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Impact of Intestinal Microbiota on Intestinal Luminal Metabolome

Low–molecular-weight metabolites produced by intestinal microbiota play a direct role in health and disease. In this study, we analyzed the colonic luminal metabolome using capillary electrophoresis mass spectrometry with time-of-flight (CE-TOFMS) —a novel technique for analyzing and differentially...

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Autores principales: Matsumoto, Mitsuharu, Kibe, Ryoko, Ooga, Takushi, Aiba, Yuji, Kurihara, Shin, Sawaki, Emiko, Koga, Yasuhiro, Benno, Yoshimi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3380406/
https://www.ncbi.nlm.nih.gov/pubmed/22724057
http://dx.doi.org/10.1038/srep00233
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author Matsumoto, Mitsuharu
Kibe, Ryoko
Ooga, Takushi
Aiba, Yuji
Kurihara, Shin
Sawaki, Emiko
Koga, Yasuhiro
Benno, Yoshimi
author_facet Matsumoto, Mitsuharu
Kibe, Ryoko
Ooga, Takushi
Aiba, Yuji
Kurihara, Shin
Sawaki, Emiko
Koga, Yasuhiro
Benno, Yoshimi
author_sort Matsumoto, Mitsuharu
collection PubMed
description Low–molecular-weight metabolites produced by intestinal microbiota play a direct role in health and disease. In this study, we analyzed the colonic luminal metabolome using capillary electrophoresis mass spectrometry with time-of-flight (CE-TOFMS) —a novel technique for analyzing and differentially displaying metabolic profiles— in order to clarify the metabolite profiles in the intestinal lumen. CE-TOFMS identified 179 metabolites from the colonic luminal metabolome and 48 metabolites were present in significantly higher concentrations and/or incidence in the germ-free (GF) mice than in the Ex-GF mice (p < 0.05), 77 metabolites were present in significantly lower concentrations and/or incidence in the GF mice than in the Ex-GF mice (p < 0.05), and 56 metabolites showed no differences in the concentration or incidence between GF and Ex-GF mice. These indicate that intestinal microbiota highly influenced the colonic luminal metabolome and a comprehensive understanding of intestinal luminal metabolome is critical for clarifying host-intestinal bacterial interactions.
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spelling pubmed-33804062012-06-21 Impact of Intestinal Microbiota on Intestinal Luminal Metabolome Matsumoto, Mitsuharu Kibe, Ryoko Ooga, Takushi Aiba, Yuji Kurihara, Shin Sawaki, Emiko Koga, Yasuhiro Benno, Yoshimi Sci Rep Article Low–molecular-weight metabolites produced by intestinal microbiota play a direct role in health and disease. In this study, we analyzed the colonic luminal metabolome using capillary electrophoresis mass spectrometry with time-of-flight (CE-TOFMS) —a novel technique for analyzing and differentially displaying metabolic profiles— in order to clarify the metabolite profiles in the intestinal lumen. CE-TOFMS identified 179 metabolites from the colonic luminal metabolome and 48 metabolites were present in significantly higher concentrations and/or incidence in the germ-free (GF) mice than in the Ex-GF mice (p < 0.05), 77 metabolites were present in significantly lower concentrations and/or incidence in the GF mice than in the Ex-GF mice (p < 0.05), and 56 metabolites showed no differences in the concentration or incidence between GF and Ex-GF mice. These indicate that intestinal microbiota highly influenced the colonic luminal metabolome and a comprehensive understanding of intestinal luminal metabolome is critical for clarifying host-intestinal bacterial interactions. Nature Publishing Group 2012-01-25 /pmc/articles/PMC3380406/ /pubmed/22724057 http://dx.doi.org/10.1038/srep00233 Text en Copyright © 2012, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Article
Matsumoto, Mitsuharu
Kibe, Ryoko
Ooga, Takushi
Aiba, Yuji
Kurihara, Shin
Sawaki, Emiko
Koga, Yasuhiro
Benno, Yoshimi
Impact of Intestinal Microbiota on Intestinal Luminal Metabolome
title Impact of Intestinal Microbiota on Intestinal Luminal Metabolome
title_full Impact of Intestinal Microbiota on Intestinal Luminal Metabolome
title_fullStr Impact of Intestinal Microbiota on Intestinal Luminal Metabolome
title_full_unstemmed Impact of Intestinal Microbiota on Intestinal Luminal Metabolome
title_short Impact of Intestinal Microbiota on Intestinal Luminal Metabolome
title_sort impact of intestinal microbiota on intestinal luminal metabolome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3380406/
https://www.ncbi.nlm.nih.gov/pubmed/22724057
http://dx.doi.org/10.1038/srep00233
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