Bromopyridazinedione-mediated protein and peptide bioconjugation

Bromopyridazinedione-mediated bioconjugation to a cysteine containing protein and a disulfide containing peptide is described. The conjugates are cleavable in an excess of thiol, including cytoplasmically-relevant concentrations of glutathione, and show a high level of hydrolytic stability. The cons...

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Autores principales: Chudasama, Vijay, Smith, Mark E. B., Schumacher, Felix F., Papaioannou, Danai, Waksman, Gabriel, Baker, James R., Caddick, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2011
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3379626/
https://www.ncbi.nlm.nih.gov/pubmed/21738916
http://dx.doi.org/10.1039/c1cc12807h
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author Chudasama, Vijay
Smith, Mark E. B.
Schumacher, Felix F.
Papaioannou, Danai
Waksman, Gabriel
Baker, James R.
Caddick, Stephen
author_facet Chudasama, Vijay
Smith, Mark E. B.
Schumacher, Felix F.
Papaioannou, Danai
Waksman, Gabriel
Baker, James R.
Caddick, Stephen
author_sort Chudasama, Vijay
collection PubMed
description Bromopyridazinedione-mediated bioconjugation to a cysteine containing protein and a disulfide containing peptide is described. The conjugates are cleavable in an excess of thiol, including cytoplasmically-relevant concentrations of glutathione, and show a high level of hydrolytic stability. The constructs have the potential for four points of chemical attachment.
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spelling pubmed-33796262012-06-20 Bromopyridazinedione-mediated protein and peptide bioconjugation Chudasama, Vijay Smith, Mark E. B. Schumacher, Felix F. Papaioannou, Danai Waksman, Gabriel Baker, James R. Caddick, Stephen Chem Commun (Camb) Chemistry Bromopyridazinedione-mediated bioconjugation to a cysteine containing protein and a disulfide containing peptide is described. The conjugates are cleavable in an excess of thiol, including cytoplasmically-relevant concentrations of glutathione, and show a high level of hydrolytic stability. The constructs have the potential for four points of chemical attachment. Royal Society of Chemistry 2011-08-21 2011-07-07 /pmc/articles/PMC3379626/ /pubmed/21738916 http://dx.doi.org/10.1039/c1cc12807h Text en This journal is © The Royal Society of Chemistry 2011 http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Chudasama, Vijay
Smith, Mark E. B.
Schumacher, Felix F.
Papaioannou, Danai
Waksman, Gabriel
Baker, James R.
Caddick, Stephen
Bromopyridazinedione-mediated protein and peptide bioconjugation
title Bromopyridazinedione-mediated protein and peptide bioconjugation
title_full Bromopyridazinedione-mediated protein and peptide bioconjugation
title_fullStr Bromopyridazinedione-mediated protein and peptide bioconjugation
title_full_unstemmed Bromopyridazinedione-mediated protein and peptide bioconjugation
title_short Bromopyridazinedione-mediated protein and peptide bioconjugation
title_sort bromopyridazinedione-mediated protein and peptide bioconjugation
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3379626/
https://www.ncbi.nlm.nih.gov/pubmed/21738916
http://dx.doi.org/10.1039/c1cc12807h
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