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CC8 MRSA Strains Harboring SCCmec Type IVc are Predominant in Colombian Hospitals

BACKGROUND: Recent reports highlight the incursion of community-associated MRSA within healthcare settings. However, knowledge of this phenomenon remains limited in Latin America. The aim of this study was to evaluate the molecular epidemiology of MRSA in three tertiary-care hospitals in Medellín, C...

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Autores principales: Jiménez, J. Natalia, Ocampo, Ana M., Vanegas, Johanna M., Rodriguez, Erika A., Mediavilla, José R., Chen, Liang, Muskus, Carlos E., A. Vélez, Lázaro, Rojas, Carlos, Restrepo, Andrea V., Ospina, Sigifredo, Garcés, Carlos, Franco, Liliana, Bifani, Pablo, Kreiswirth, Barry N., Correa, Margarita M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3380008/
https://www.ncbi.nlm.nih.gov/pubmed/22745670
http://dx.doi.org/10.1371/journal.pone.0038576
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author Jiménez, J. Natalia
Ocampo, Ana M.
Vanegas, Johanna M.
Rodriguez, Erika A.
Mediavilla, José R.
Chen, Liang
Muskus, Carlos E.
A. Vélez, Lázaro
Rojas, Carlos
Restrepo, Andrea V.
Ospina, Sigifredo
Garcés, Carlos
Franco, Liliana
Bifani, Pablo
Kreiswirth, Barry N.
Correa, Margarita M.
author_facet Jiménez, J. Natalia
Ocampo, Ana M.
Vanegas, Johanna M.
Rodriguez, Erika A.
Mediavilla, José R.
Chen, Liang
Muskus, Carlos E.
A. Vélez, Lázaro
Rojas, Carlos
Restrepo, Andrea V.
Ospina, Sigifredo
Garcés, Carlos
Franco, Liliana
Bifani, Pablo
Kreiswirth, Barry N.
Correa, Margarita M.
author_sort Jiménez, J. Natalia
collection PubMed
description BACKGROUND: Recent reports highlight the incursion of community-associated MRSA within healthcare settings. However, knowledge of this phenomenon remains limited in Latin America. The aim of this study was to evaluate the molecular epidemiology of MRSA in three tertiary-care hospitals in Medellín, Colombia. METHODS: An observational cross-sectional study was conducted from 2008–2010. MRSA infections were classified as either community-associated (CA-MRSA) or healthcare-associated (HA-MRSA), with HA-MRSA further classified as hospital-onset (HAHO-MRSA) or community-onset (HACO-MRSA) according to standard epidemiological definitions established by the U.S. Centers for Disease Control and Prevention (CDC). Genotypic analysis included SCCmec typing, spa typing, PFGE and MLST. RESULTS: Out of 538 total MRSA isolates, 68 (12.6%) were defined as CA-MRSA, 243 (45.2%) as HACO-MRSA and 227 (42.2%) as HAHO-MRSA. The majority harbored SCCmec type IVc (306, 58.7%), followed by SCCmec type I (174, 33.4%). The prevalence of type IVc among CA-, HACO- and HAHO-MRSA isolates was 92.4%, 65.1% and 43.6%, respectively. From 2008 to 2010, the prevalence of type IVc-bearing strains increased significantly, from 50.0% to 68.2% (p = 0.004). Strains harboring SCCmec IVc were mainly associated with spa types t1610, t008 and t024 (MLST clonal complex 8), while PFGE confirmed that the t008 and t1610 strains were closely related to the USA300-0114 CA-MRSA clone. Notably, strains belonging to these three spa types exhibited high levels of tetracycline resistance (45.9%). CONCLUSION: CC8 MRSA strains harboring SCCmec type IVc are becoming predominant in Medellín hospitals, displacing previously reported CC5 HA-MRSA clones. Based on shared characteristics including SCCmec IVc, absence of the ACME element and tetracycline resistance, the USA300-related isolates in this study are most likely related to USA300-LV, the recently-described ‘Latin American variant’ of USA300.
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spelling pubmed-33800082012-06-28 CC8 MRSA Strains Harboring SCCmec Type IVc are Predominant in Colombian Hospitals Jiménez, J. Natalia Ocampo, Ana M. Vanegas, Johanna M. Rodriguez, Erika A. Mediavilla, José R. Chen, Liang Muskus, Carlos E. A. Vélez, Lázaro Rojas, Carlos Restrepo, Andrea V. Ospina, Sigifredo Garcés, Carlos Franco, Liliana Bifani, Pablo Kreiswirth, Barry N. Correa, Margarita M. PLoS One Research Article BACKGROUND: Recent reports highlight the incursion of community-associated MRSA within healthcare settings. However, knowledge of this phenomenon remains limited in Latin America. The aim of this study was to evaluate the molecular epidemiology of MRSA in three tertiary-care hospitals in Medellín, Colombia. METHODS: An observational cross-sectional study was conducted from 2008–2010. MRSA infections were classified as either community-associated (CA-MRSA) or healthcare-associated (HA-MRSA), with HA-MRSA further classified as hospital-onset (HAHO-MRSA) or community-onset (HACO-MRSA) according to standard epidemiological definitions established by the U.S. Centers for Disease Control and Prevention (CDC). Genotypic analysis included SCCmec typing, spa typing, PFGE and MLST. RESULTS: Out of 538 total MRSA isolates, 68 (12.6%) were defined as CA-MRSA, 243 (45.2%) as HACO-MRSA and 227 (42.2%) as HAHO-MRSA. The majority harbored SCCmec type IVc (306, 58.7%), followed by SCCmec type I (174, 33.4%). The prevalence of type IVc among CA-, HACO- and HAHO-MRSA isolates was 92.4%, 65.1% and 43.6%, respectively. From 2008 to 2010, the prevalence of type IVc-bearing strains increased significantly, from 50.0% to 68.2% (p = 0.004). Strains harboring SCCmec IVc were mainly associated with spa types t1610, t008 and t024 (MLST clonal complex 8), while PFGE confirmed that the t008 and t1610 strains were closely related to the USA300-0114 CA-MRSA clone. Notably, strains belonging to these three spa types exhibited high levels of tetracycline resistance (45.9%). CONCLUSION: CC8 MRSA strains harboring SCCmec type IVc are becoming predominant in Medellín hospitals, displacing previously reported CC5 HA-MRSA clones. Based on shared characteristics including SCCmec IVc, absence of the ACME element and tetracycline resistance, the USA300-related isolates in this study are most likely related to USA300-LV, the recently-described ‘Latin American variant’ of USA300. Public Library of Science 2012-06-20 /pmc/articles/PMC3380008/ /pubmed/22745670 http://dx.doi.org/10.1371/journal.pone.0038576 Text en Jiménez et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Jiménez, J. Natalia
Ocampo, Ana M.
Vanegas, Johanna M.
Rodriguez, Erika A.
Mediavilla, José R.
Chen, Liang
Muskus, Carlos E.
A. Vélez, Lázaro
Rojas, Carlos
Restrepo, Andrea V.
Ospina, Sigifredo
Garcés, Carlos
Franco, Liliana
Bifani, Pablo
Kreiswirth, Barry N.
Correa, Margarita M.
CC8 MRSA Strains Harboring SCCmec Type IVc are Predominant in Colombian Hospitals
title CC8 MRSA Strains Harboring SCCmec Type IVc are Predominant in Colombian Hospitals
title_full CC8 MRSA Strains Harboring SCCmec Type IVc are Predominant in Colombian Hospitals
title_fullStr CC8 MRSA Strains Harboring SCCmec Type IVc are Predominant in Colombian Hospitals
title_full_unstemmed CC8 MRSA Strains Harboring SCCmec Type IVc are Predominant in Colombian Hospitals
title_short CC8 MRSA Strains Harboring SCCmec Type IVc are Predominant in Colombian Hospitals
title_sort cc8 mrsa strains harboring sccmec type ivc are predominant in colombian hospitals
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3380008/
https://www.ncbi.nlm.nih.gov/pubmed/22745670
http://dx.doi.org/10.1371/journal.pone.0038576
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