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Neurochemical Properties of Dental Primary Afferent Neurons

The long belief that dental primary afferent (DPA) neurons are entirely composed of nociceptive neurons has been challenged by several anatomical and functional investigations. In order to characterize non-nociceptivepopulation among DPA neurons, retrograde transport fluorescent dye was placed in up...

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Autores principales: Vang, Hue, Chung, Gehoon, Kim, Hyun Yeong, Park, Seok-Beom, Jung, Sung Jun, Kim, Joong-Soo, Oh, Seog Bae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Brain and Neural Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3381214/
https://www.ncbi.nlm.nih.gov/pubmed/22792027
http://dx.doi.org/10.5607/en.2012.21.2.68
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author Vang, Hue
Chung, Gehoon
Kim, Hyun Yeong
Park, Seok-Beom
Jung, Sung Jun
Kim, Joong-Soo
Oh, Seog Bae
author_facet Vang, Hue
Chung, Gehoon
Kim, Hyun Yeong
Park, Seok-Beom
Jung, Sung Jun
Kim, Joong-Soo
Oh, Seog Bae
author_sort Vang, Hue
collection PubMed
description The long belief that dental primary afferent (DPA) neurons are entirely composed of nociceptive neurons has been challenged by several anatomical and functional investigations. In order to characterize non-nociceptivepopulation among DPA neurons, retrograde transport fluorescent dye was placed in upper molars of rats and immunohistochemical detection of peripherin and neurofilament 200 in the labeled trigeminal ganglia was performed. As the results, majority ofDPA neurons were peripherin-expressing small-sized neurons, showing characteristic ofnociceptive C-fibers. However, 25.7% of DPA were stained with antibody against neurofilament 200, indicating significant portion of DPA neurons are related to large myelinated Aβ fibers. There were a small number of neurons thatexpressed both peripherin and neurofilament 200, suggestive of Aδ fibers. The possible transition of neurochemical properties by neuronal injury induced by retrograde labeling technique was ruled out by detection of minimal expression of neuronal injury marker, ATF-3. These results suggest that in addition to the large population of C-fiber-related nociceptive neurons, a subset of DPA neurons is myelinated large neurons, which is related to low-threshold mechanosensitive Aβ fibers. We suggest that these Aβ fiber-related neurons might play a role as mechanotransducers of fluid movement within dentinal tubules.
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spelling pubmed-33812142012-07-12 Neurochemical Properties of Dental Primary Afferent Neurons Vang, Hue Chung, Gehoon Kim, Hyun Yeong Park, Seok-Beom Jung, Sung Jun Kim, Joong-Soo Oh, Seog Bae Exp Neurobiol Original Article The long belief that dental primary afferent (DPA) neurons are entirely composed of nociceptive neurons has been challenged by several anatomical and functional investigations. In order to characterize non-nociceptivepopulation among DPA neurons, retrograde transport fluorescent dye was placed in upper molars of rats and immunohistochemical detection of peripherin and neurofilament 200 in the labeled trigeminal ganglia was performed. As the results, majority ofDPA neurons were peripherin-expressing small-sized neurons, showing characteristic ofnociceptive C-fibers. However, 25.7% of DPA were stained with antibody against neurofilament 200, indicating significant portion of DPA neurons are related to large myelinated Aβ fibers. There were a small number of neurons thatexpressed both peripherin and neurofilament 200, suggestive of Aδ fibers. The possible transition of neurochemical properties by neuronal injury induced by retrograde labeling technique was ruled out by detection of minimal expression of neuronal injury marker, ATF-3. These results suggest that in addition to the large population of C-fiber-related nociceptive neurons, a subset of DPA neurons is myelinated large neurons, which is related to low-threshold mechanosensitive Aβ fibers. We suggest that these Aβ fiber-related neurons might play a role as mechanotransducers of fluid movement within dentinal tubules. The Korean Society for Brain and Neural Science 2012-06 2012-04-23 /pmc/articles/PMC3381214/ /pubmed/22792027 http://dx.doi.org/10.5607/en.2012.21.2.68 Text en Copyright © Experimental Neurobiology 2012. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Vang, Hue
Chung, Gehoon
Kim, Hyun Yeong
Park, Seok-Beom
Jung, Sung Jun
Kim, Joong-Soo
Oh, Seog Bae
Neurochemical Properties of Dental Primary Afferent Neurons
title Neurochemical Properties of Dental Primary Afferent Neurons
title_full Neurochemical Properties of Dental Primary Afferent Neurons
title_fullStr Neurochemical Properties of Dental Primary Afferent Neurons
title_full_unstemmed Neurochemical Properties of Dental Primary Afferent Neurons
title_short Neurochemical Properties of Dental Primary Afferent Neurons
title_sort neurochemical properties of dental primary afferent neurons
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3381214/
https://www.ncbi.nlm.nih.gov/pubmed/22792027
http://dx.doi.org/10.5607/en.2012.21.2.68
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