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A novel non-homologous recombination-mediated mechanism for Escherichia coli unilateral flagellar phase variation

Flagella contribute to the virulence of bacteria through chemotaxis, adhesion to and invasion of host surfaces. Flagellar phase variation is believed to facilitate bacterial evasion of the host immune response. In this study, the flnA gene that encodes Escherichia coli H17 flagellin was examined by...

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Autores principales: Liu, Bin, Hu, Bo, Zhou, Zhemin, Guo, Dan, Guo, Xi, Ding, Peng, Feng, Lu, Wang, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378880/
https://www.ncbi.nlm.nih.gov/pubmed/22287625
http://dx.doi.org/10.1093/nar/gks040
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author Liu, Bin
Hu, Bo
Zhou, Zhemin
Guo, Dan
Guo, Xi
Ding, Peng
Feng, Lu
Wang, Lei
author_facet Liu, Bin
Hu, Bo
Zhou, Zhemin
Guo, Dan
Guo, Xi
Ding, Peng
Feng, Lu
Wang, Lei
author_sort Liu, Bin
collection PubMed
description Flagella contribute to the virulence of bacteria through chemotaxis, adhesion to and invasion of host surfaces. Flagellar phase variation is believed to facilitate bacterial evasion of the host immune response. In this study, the flnA gene that encodes Escherichia coli H17 flagellin was examined by whole genome sequencing and genetic deletion analysis. Unilateral flagellar phase variation has been reported in E. coli H3, H47 and H17 strains, although the mechanism for phase variation in the H17 strain has not been previously understood. Analysis of phase variants indicated that the flagellar phase variation in the H17 strain was caused by the deletion of an ∼35 kb DNA region containing the flnA gene from diverse excision sites. The presence of covalently closed extrachromosomal circular forms of this excised 35 kb region was confirmed by the two-step polymerase chain reaction. The deletion and complementation test revealed that the Int1157 integrase, a tyrosine recombinase, mediates the excision of this region. Unlike most tyrosine recombinases, Int1157 is suggested to recognize diverse sites and mediate recombination between non-homologous DNA sequences. This is the first report of non-homologous recombination mediating flagellar phase variation.
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spelling pubmed-33788802012-06-20 A novel non-homologous recombination-mediated mechanism for Escherichia coli unilateral flagellar phase variation Liu, Bin Hu, Bo Zhou, Zhemin Guo, Dan Guo, Xi Ding, Peng Feng, Lu Wang, Lei Nucleic Acids Res Molecular Biology Flagella contribute to the virulence of bacteria through chemotaxis, adhesion to and invasion of host surfaces. Flagellar phase variation is believed to facilitate bacterial evasion of the host immune response. In this study, the flnA gene that encodes Escherichia coli H17 flagellin was examined by whole genome sequencing and genetic deletion analysis. Unilateral flagellar phase variation has been reported in E. coli H3, H47 and H17 strains, although the mechanism for phase variation in the H17 strain has not been previously understood. Analysis of phase variants indicated that the flagellar phase variation in the H17 strain was caused by the deletion of an ∼35 kb DNA region containing the flnA gene from diverse excision sites. The presence of covalently closed extrachromosomal circular forms of this excised 35 kb region was confirmed by the two-step polymerase chain reaction. The deletion and complementation test revealed that the Int1157 integrase, a tyrosine recombinase, mediates the excision of this region. Unlike most tyrosine recombinases, Int1157 is suggested to recognize diverse sites and mediate recombination between non-homologous DNA sequences. This is the first report of non-homologous recombination mediating flagellar phase variation. Oxford University Press 2012-05 2012-01-28 /pmc/articles/PMC3378880/ /pubmed/22287625 http://dx.doi.org/10.1093/nar/gks040 Text en © The Author(s) 2012. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Molecular Biology
Liu, Bin
Hu, Bo
Zhou, Zhemin
Guo, Dan
Guo, Xi
Ding, Peng
Feng, Lu
Wang, Lei
A novel non-homologous recombination-mediated mechanism for Escherichia coli unilateral flagellar phase variation
title A novel non-homologous recombination-mediated mechanism for Escherichia coli unilateral flagellar phase variation
title_full A novel non-homologous recombination-mediated mechanism for Escherichia coli unilateral flagellar phase variation
title_fullStr A novel non-homologous recombination-mediated mechanism for Escherichia coli unilateral flagellar phase variation
title_full_unstemmed A novel non-homologous recombination-mediated mechanism for Escherichia coli unilateral flagellar phase variation
title_short A novel non-homologous recombination-mediated mechanism for Escherichia coli unilateral flagellar phase variation
title_sort novel non-homologous recombination-mediated mechanism for escherichia coli unilateral flagellar phase variation
topic Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378880/
https://www.ncbi.nlm.nih.gov/pubmed/22287625
http://dx.doi.org/10.1093/nar/gks040
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