geoghegan, Joan A
Foster, Timothy J
author_facet Smith, Emma Jane
Corrigan, Rebecca M
van der Sluis, Tetje
Gründling, Angelika
Speziale, Pietro
Geoghegan, Joan A
Foster, Timothy J
author_sort Smith, Emma Jane
collection PubMed
description The Sbi protein of Staphylococcus aureus comprises two IgG-binding domains similar to those of protein A and a region that triggers the activation of complement C3. Sbi is expressed on the cell surface but its C-terminal domain lacks motifs associated with wall or membrane anchoring of proteins in Gram-positive bacteria. Cell-associated Sbi fractionates with the cytoplasmic membrane and is not solubilized during protoplast formation. S. aureus expressing Sbi truncates of the C-terminal Y domain allowed identification of residues that are required for association of Sbi with the membrane. Recombinant Sbi bound to purified cytoplasmic membrane material in vitro and to purified lipoteichoic acid. This explains how Sbi partitions with the membrane in fractionation experiments yet is partially exposed on the cell surface. An LTA-defective mutant of S. aureus had reduced levels of Sbi in the cytoplasmic membrane.
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spelling pubmed-33787092012-06-20 The immune evasion protein Sbi of Staphylococcus aureus occurs both extracellularly and anchored to the cell envelope by binding lipoteichoic acid Smith, Emma Jane Corrigan, Rebecca M van der Sluis, Tetje Gründling, Angelika Speziale, Pietro Geoghegan, Joan A Foster, Timothy J Mol Microbiol Research Articles The Sbi protein of Staphylococcus aureus comprises two IgG-binding domains similar to those of protein A and a region that triggers the activation of complement C3. Sbi is expressed on the cell surface but its C-terminal domain lacks motifs associated with wall or membrane anchoring of proteins in Gram-positive bacteria. Cell-associated Sbi fractionates with the cytoplasmic membrane and is not solubilized during protoplast formation. S. aureus expressing Sbi truncates of the C-terminal Y domain allowed identification of residues that are required for association of Sbi with the membrane. Recombinant Sbi bound to purified cytoplasmic membrane material in vitro and to purified lipoteichoic acid. This explains how Sbi partitions with the membrane in fractionation experiments yet is partially exposed on the cell surface. An LTA-defective mutant of S. aureus had reduced levels of Sbi in the cytoplasmic membrane. Blackwell Publishing Ltd 2012-02 2012-01-18 /pmc/articles/PMC3378709/ /pubmed/22256861 http://dx.doi.org/10.1111/j.1365-2958.2011.07966.x Text en © 2012 Blackwell Publishing Ltd http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Research Articles
Smith, Emma Jane
Corrigan, Rebecca M
van der Sluis, Tetje
Gründling, Angelika
Speziale, Pietro
Geoghegan, Joan A
Foster, Timothy J
The immune evasion protein Sbi of Staphylococcus aureus occurs both extracellularly and anchored to the cell envelope by binding lipoteichoic acid
title The immune evasion protein Sbi of Staphylococcus aureus occurs both extracellularly and anchored to the cell envelope by binding lipoteichoic acid
title_full The immune evasion protein Sbi of Staphylococcus aureus occurs both extracellularly and anchored to the cell envelope by binding lipoteichoic acid
title_fullStr The immune evasion protein Sbi of Staphylococcus aureus occurs both extracellularly and anchored to the cell envelope by binding lipoteichoic acid
title_full_unstemmed The immune evasion protein Sbi of Staphylococcus aureus occurs both extracellularly and anchored to the cell envelope by binding lipoteichoic acid
title_short The immune evasion protein Sbi of Staphylococcus aureus occurs both extracellularly and anchored to the cell envelope by binding lipoteichoic acid
title_sort immune evasion protein sbi of staphylococcus aureus occurs both extracellularly and anchored to the cell envelope by binding lipoteichoic acid
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378709/
https://www.ncbi.nlm.nih.gov/pubmed/22256861
http://dx.doi.org/10.1111/j.1365-2958.2011.07966.x
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