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DNA helicase and helicase–nuclease enzymes with a conserved iron–sulfur cluster
Conserved Iron–Sulfur (Fe–S) clusters are found in a growing family of metalloproteins that are implicated in prokaryotic and eukaryotic DNA replication and repair. Among these are DNA helicase and helicase–nuclease enzymes that preserve chromosomal stability and are genetically linked to diseases c...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378879/ https://www.ncbi.nlm.nih.gov/pubmed/22287629 http://dx.doi.org/10.1093/nar/gks039 |
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author | Wu, Yuliang Brosh, Robert M. |
author_facet | Wu, Yuliang Brosh, Robert M. |
author_sort | Wu, Yuliang |
collection | PubMed |
description | Conserved Iron–Sulfur (Fe–S) clusters are found in a growing family of metalloproteins that are implicated in prokaryotic and eukaryotic DNA replication and repair. Among these are DNA helicase and helicase–nuclease enzymes that preserve chromosomal stability and are genetically linked to diseases characterized by DNA repair defects and/or a poor response to replication stress. Insight to the structural and functional importance of the conserved Fe–S domain in DNA helicases has been gleaned from structural studies of the purified proteins and characterization of Fe–S cluster site-directed mutants. In this review, we will provide a current perspective of what is known about the Fe–S cluster helicases, with an emphasis on how the conserved redox active domain may facilitate mechanistic aspects of helicase function. We will discuss testable models for how the conserved Fe–S cluster might operate in helicase and helicase–nuclease enzymes to conduct their specialized functions that help to preserve the integrity of the genome. |
format | Online Article Text |
id | pubmed-3378879 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-33788792012-06-20 DNA helicase and helicase–nuclease enzymes with a conserved iron–sulfur cluster Wu, Yuliang Brosh, Robert M. Nucleic Acids Res Survey and Summary Conserved Iron–Sulfur (Fe–S) clusters are found in a growing family of metalloproteins that are implicated in prokaryotic and eukaryotic DNA replication and repair. Among these are DNA helicase and helicase–nuclease enzymes that preserve chromosomal stability and are genetically linked to diseases characterized by DNA repair defects and/or a poor response to replication stress. Insight to the structural and functional importance of the conserved Fe–S domain in DNA helicases has been gleaned from structural studies of the purified proteins and characterization of Fe–S cluster site-directed mutants. In this review, we will provide a current perspective of what is known about the Fe–S cluster helicases, with an emphasis on how the conserved redox active domain may facilitate mechanistic aspects of helicase function. We will discuss testable models for how the conserved Fe–S cluster might operate in helicase and helicase–nuclease enzymes to conduct their specialized functions that help to preserve the integrity of the genome. Oxford University Press 2012-05 2012-01-28 /pmc/articles/PMC3378879/ /pubmed/22287629 http://dx.doi.org/10.1093/nar/gks039 Text en Published by Oxford University Press 2012. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Survey and Summary Wu, Yuliang Brosh, Robert M. DNA helicase and helicase–nuclease enzymes with a conserved iron–sulfur cluster |
title | DNA helicase and helicase–nuclease enzymes with a conserved iron–sulfur cluster |
title_full | DNA helicase and helicase–nuclease enzymes with a conserved iron–sulfur cluster |
title_fullStr | DNA helicase and helicase–nuclease enzymes with a conserved iron–sulfur cluster |
title_full_unstemmed | DNA helicase and helicase–nuclease enzymes with a conserved iron–sulfur cluster |
title_short | DNA helicase and helicase–nuclease enzymes with a conserved iron–sulfur cluster |
title_sort | dna helicase and helicase–nuclease enzymes with a conserved iron–sulfur cluster |
topic | Survey and Summary |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378879/ https://www.ncbi.nlm.nih.gov/pubmed/22287629 http://dx.doi.org/10.1093/nar/gks039 |
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